Gene knockout Escherichia coli beneficial to recombinant protein exocytosis and application thereof

A technology of Escherichia coli and gene knockout, applied in the fields of application, genetic engineering, plant gene improvement, etc., can solve the problems of increasing downstream costs and low extracellular expression, and achieve the effects of low cost, enhanced extracellular secretion, and high efficiency
CN104845926AInactive Publication Date: 2015-08-19NANJING UNIV OF TECH
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
NANJING UNIV OF TECH
Publication Date
2015-08-19
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a gene knockout Escherichia coli beneficial to recombinant protein exocytosis and application thereof, relating to the field of modification of Escherichia coli genetic group genes. The gene knockout Escherichia coli beneficial to recombinant protein exocytosis is prepared by knocking out lipopolysaccharide synthesis related genes in Escherichia coli. The invention also provides a construction method of the gene knockout Escherichia coli and application of the gene knockout Escherichia coli in preparing extracellular soluble proteins. The preparation method is simple, and has the advantages of high efficiency and low cost. After the lipopolysaccharide-synthesized related genes are knocked out, the permeability of the pericellular membrane is enhanced, thereby obviously enhancing the recombinant protein exocytosis.
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Description

technical field

[0001] The invention relates to the field of genetically modified Escherichia coli, in particular to a gene-knockout Escherichia coli beneficial to the extracellular secretion of recombinant proteins and its application. Background technique

[0002] The E. coli system is suitable for the production of proteins that do not require post-translational modification. Since Escherichia coli lacks the cofactors required in the protein folding process, the intermediates tend to accumulate in large quantities to form inclusion body precipitates. In order to obtain active proteins, the inclusion bodies must undergo tedious processes such as renaturation. The fructosidase is expressed in the Escherichia coli system. The intracellular expression is large and inclusion bodies are easy to form, and the extracellular expression is very small, which significantly increases the downstream cost.

[0003] Lipopolysaccharide (LPS) is a unique structural and functional molecule...

Claims

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