Vaccine composition, and preparation method and applications thereof

A technology of vaccine composition and antigen, which is applied in the field of vaccine composition, can solve problems such as increased stress response of pig herds, unsatisfactory immune effect of single vaccine, cumbersome operation procedures, etc., and achieves less animal stress and side effects, Effects of Eliminating Maternal Antibody Interference and Simplifying Immunization Procedures

Active Publication Date: 2015-08-26
PU LIKE BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the common influence of maternal antibodies in piglets on pig farms, the effect of single vaccine immunity is not ideal, often resulting in immune failure
[0006] Not only that, to prevent the mixed infection of Mhp and Hps, a single vaccine needs at least four injections to prevent the disease, the cost is high, the operation procedure is cumbersome, the risk of infection is increased, and the stress response of the pigs is increased. Different vaccines are used It will cause immune interference and cause some problems such as immune failure of pig herds
[0007] The current technology cannot realize that one vaccine can immunize against the mixed infection caused by these two pathogens. Not only that, the combined vaccine is not only convenient, multi-effective, and low-cost, but also can reduce the number of vaccinations compared with a single vaccine. Avoid being unable to get full immunity due to missing seeds; in addition, most vaccines are not heat-resistant, and their production, transportation, storage, sales and even the entire use process must be carried out at a lower temperature, the so-called "cold chain". The interlocking cold chain operation is extremely expensive, making the cost of vaccines high, and the use of combined vaccines can greatly reduce the cost of cold chain operation, so it has significant advantages

Method used

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  • Vaccine composition, and preparation method and applications thereof
  • Vaccine composition, and preparation method and applications thereof
  • Vaccine composition, and preparation method and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1, preparation of mycoplasma swine pneumonia and Haemophilus parasuis dual inactivated vaccine

[0033] 1. Preparation of Mycoplasma hyopneumoniae antigen

[0034] After unsealing the freeze-dried strains, inoculate the liquid culture medium with 10% inoculum amount, shake and culture at 37°C for 3 to 7 days, and harvest when the pH value drops from 7.5 to 6.8. After pure inspection, they are used as first-class production seeds. Take the first-grade seeds and inoculate the liquid medium with 5% inoculum, shake and culture at 37°C for 3-7 days, harvest when the pH value drops from 7.5 to 6.8, and use them as secondary production seeds after pure inspection. The secondary seeds of Mycoplasma hyopneumoniae HN0613 strain were inoculated in liquid medium at 5% (v / v), cultured with shaking at 37°C for 3 to 7 days, and harvested when the pH value dropped from 7.5 to 6.8.

[0035] 2. Preparation of bacterial liquid of Haemophilus parasuis (type 4 JS strain and type...

Embodiment 2

[0062] Embodiment 2, this study is to evaluate the protective effect of the Mhp-Hps dual inactivated vaccine of trial production in resisting Mhp and Hps attack

[0063] 1. Test material

[0064] Vaccine 1 was prepared according to the method in Example 1 (Mhp antigen content was 7×10 8 CCU / toufen, Hps4 type JS strain antigen content is 2×10 9 CFU / portion, Hps5 type ZJ strain antigen content is 2×10 9 CFU / head) and vaccine 2 (Mhp antigen content is 4×10 8 CCU / toufen, Hps4 type JS strain antigen content is 2×10 9 CFU / portion, Hps5 type ZJ strain antigen content is 2×10 9 CFU / head serving).

[0065] 2. Design of animal experiments

[0066] 2.1 Vaccine Immunization

[0067] A total of 110 healthy susceptible piglets aged 0 to 3 days were selected. The specific implementation scheme is shown in Table 3, and the route of vaccination is neck intramuscular injection.

[0068] Table 4 Mhp-Hps dual vaccine immunization groups

[0069]

[0070]

[0071] 2.2 Mycoplasma hyo...

Embodiment 3

[0092] Example 3. This study is to evaluate the influence of maternal antibodies on the trial-manufactured Mhp-Hps dual combination vaccine 1 and the combination of commercially available Mycoplasma hyopneumoniae single vaccine and Haemophilus parasuis inactivated vaccine

[0093] 1. Test material

[0094] Vaccine 1 was prepared according to the method in Example 1 (Mhp antigen content was 7×10 8 CCU / toufen, Hps4 type JS strain antigen content is 2×10 9 CFU / portion, Hps5 type ZJ strain antigen content is 2×10 9 hyopneumoniae single vaccine (Rebex) and Haemophilus parasuis inactivated vaccine (produced by Huazhong Agricultural University, Wuhan Keqian Animal Biological Products Co., Ltd., and China Animal Husbandry Co., Ltd. ).

[0095] 2. Design of animal experiments

[0096] 2.1 Vaccine Immunization

[0097] A total of 55 healthy susceptible piglets aged 0 to 3 days were selected, and the Mhp antibody and Hps antibody monitoring were all positive, suggesting that the pig...

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Abstract

The invention provides a vaccine composition comprising mycoplasma hyopneumoniae antigen of an immunizing dose, haemophilus parasuis antigen of an immunizing dose, and a pharmaceutically acceptable carrier. The vaccine composition is capable of eliminating requirements on multiple vaccines and multiple-dose immunizing; immune interference of the mycoplasma hyopneumoniae antigen with the haemophilus parasuis antigen is not induced, synergistic effect is improved, maternal antibody interference can be eliminated by immunizing with the vaccine composition, and effect is more stable; and in addition, animal stress response and side effect caused by single dose immunizing of the vaccine composition are little, so that immunizing procedure is simplified, production cost, transportation cost, and prevention cost are reduced, and practicability is excellent.

Description

technical field [0001] The invention relates to a vaccine composition, a preparation method and application of the composition, and belongs to the field of veterinary biological products. Background technique [0002] Mycoplasma pneumonia of swine (MPS) is a chronic, contagious disease caused by Mycoplasma hyopneumoniae (Mhp). Porcine respiratory disease complex (PRDC) is one of the important pathogens. The disease has endangered the pig industry for a long time and is widely distributed in countries all over the world. The disease mainly harms fattening pigs, especially the growth rate of 14-20 week-old pigs is reduced, which reduces the feed conversion rate, and symptoms such as loss of appetite, fever, cough, and dyspnea can also occur. [0003] Haemophilus parasuis disease is multiple serositis and arthritis in pigs caused by Haemophilus parasuis (Hps), also known as Glasser's Disease. Haemophilus parasuis mainly harms weaned piglets aged 4 to 8 weeks, and can also in...

Claims

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Application Information

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IPC IPC(8): A61K39/02A61K39/102A61P31/04
Inventor 张许科孙进忠田克恭
Owner PU LIKE BIO ENG
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