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A kind of explant pretreatment method before the bud induction culture of rubber tree seedlings

An explant and bud induction technology, which is applied in the fields of botanical equipment and methods, horticultural methods, plant regeneration, etc., can solve the problem of high pollution rate, and achieve the effects of low pollution rate, good disinfection effect and low production cost.

Inactive Publication Date: 2017-07-11
RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a kind of explant pretreatment method before the bud induction culture of rubber tree seedlings, to overcome the problem of high pollution rate in its culture process, make up for the deficiencies in the prior art

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] The explant pretreatment method before the rubber tree seedling bushy bud induction culture described in embodiment 1 may further comprise the steps:

[0014] (1) Pre-disinfection of airtight plastic greenhouses: 10 days before the rubber tree seedlings are moved in, once every 5 days, the first time with 0.1% carbendazim, and the second time with 0.1% dyson The manganese zinc liquid is sprayed on the ground and surroundings of the plastic greenhouse, and the disinfection treatment time is in the afternoon, and the plastic greenhouse is sealed after spraying;

[0015] (2) Pre-disinfection of seedlings: Before the seedlings are moved into the plastic greenhouse, spray and disinfect their whole plants with carbendazim with a concentration of 0.1% or mancozeb liquid with a concentration of 0.1%;

[0016] (3) Regular disinfection of seedlings: move the pre-sterilized seedlings into plastic greenhouses for cultivation, and then alternately use carbendazim with a concentratio...

Embodiment 2

[0019] The explant pretreatment method before the rubber tree seedling bushy bud induction culture described in embodiment 2 may further comprise the steps:

[0020] (1) Pre-disinfection of airtight plastic greenhouses: 10 days before the rubber tree seedlings are moved in, once every 5 days, the first time with 0.1% carbendazim, and the second time with 0.1% dyson The manganese zinc liquid is sprayed on the ground and surroundings of the plastic greenhouse, and the disinfection treatment time is in the afternoon, and the plastic greenhouse is sealed after spraying;

[0021] (2) Pre-disinfection of seedlings: Before the seedlings are moved into the plastic greenhouse, spray and disinfect their whole plants with carbendazim with a concentration of 0.1% or mancozeb liquid with a concentration of 0.1%;

[0022] (3) Regular disinfection of seedlings: move the pre-sterilized seedlings into plastic greenhouses for cultivation, then spray seedling plants, ground and plastic greenhous...

Embodiment 3

[0025] The explant pretreatment method before the rubber tree seedling bushy bud induction culture described in embodiment 3 may further comprise the steps:

[0026] (1) Pre-disinfection of airtight plastic greenhouses: 10 days before the rubber tree seedlings are moved in, once every 5 days, the first time with 0.1% carbendazim, and the second time with 0.1% dyson The manganese zinc liquid is sprayed on the ground and surroundings of the plastic greenhouse, and the disinfection treatment time is in the afternoon, and the plastic greenhouse is sealed after spraying;

[0027] (2) Pre-disinfection of seedlings: Before the seedlings are moved into the plastic greenhouse, spray and disinfect their whole plants with carbendazim with a concentration of 0.1% or mancozeb liquid with a concentration of 0.1%;

[0028] (3) Regular disinfection of seedlings: move the pre-sterilized seedlings into a plastic greenhouse for cultivation, and then spray the seedling plants, the ground and the ...

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Abstract

The invention relates to a pretreatment method for explants of rubber tree seedlings before inducing clustered buds, which comprises the following steps: (1) pre-sterilizing the airtight plastic greenhouse; (2) pre-sterilizing the seedlings; (3) regularly disinfecting the seedlings; (4) Cut off new branches. The beneficial effects of the invention are: (1) simple operation and good disinfection effect; (2) sustainable operation and low production cost; (3) low pollution rate, the lowest being 2.7%.

Description

technical field [0001] The invention belongs to the tissue culture rapid propagation technology of rubber tree (Hevea brasiliensis) seedlings, in particular to an explant pretreatment method before inducing and cultivating clustered buds of rubber tree seedlings. Background technique [0002] At present, there are several types of vegetative propagation techniques for rubber tree planting materials, such as budding, cutting and in vitro micropropagation. Cluster bud induction technology is an important in vitro micropropagation technology, but rubber tree cluster bud induction technology is usually taken directly from budded stems grown in the field that are polluted by a large number of bacteria and systemic fungi, etc. , resulting in a high rate of tissue culture contamination, almost 100%, with very low failure or efficiency. Many scientific researchers use carbendazim, chlorothalonil, anti-virus alum, gentamicin, carbenicillin sodium, thiosporin, streptomycin, tetracycl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G1/00A01H4/00
Inventor 李娟林位夫周立军
Owner RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI