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Method for separating and purifying monomeric compound from Chinese mahonia leaves through high-speed counter-current chromatography

A technology of high-speed countercurrent chromatography and ten leaves of merit, which is applied in the field of natural medicinal chemistry, can solve the problems of large sample loss, long time consumption, and unsatisfactory repeat effect, and achieve the effect of good stability and easy operation

Active Publication Date: 2015-09-09
HUAIYIN TEACHERS COLLEGE
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Problems solved by technology

[0004] These components are usually subjected to repeated column chromatography, the operation is complicated, time-consuming, solvent consumption is large, and the sample loss is large, and the repeat effect is not ideal.

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  • Method for separating and purifying monomeric compound from Chinese mahonia leaves through high-speed counter-current chromatography
  • Method for separating and purifying monomeric compound from Chinese mahonia leaves through high-speed counter-current chromatography
  • Method for separating and purifying monomeric compound from Chinese mahonia leaves through high-speed counter-current chromatography

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Embodiment Construction

[0018] Take 5.0 kg of Shibagongong leaves that were naturally dried in the shade, crush them, and extract pure ethanol as solvent ultrasonically, the solid-to-liquid ratio was 1:20, the extraction time was 2 h, filtered, and the filter residue was treated twice; the filtrates were combined and concentrated under reduced pressure to obtain ten The crude extract of Dagong leaves; the crude extract was dispersed with water, extracted with n-hexane, dichloromethane, ethyl acetate and n-butanol respectively, the extract was filtered, concentrated under reduced pressure to obtain n-hexane, dichloromethane and ethyl acetate respectively Ester, n-butanol and water extraction sections, stored in a 4°C refrigerator for later use. Use n-hexane-methanol-ethyl acetate water with a volume ratio of 1:5:1:5 as the solvent system, the flow rate of the mobile phase (lower phase) is 2 mL / min, the rotation speed of the separation column is 900 rpm, and the upper phase is the stationary phase. HSC...

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Abstract

The invention discloses a method for separating and purifying monomeric compound from Chinese mahonia leaves through high-speed counter-current chromatography. The method comprises the following steps: drying Chinese mahonia leaves in the shade, smashing the dried dry Chinese mahonia leaves, adding an ethanol solution, carrying out supersonic extraction and filtering, conducting vacuum concentration on the obtained filtrate to obtain a Chinese mahonia leaf extractive, extracting the extractive with solvents of different polarities, separating the ethyl acetate extraction section with a high-speed counter-current chromatographic instrument, collecting corresponding peak components according to a chromatogram, and carrying out vacuum concentration and drying to obtain high-purity caffeotannic acid, quercetin-3-O-beta-D-glucoside, and isorhamnetin-3-O-beta-D-glucoside. The method has the advantages that the defects of complex operation, dead adsorption loss and low yield of the conventional preparation method are overcome; the efficiency is high; the operation is simple; the preparation quantity is large; the comprehensive cost is low; the popularization and use values are high.

Description

technical field [0001] The invention belongs to the field of natural medicinal chemistry, in particular to a rapid separation and purification of chlorogenic acid, quercetin-3-O-β-D-glucoside, isorhamnetin-3- O-β-D-glucoside method. Background technique [0002] Top Ten Contributions Mahonia bealei (Fort.) Carr.) has the effects of clearing heat and detoxifying, reducing inflammation and relieving pain, drying dampness and purging fire, clearing liver and improving eyesight. It is also widely used as Kudingcha in the provinces south of the Qinling Mountains. It is widely used among the people as an indication for dizziness, tinnitus, soreness of the waist and knees, damp-heat diarrhea, stomach fire and toothache, icteric hepatitis and other diseases. Modern pharmacological research has found that Shigongo has antibacterial, anti-inflammatory, anti-viral, anti-tumor, anti-proliferation and hypotensive activities, and is a medicinal plant with development value. [0003] ...

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Application Information

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IPC IPC(8): C07H1/08C07H17/07C07C69/732C07C67/48
Inventor 胡卫成王新风沈婷吴磊
Owner HUAIYIN TEACHERS COLLEGE
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