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Temperature-controlled liquid culture method capable of improving yield of ganoderma lucidum polysaccharides

A liquid culture, Ganoderma lucidum polysaccharide technology, applied in the field of fermentation, can solve the problems of low yield of mycelium and the like, and achieve the effects of simple method, good repeatability and increasing polysaccharide content

Inactive Publication Date: 2015-09-09
苏州葛家坞生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] It is generally believed that the suitable temperature for the submerged fermentation of Ganoderma lucidum is 25-30°C. Appropriate temperature conditions must be given during fermentation. When the temperature is higher than 30°C, the mycelium grows faster, but the mycelium yield is low, and it is easy to autolyze, and the color of the fermentation liquid is dark. Above 36°C, the mycelium of Ganoderma lucidum stops growing, and above 40°C, the mycelium dies soon

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Experimental strain: Taishan Red Ganoderma lucidum, purchased from the Edible Fungi Research Laboratory of an Academy of Agricultural Sciences.

[0026] Experimental process: strain → slant cultivation → seed cultivation → shake flask cultivation → harvest.

[0027] The formula of the medium for seed cultivation is: peptone 1.7%, glucose 2.2%, KH2P040.15%, MgS040.07%, ZnSO40.01%, rare earth element terbium (Tb) 0.01 μmol / L, medium pH 7.8-7.9.

[0028] The medium formula for shake flask culture is: peptone 1.6%, glucose 2.1%, KH2P040.12%, MgS040.05%, ZnSO40.01%, rare earth element terbium (Tb) 0.01μmol / , the pH of the medium is 7.9-8.0 .

[0029] Wherein the seed cultivation process and the medicine bottle cultivation process are:

[0030] A temperature-controlled liquid culture method for improving the yield of Ganoderma lucidum polysaccharide, comprising the following steps:

[0031] Liquid seed culture: Inoculate the slanted Ganoderma lucidum into the liquid seed m...

Embodiment 2

[0034] Experimental strains: Changbai Mountain Purple Ganoderma lucidum, purchased from the Edible Fungi Research Laboratory of an Academy of Agricultural Sciences.

[0035] Experimental process: strain → slant cultivation → seed cultivation → shake flask cultivation → harvest.

[0036] The formula of the medium for seed cultivation is: peptone 1.7%, glucose 2.2%, KH2P040.15%, MgS040.07%, ZnSO40.01%, rare earth element terbium (Tb) 0.01 μmol / L, medium pH 7.8-7.9.

[0037]The medium formula for shake flask culture is: peptone 1.6%, glucose 2.1%, KH2P040.12%, MgS040.05%, ZnSO40.01%, rare earth element terbium (Tb) 0.01μmol / , the pH of the medium is 7.9-8.0 .

[0038] Wherein the seed cultivation process and the medicine bottle cultivation process are:

[0039] A temperature-controlled liquid culture method for improving the yield of Ganoderma lucidum polysaccharide, comprising the following steps:

[0040] Liquid seed culture: Inoculate the slanted Ganoderma lucidum into the ...

Embodiment 3

[0043] Experimental strains: Ganoderma lucidum with thin caps from Diaoluoshan, Hainan, purchased from the Edible Fungi Research Laboratory of an Academy of Agricultural Sciences.

[0044] Experimental process: strain → slant cultivation → seed cultivation → shake flask cultivation → harvest.

[0045] The formula of the medium for seed cultivation is: peptone 1.7%, glucose 2.2%, KH2P040.15%, MgS040.07%, ZnSO40.01%, rare earth element terbium (Tb) 0.01 μmol / L, medium pH 7.8-7.9.

[0046] The medium formula for shake flask culture is: peptone 1.6%, glucose 2.1%, KH2P040.12%, MgS040.05%, ZnSO40.01%, rare earth element terbium (Tb) 0.01μmol / , the pH of the medium is 7.9-8.0 .

[0047] Wherein the seed cultivation process and the medicine bottle cultivation process are:

[0048] A temperature-controlled liquid culture method for improving the yield of Ganoderma lucidum polysaccharide, comprising the following steps:

[0049] Liquid seed culture: inoculate the ganoderma slant int...

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Abstract

The invention discloses a temperature-controlled liquid culture method capable of improving the yield of ganoderma lucidum polysaccharides and relates to the technical field of fermentation engineering. The temperature-controlled liquid culture comprises two steps of liquid seed culture and shake-flask culture, each step comprises three fermentation processes, and the rotation speed, the culture temperature and the culture medium components in each fermentation process are regulated and controlled to change the growth and development states of ganoderma lucidum mycelia so as to improve the yield and the quality of ganoderma lucidum polysaccharides. Meanwhile, the temperature-controlled liquid culture method is simple and has high economic benefits.

Description

Technical field: [0001] The invention relates to the technical field of fermentation, in particular to a liquid culture method for improving the yield of ganoderma lucidum liquid-fermented polysaccharide by regulating the culture temperature and the formula of the culture medium. Background technique [0002] Polysaccharides are polymers of aldose and ketose linked together by glycosidic bonds that exist in nature, and are indispensable components of all living organisms. Polysaccharides are abundant in seaweed, fungi and higher plants. As a biological effect regulator, polysaccharide is not only a non-specific immune enhancer, but also has multiple functions such as lowering blood sugar, lowering blood pressure, and lowering cholesterol. [0003] Ganoderma lucidum, also known as red ganoderma, wood ganoderma, and ganoderma grass, was called "auspicious grass" in ancient times. In terms of fungal classification, it belongs to Basidiomycetes, Polyporacea, Polyporaceae, and ...

Claims

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Application Information

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IPC IPC(8): C12Q3/00C12P19/04C12R1/645
Inventor 蔡军峰
Owner 苏州葛家坞生物科技有限公司
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