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Method for preparing TiO2/SiO2 composite film for photoinduced cell detachment

A composite film and cell technology, applied in liquid chemical plating, metal material coating process, coating, etc., can solve the problems of easy peeling off of the film, weak bonding force between the film and the substrate material, etc., and achieve low cost and good adsorption , The effect of simple process

Active Publication Date: 2015-09-09
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although titanium dioxide can become super-hydrophilic under certain ultraviolet light conditions, the binding force between titanium dioxide nano-films and substrate materials is weak, and the film is easy to fall off when culturing cells.

Method used

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  • Method for preparing TiO2/SiO2 composite film for photoinduced cell detachment
  • Method for preparing TiO2/SiO2 composite film for photoinduced cell detachment
  • Method for preparing TiO2/SiO2 composite film for photoinduced cell detachment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] (1) Mix TEOS and THF at a volume ratio of 1:5 and stir evenly to make A solution; mix A solution with HNO 3 According to the volume ratio of 6:0.1, mix and stir evenly to form B solution; mix C 2 h 5 OH, H 2 O is fully mixed according to the volume ratio of 1:5 to make C solution; under stirring conditions, add C solution dropwise to B solution at a speed of 1 s / drop, continue to stir for 1 hour after the dropwise addition, and then put it away from light. Sealed and aged under conditions for 2 days to obtain SiO 2 Sol.

[0018] (2) TiO 2 The water sol and THF are diluted and mixed according to the volume ratio of 1:3 and stirred evenly, and ultrasonicated for 0.5h to obtain the diluted TiO 2 Sol.

[0019] (3) Put the cleaned PS substrate on the turntable of the spin coater, and use a pipette gun to measure 20ul SiO 2 The sol was added dropwise on the substrate at a speed of 6000r / min, and 20ul of the above-mentioned TiO was measured within 2s after spin-coating....

Embodiment 2

[0021] (1) Mix TEOS and THF according to the volume ratio of 1:6 and stir evenly to make A solution; mix A solution with HNO 3 According to the volume ratio of 6:0.1, mix and stir evenly to form B solution; mix C 2 h 5 OH, H 2 O is fully mixed according to the volume ratio of 1:5 to make C solution. Under stirring conditions, add C solution dropwise to B solution at a speed of 2 s / drop. Sealed and aged under conditions for 2 days to obtain SiO 2 Sol.

[0022] (2) TiO 2 The water sol and THF were diluted and mixed according to the volume ratio of 1:4 and stirred evenly, and ultrasonicated for 30 minutes to obtain the diluted TiO 2 Sol.

[0023] (3) SiO 2 Sol and TiO 2 The sol was mixed and stirred evenly according to the molar ratio of 1:5, and ultrasonicated for 30 minutes. The cleaned PP substrate was placed on the turntable of the spin coater, and 20ul TiO2 was measured with a pipette gun. 2 / SiO 2 The mixed sol was added dropwise on the substrate, spin-coated at 4...

Embodiment 3

[0025] (1) Mix TEOS and THF according to the volume ratio of 1:7 and stir evenly to make A solution; mix A solution with HNO 3 According to the volume ratio of 6:0.2, mix and stir evenly, and make B solution; C 2 h 5 OH, H 2 O is fully mixed according to the volume ratio of 1:5 to form a C solution. Under stirring conditions, add the C solution dropwise to the B solution at a speed of 1 s / drop. Sealed and aged for 2 days under light conditions to obtain SiO 2 Sol.

[0026] (2) TiO2 The water sol and THF were diluted and mixed according to the volume ratio of 1:5 and stirred evenly, and ultrasonicated for 40 minutes to obtain the diluted TiO 2 Sol.

[0027] (3) Put the cleaned PET substrate on the turntable of the spin coater, and use a pipette gun to measure 20ul SiO 2 The sol was added dropwise on the substrate at a speed of 7000r / min, and 20ul of diluted TiO was measured within 2s after spin-coating. 2 The sol was added dropwise and spin-coated. The total spin-coating...

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Abstract

The invention discloses a method for preparing a TiO2 / SiO2 composite film for photoinduced cell detachment. The method comprises the following steps that a sol-gel method is adopted firstly, and tetraethoxysilane, absolute ethyl alcohol, tetrahydrofuran, nitric acid and deionized water are taken as raw materials, and SiO2 sol is prepared; then the tetrahydrofuran is used for diluting TiO2 hydrosol to obtain TiO2 sol; the TiO2 / SiO2 double-layer composite film is prepared on the surface of a substrate through a spin-coating method, and heat treatment is carried out on the composite film at the temperature of 55 DEG C to 70 DEG C to obtain the TiO2 / SiO2 composite film for photoinduced cell detachment. The method for preparing the TiO2 / SiO2 composite film is simple in technology, high-temperature treatment is not needed, the cost is low, the prepared film hasthe long super-hydrophilic property, the biocompatibility is good, and the method can be used for fields of photoinduced cell detachment, tissue engineering and the like.

Description

Technical field [0001] The present invention involves a composite film preparation method, especially a TIO for photococytic strife 2 / Sio 2 Preparation method of composite film. Background technique [0002] Titanium dioxide is a non -toxic and inorganic compound with very good biocompatibility and chemical stability. After a certain period of time after ultraviolet light, it has light response changes, which can be transformed into a super -intimate state.Studies have shown that by constructing TIO 2 / Sio 2 The double-layer composite film can achieve the self-cleaning function [zhao yue liu, et al. Sol-gel sio 2 / TIO 2 Bilayer Films with Self-found and Antireflection Properties.solar Energy Materials & Solar Cells 92 (2008) 1434-1438]. [0003] Studies have found that cells can be cultured on the titanium nano -dot film. After UV light 20min, it can form a good cell residual, the resilience can reach more than 90%, and enable the cells to maintain good activity. Therefore, it i...

Claims

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Application Information

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IPC IPC(8): C23C18/12
Inventor 程逵成智国翁文剑
Owner ZHEJIANG UNIV