Inactivated lactic acid bacterium liquid preparation and preparing method thereof

A technology of liquid preparations and live lactic acid bacteria, which is applied in the field of microecological preparations, can solve the problems of low recovery rate of active ingredients and high cost, and achieve the effects of reducing production costs, improving cost performance, and promoting animal growth

Active Publication Date: 2015-09-16
清远海贝生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In view of the above-mentioned problems of high cost of inactivated lactic acid bacteria preparations and low recovery rate of active ingredients, the purpose of the present invention is to provide a stable, efficient and low-cost inactivated lactic acid bacteria liquid preparation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1 inactivated Lactobacillus casei liquid preparation and its preparation

[0026] A liquid preparation of inactivated lactic acid bacteria, including 98% Lactobacillus casei fermentation broth, 1% glucosamine hydrochloride and 1% betaine hydrochloride, wherein the content of lactic acid bacteria cells is 10 9 individual / mL.

[0027] Its preparation method is as follows:

[0028] (1) Preparation of strains: configure MRS liquid medium, adjust the pH to 6.2, sterilize at 115°C for 20 minutes, cool to 37°C, and pick 2 strains from the slope of Lactobacillus casei with an inoculation loop in an ultra-clean workbench. Ring the lawn, then inoculate it into the liquid MRS medium, and culture it statically at 37°C for 16 hours to obtain the liquid seeds of Lactobacillus casei;

[0029] (2) Preparation of Lactobacillus casei fermentation broth: configure liquid MRS medium, adjust pH to 6.2, sterilize at 115°C for 20 minutes, cool to 37°C, insert Lactobacillus casei ...

Embodiment 2

[0032] Embodiment 2 Inactivated Pediococcus lactis liquid preparation and its preparation

[0033] A liquid preparation of inactivated lactic acid bacteria, including 97% Pediococcus pentosaceae fermentation broth, 3% betaine hydrochloride, wherein the content of lactic acid bacteria cells is 10 10 individual / mL.

[0034] Its preparation method is as follows:

[0035] (1) Preparation of strains: configure MRS liquid medium, adjust the pH to 6.3, sterilize at 115°C for 20 minutes, cool to 37°C, and pick from the slope of Pediococcus pentosaceae with an inoculation loop in the ultra-clean workbench 2 rings of bacterial lawn, then inoculated into liquid MRS medium, and then cultured at 37°C for 17 hours to obtain liquid seeds of Pediococcus pentosaceus;

[0036] (2) Preparation of Pediococcus pentosaceus fermentation broth: configure liquid MRS medium, adjust the pH to 6.2-6.4, sterilize at 115°C for 20 minutes, cool to 37°C, and insert the obtained product in step (1) accordin...

Embodiment 3

[0039] Embodiment 3 inactivated compound lactic acid bacteria liquid preparation and its preparation

[0040] A liquid preparation of inactivated lactic acid bacteria, including 96% compound lactic acid bacteria fermentation broth, 2% gamma-aminobutyric acid, 2% betaine hydrochloride, wherein the content of lactic acid bacteria cells is 10 9individual / mL.

[0041] Its preparation method is as follows:

[0042] (1) Preparation of strains: configure MRS liquid medium, adjust the pH to 6.4, sterilize at 115°C for 20 minutes, cool to 37°C, and pick 2 rings of bacteria on the slope inoculated with Lactobacillus plantarum in the ultra-clean workbench Moss, then inoculated into liquid MRS medium, and then cultured at 37°C for 18 hours to obtain liquid seeds of Lactobacillus plantarum, and in the same way to prepare liquid seeds of Lactobacillus casei, liquid seeds of Streptococcus faecalis and Lactobacillus acidophilus liquid seeds.

[0043] (2) Preparation of compound lactic acid...

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PUM

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Abstract

The invention provides a stable, efficient and low-cost inactivated lactic acid bacterium liquid preparation to solve the technical problems that existing inactivated lactic acid bacterium preparations are high in cost, and the effective element recovering rate is low. The acid additives such as Betaine hydrochloride, glucosamine hydrochloride and gamma-aminobutyric acid are used as inactivators, the cost of the inactivation method is far lower than that of other inactivation methods, and the liquid preparation is far better than thermal inactivated lactic acid bacterium preparations on the aspects of the biological activity such as the cell recovering rate, the adhering performance to cancer cells of human body and promoting of the growth of animals; the production cost is greatly reduced, and the cost performance of the inactivated lactic acid bacterium liquid preparation is improved.

Description

【Technical field】 [0001] The invention relates to a human or feeding microecological preparation, in particular to an inactivated lactic acid bacteria liquid preparation and a preparation method thereof, belonging to the field of microecological preparations. 【Background technique】 [0002] As people pay more and more attention to food safety and breeding environment, various disadvantages of antibiotic abuse in the breeding process have been widely concerned. Since the European Union completely banned antibiotics as feed additives, related substitutes have been extensively studied, and probiotics are one of the most important research directions. [0003] Microecological preparation is a product composed of beneficial microorganisms and their metabolites, which can improve the intestinal microecological balance of humans and animals, improve the immunity of humans and animals, and improve the health status of humans and animals. At present, microecological preparations hav...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/744A61K35/747A61K47/26A61K47/18A61P31/04A61P37/04A61P3/00A61P35/00
Inventor 张家学王蔚淼齐振雄
Owner 清远海贝生物技术有限公司
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