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Process for extracting total flavonoids from peanut shells

A technology of total flavonoids and peanut shells, applied in the biological field, can solve problems such as unfavorable industrial production, and achieve the effects of low raw material cost, low industrial energy consumption, and extreme industrial energy consumption

Inactive Publication Date: 2015-09-16
SHENYANG BEIYUAN MEDICINE CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to overcome the defect that the prior art is unfavorable to industrialized production, the present invention discloses a process for extracting total flavonoids from peanut shells which is cost-saving and easy to operate. Through qualitative identification and content determination, a product with high total flavonoid content was obtained, laying a good foundation for preparation research

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  • Process for extracting total flavonoids from peanut shells
  • Process for extracting total flavonoids from peanut shells
  • Process for extracting total flavonoids from peanut shells

Examples

Experimental program
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Effect test

Embodiment 1

[0015] The investigation of the extraction method is as follows: crush the peanut shell appropriately, weigh 10g, place it in a round bottom flask, add 10 times the concentration of 70% ethanol, use ultrasonic extraction method and reflux extraction method respectively, each method extracts 3 times, 1 hour each time. Filter, accurately draw 0.4mL filtrate and place it in a 10mL volumetric flask, add 2mL of 5% AlCl 3 solution, then add 3.2mL of 1mol / L CH3COOK solution, and finally dissolve with methanol and set the volume to the mark. Scanning was performed in the wavelength range of 200-600 nm according to UV spectrophotometry. The total flavonoid content was calculated according to the standard curve equation A=0.0894C-0.022, r=0.9998. Extraction rate of total flavonoids (total flavonoid content of extract / amount of medicinal material) and transfer rate (total flavonoid content of extract / total flavonoid content of medicinal material*100%) were used as indicators to evaluat...

Embodiment 2

[0020] In this paper, ethanol was used as the extraction solvent to extract the total flavonoids of peanut shells, which ensured the total flavonoids content and reduced the pollution of organic solvents.

Embodiment 3

[0022] The investigation of ethanol concentration is as follows: crush peanut shells properly, weigh 10g, place in a round bottom flask, add 10 times the concentration of ethanol solutions of 0%, 10%, 30%, 50%, 70%, 90%, and reflux Extract for 2 hours, filter, and measure the absorbance of the filtrate according to the method in Example 1. Then calculate the total flavonoid content according to the standard curve in Example 1. The optimal concentration of ethanol was selected based on the extraction rate and transfer rate of total flavonoids. The results are shown in Table 2

[0023] Table 2 Comparison of the extraction rate and transfer rate of total flavonoids under different ethanol concentrations

[0024] Ethanol concentration (%) 0 10 30 50 70 90 Extraction rate (mg / g) 1.03 1.26 1.59 1.73 2.34 1.98 Transfer rate (%) 28.22 34.52 43.56 47.40 64.11 54.25

[0025] It can be seen from the above table that when the concentration is 70...

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Abstract

The present invention discloses a novel and simple method for extracting total flavonoids from peanut shells. A crude extraction method comprises: carrying out crude extraction on the crushed peanut shell powder with ethanol, recovering ethanol, and evaporating the residual extraction solvent to obtain an extract. A refining process comprises: extracting by petroleum ether, extracting by ethyl acetate and refining by macroporous adsorptive resin. The adsorption conditions and elution conditions of the macroporous adsorptive resin are investigated. The extraction refining process is simple in operation and stable and reliable in results, and is applicable to industrialized production.

Description

technical field [0001] The invention belongs to the field of biotechnology and relates to a process for extracting total flavonoids from peanut shells. Background technique [0002] Peanut is an annual herbaceous plant of the leguminous family, which is widely planted all over the world. my country is a big producer of peanuts, with an annual output of more than 15 million tons; it is also the country with the largest output of agricultural waste in the world, with an annual output of about 650 million tons of agricultural waste. The comprehensive utilization of agricultural waste is a subject that needs to be solved in all countries in the world. Especially with the increasing shortage of natural resources and the sharp increase in the amount of waste, agricultural waste has attracted more and more attention. The comprehensive utilization of agricultural waste is a subject that needs to be solved in all countries in the world. Especially with the increasing shortage of n...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/48
Inventor 王淑君郝丹妮
Owner SHENYANG BEIYUAN MEDICINE CO LTD
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