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A method for quantitatively analyzing retinol and its precursors on trace blood dried blood filter paper

A technology for retinol and its precursors, which is applied in the field of quantitative analysis of retinol and its precursors on trace blood dried blood filter paper, can solve the problems of lack of extraction of retinol, etc., and achieve good linear relationship, high precision and sensitivity high effect

Inactive Publication Date: 2018-01-02
CHILDRENS HOSPITAL OF CHONGQING MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is still a lack of international and domestic methods for extracting retinol and its precursors from dried blood filter paper.

Method used

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  • A method for quantitatively analyzing retinol and its precursors on trace blood dried blood filter paper
  • A method for quantitatively analyzing retinol and its precursors on trace blood dried blood filter paper
  • A method for quantitatively analyzing retinol and its precursors on trace blood dried blood filter paper

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] (1) Materials and methods

[0050]

[0051] main reagent

Manufacturer

All-trans Retinol Standard

American Sigma Corporation

Retinyl Acetate Standards

American Sigma Corporation

Retinyl palmitoyl ester standard

American Sigma Corporation

β-carotene standard

American Sigma Corporation

3,5-di-tert-butyl-4-hydroxytoluene

American Sigma Corporation

Chloroform (HPLC grade)

American fisher company

Methanol (HPLC grade)

American fisher company

19, 19, 19, 20, 20, 20-d6-retinyl acetate

American CIL Corporation

[0052] Reagent preparation: retinol, retinyl acetate, retinyl palmitoyl ester standard dissolved in 0.1% (w / v) in the methanol solution, the β-carotene standard substance is dissolved in the dichloromethane containing BHT 0.1% (w / v), and the preparation concentration is respectively 5mg / ml, 2mg / ml, 2mg / ml, 5mg / ml The stock solution in ml is diluted 1000 times wit...

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Abstract

The invention provides a method for detecting retinol and its precursor, comprising the following steps: (1) extracting retinol and / or its precursor from a sample; (2) separating retinol and / or its precursor by HPLC ; (3) MS / MS quantitative detection; the sample is a dried blood filter paper specimen, and the extractant used is chloroform. The present invention uses filter paper as a carrier to absorb a small amount of blood to make a dry blood filter paper, which overcomes the inconvenience of taking too much blood when collecting venous blood when collecting serum to detect retinol in the past, poor compliance of the crowd, death of animal models after blood drawing, and inconvenient specimens. Difficulties such as distance transportation. The present invention has small matrix effect, good linear relationship in analysis within the concentration range of the standard curve, low detection limit, high sensitivity, good accuracy and high precision, and has correlation with the result of detecting serum retinol by HPLC method, At the same time, the content of retinol and its precursors in the sample is analyzed simultaneously, which provides a method for comprehensively evaluating the level of retinol in the body.

Description

technical field [0001] The invention relates to an analysis and detection method for retinol and its precursor in trace blood dried blood filter paper sheets. Background technique [0002] Retinol in the blood needs to be ingested from animal and plant foods, and its precursors: β-carotene, retinyl acetate and retinyl palmitoyl ester are transformed and formed in the intestinal mucosa and liver cells, and co-exist in the blood. Only by combining information on the content of retinol and its precursors can we fully measure the level of retinol in the body. [0003] At present, fluorescence, ultraviolet spectrophotometry and high performance liquid chromatography (High performance liquid chromatography, HPLC) are generally used to quantitatively analyze retinol in serum or plasma. The extraction steps of the first two methods are cumbersome, and the results are greatly affected by impurities; The complex components in serum can be separated, and its detection sensitivity and...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 李廷玉苗静琨邹琳余加林杨亭
Owner CHILDRENS HOSPITAL OF CHONGQING MEDICAL UNIV
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