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Detection method for Ardisia crispa herb or extract thereof

A detection method and technology for gold medicinal materials, applied in the field of medicine, can solve the problems such as the quantitative analysis method of 100 liang gold saponins, etc., and achieve the effects of scientific and reasonable quality control method, simple and fast measurement, and good linear relationship.

Inactive Publication Date: 2015-09-16
SOUTHWEST UNIVERSITY FOR NATIONALITIES
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Problems solved by technology

[0004] At present, the research hotspots of this genus mainly focus on the aspects of chemical composition, pharmacological action and clinical application, etc., and some people have also established the method for the content determination of petrin and saponin A in Bailiangjin medicinal material (Hou Jinling et al.HPLC -Study on the method of ESLD to determine the content of petracenin and saponin A in Yao medicine Zhuyefeng [J] Journal of Guangxi Medical University 2014, 31(1): 50-54), but no saponin B was found Quantitative analysis method

Method used

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  • Detection method for Ardisia crispa herb or extract thereof
  • Detection method for Ardisia crispa herb or extract thereof
  • Detection method for Ardisia crispa herb or extract thereof

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Embodiment Construction

[0021] 1 Instruments, reagents and samples

[0022] High performance liquid chromatography (US Waters 515 series); Alltech ELSD 2000 evaporative light scattering detector; preparative high performance liquid chromatography (US Beckman Coulter BECHMAN 125P type), including SYSTEM GOLD 125PSolvent Module and 166p Detector, etc. ; Milli-Q ultrapure water device (Milipore, USA); Microfuge 18 desktop centrifuge (Beckman Coulter Co., Ltd., USA); KQ-500DE ultrasonic cleaning machine (Kunshan Ultrasonic Instrument Co., Ltd., power 300W, frequency 40kHz) ; BP-210D electronic analytical balance (Sartorius, Germany, 1 / 100,000); filter device (Autoscience) and microporous membrane (0.45μm). Column: Alltech Altimma C 18 (250mm×4.5mm, 5μm); Waters Symmetry C 18 (150mm×4.5mm, 5μm); Phenomenex Gemini C 18 (150mm×4.5mm, 5μm); Preparative chromatographic column: BECHMAN UL TRAPREP C18 (21.1mm×150mm, 10μm); Protection column: phenomnex C 18 .

[0023] Bailiangjin saponin B self-made (meltin...

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Abstract

The present invention discloses a detection method for an Ardisia crispa herb or an extract thereof. According to the method, a high performance liquid chromatography-evaporative light scattering detection method (HPLC-ELSD) is used to determine the active component ardisiacrispin B content, such that the intrinsic quality of the Ardisia crispa herb or the extract thereof can be completely evaluated. According to the present invention, the linear relationship, the precision, the stability, the repeatability, the sample injection recovery rate, the tolerance and the like of the method meet the quantitative analysis requirements; and with the method, the ardisiacrispin B content of the Ardisia crispa herb or the extract thereof is firstly determined, such that the rapid and efficient analysis method is provided for the investigation of the Ardisia crispa herb main active ingredient content, and the method can further be referred by the new drug research and development.

Description

technical field [0001] The invention belongs to the technical field of medicine, and relates to the quality control of Chinese medicinal materials, in particular to a detection method for the medicinal material or its extract. Background technique [0002] Ardisia crispa (Thunb.) DC.A. is an evergreen shrub plant of the genus Ardisia Sw. in the family Myrsinaceae, distributed in Sichuan, Guizhou, Hunan, Hubei, Jiangxi, Zhejiang, Fujian , Guangdong and Guangxi. The whole plant is used as medicine, which has the effects of clearing throat, clearing throat, dissipating stasis, reducing swelling, relaxing tendons and activating blood circulation, and has the functions of clearing away heat and detoxification, promoting blood circulation and relaxing tendons, eliminating phlegm and relieving cough, and promoting pus and granulation. It is mostly used in Chinese folks to treat sore throat, Tonsillitis, nephritis, edema, bruises, rheumatic pain, white turbidity, bone tuberculosis,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
Inventor 张琦伍红饶开晴陈炼红
Owner SOUTHWEST UNIVERSITY FOR NATIONALITIES
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