The invention provides distiller yeast which is prepared from the following main raw materials: sticky rice, oriental stephania roots, grass roots, betel nuts, gerbera piloselloides, bee sugar flowers, honeycomb grass, bee sugar grass, chilli dish, ardisia crispa, illicium simonsii, lespedeza cuneata, cantonese hedyotis, good fruits, asparagus fern, chrysanthemum, ginseng, caulis piper wallichii, saruma henryi, lysimachia christinae, kudzu, maopaoci, liquorice, paris polyphylla, white hens and red wheat. The invention further discloses a preparation method of the distiller yeast. The distiller yeast which is prepared by adding the oriental stephania roots, the grass roots, the betel nuts, the gerbera piloselloides, the bee sugar flowers, the honeycomb grass, the bee sugar grass, the chilli dish, the ardisia crispa, the illicium simonsii, the lespedeza cuneata, the cantonese hedyotis, the good fruits, the asparagus fern, the chrysanthemum, the ginseng, the saruma henryi, the lysimachia christinae, the kudzu, the maopaoci, the liquorice, the paris polyphylla, the white hens and the red wheat under good effects of the caulis piper wallichii for tonifying Yang and the kidney, dispelling wind, dredging collaterals and expelling rheumatism and waist-leg weakness and which is prepared from pure natural raw materials is rich in nutrition and has no toxic or side effect.