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Method for extracting ardisiacrispin A

A kind of gold element, XAD-7 technology, applied in the direction of steroids, organic chemistry, etc., can solve the problems such as the industrialization method report of 100 liang gold element A, which is beneficial to large-scale production operations, large processing capacity, and high product purity. Effect

Inactive Publication Date: 2012-08-15
NANJING ZELANG MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there is no report on the industrialization method of preparing baicalin A in China

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Take 1 kg of golden root and crush it into coarse powder, put it into the extraction tank, add 60% ethanol, and conduct continuous countercurrent ultrasonic extraction at 50°C for 60 minutes, the ultrasonic frequency is 30KHz, concentrate the extract until it has no alcohol smell, dilute with water, filter, and put the filtrate on the D141 type macroporous resin column adsorption, first eluted with water, then eluted with 65% ethanol solution, collected the eluate, dried at low temperature to obtain crude product, dissolved in methanol, injected into the preparative high performance liquid chromatograph, 25% acetonitrile solution was Mobile phase, ultraviolet on-line detection, the detection wavelength is 205nm, high concentration fractions are collected, concentrated by rotary evaporation, and dried at low temperature to obtain 2.58g of lilacine A with a content of 97.7%.

Embodiment 2

[0014] Take 1 kg of golden root and crush it into coarse powder, put it into the extraction tank, add 80% ethanol, and conduct continuous countercurrent ultrasonic extraction at 50°C for 80 minutes, the ultrasonic frequency is 40KHz, the extract is concentrated until it has no alcohol smell, diluted with water, filtered, and the filtrate HPD300 type macroporous resin column adsorption, first eluted with water, then eluted with 60% ethanol solution, collected the eluate, dried at low temperature to obtain crude product, dissolved with methanol, injected into the preparative high performance liquid chromatograph, 40% acetonitrile solution was Mobile phase, ultraviolet on-line detection, the detection wavelength is 205nm, collect high-concentration fractions, concentrate by rotary evaporation, and dry at low temperature to obtain 2.75g of lyoglycin A with a content of 96.2%.

Embodiment 3

[0016] Take 1 kg of golden root and crush it into coarse powder, put it into the extraction tank, add 80% ethanol, and conduct continuous countercurrent ultrasonic extraction at 30°C for 30 minutes, the ultrasonic frequency is 50KHz, concentrate the extract until it has no alcohol smell, dilute with water, filter, and put the filtrate on the D101 type macroporous resin column adsorption, first eluted with water, then eluted with 50% ethanol solution, collected eluate, dried at low temperature to obtain crude product, dissolved in methanol, injected into preparative high-performance liquid chromatography, 30% acetonitrile solution was Mobile phase, ultraviolet on-line detection, the detection wavelength is 205nm, collect high-concentration fractions, concentrate by rotary evaporation, and dry at low temperature to obtain 2.63g of lilacin A with a content of 95.4%.

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PUM

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Abstract

The invention belongs to the field of extracting and preparing Chinese herbal medicine active constituents and relates to a method for extracting ardisiacrispin A. The method comprises the following steps of: based on ardisia crispa roots as a raw material, carrying out continuous countercurrent ultrasonic extraction; and adsorbing the extract on a macroporous resin column and performing preparative high-performance liquid chromatographic separation, and the like, thereby obtaining the ardisiacrispin A. The method is short in production period; the obtained product is high in purity; and the method is easy for industrial production.

Description

technical field [0001] The invention belongs to the field of extracting effective components of traditional Chinese medicines, and relates to a method for extracting lianggenin A. Background technique [0002] Ardisiacrispin A (Ardisiacrispin A), the molecular formula is C 52 h 84 o 22 , with a molecular weight of 1016.22, is a triterpenoid saponin compound, mainly derived from the root of Bailiangjin. Bailiangjin is the root and rhizome of the purple gold bovine plant Bailiangjin or its variants Dayebailiangjin and Xingshanbailiangjin. Unsmooth cough and expectoration, damp-heat jaundice, urinating pain, rheumatic arthralgia, bruises, furuncles, unknown swollen poison, snake bites and other symptoms. Pharmacological studies have shown that: Aminocin A has the effect of contracting the uterus. At 8 μg / ml, the contraction effect on the isolated rat uterus is equivalent to 85% of the contraction effect caused by 0.2 μg / ml acetylcholine; on cAMP phosphodiester Enzyme into ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J71/00
Inventor 刘东锋万冬梅
Owner NANJING ZELANG MEDICAL TECH
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