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A biocontrol agent for preventing and treating walnut base rot and its preparation method

A technology for walnut-based rot and biocontrol agents, which is applied in the directions of botanical equipment and methods, biocides, fungicides, etc., to achieve the effects of high-efficiency inhibition, easy cultivation, and simple and easy preparation.

Active Publication Date: 2017-08-25
XINJIANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the control of walnut base rot by antagonistic strains

Method used

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  • A biocontrol agent for preventing and treating walnut base rot and its preparation method
  • A biocontrol agent for preventing and treating walnut base rot and its preparation method
  • A biocontrol agent for preventing and treating walnut base rot and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0096] The acquisition of embodiment 1 bacterial strain

[0097] 1. Isolation of antagonistic strains

[0098] 1.1 Treatment of diseased specimens

[0099] Cut the diseased-healthy junction of walnut base rot disease specimens into 5mm tissue blocks, disinfect the tissue blocks with 75% ethanol for 30 seconds, take them out immediately and place them in 1% sodium hypochlorite for 90 seconds, and finally rinse them with sterile water for 3 times Use sterilized filter paper to absorb the moisture on the surface of the tissue block to obtain the sterilized tissue block.

[0100] 1.2 Isolated strains

[0101] The obtained sterilized tissue pieces were cultured in PDA medium, and observed once every 24 hours at a constant temperature of 28°C. When a bacteriostatic zone grew around the tissue piece, the bacterial colonies in the bacteriostatic zone were marked by the streaking method. Line to NA plate medium for purification to obtain a single colony, and finally store the obtain...

Embodiment 2

[0132] The fermentation culture of embodiment 2 bacterial strains

[0133] 1. Purification of pathogenic bacteria

[0134] Pick the mycelium of pathogenic bacteria stored in the test tube and place it in the center of the PDA plate, and incubate at a constant temperature of 28°C for 2-3 days in the dark for later use.

[0135] 2. Preparation of strain GY-11 seed solution and preparation of fermentation

[0136] The strain GY-11 was activated by streaking and placed on NA medium, and after 24 hours of constant temperature cultivation at 28°C, a single colony was picked and inoculated into a 250mL Erlenmeyer flask with 50mL of NB medium, and cultured on a shaker at 28°C and 180r / min 24h to make seed solution.

[0137]3. Screening of medium

[0138] Take 1mL of the seed solution of strain GY-11 and inoculate them into 250mL Erlenmeyer flasks containing 50mL of sterilized PDA culture solution, Gao’s No. 1 culture solution, corn flour culture solution and reference medium respec...

Embodiment 3

[0198] The preparation of embodiment 3 biocontrol agents

[0199] The biocontrol strain GY-11 with the strain preservation number of CGMCC No.10809 was shaken and cultured in NB medium with a pH of 7. The culture temperature was 25°C. After centrifugation at a speed of 180r / min for 24 hours, the precipitate Bacteria water diluted to GY-11 total concentration of live bacteria is 1.6×10 8 -3.8×10 10 CFU / mL of bacterial agent.

[0200] It should be noted that the medium can also use one of NA medium, NB medium, PDA medium, and LB medium; the culture temperature of the shaking culture is suitable for the present invention in the range of 20°C-40°C ; The rotational speed of the centrifugal treatment is applicable to the present invention in the range of 120r / min-220r / min; the initial pH value of the medium is applicable to the present invention in the range of 5-9.

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Abstract

The invention discloses a biocontrol agent for preventing and treating walnut base rot and a preparation method thereof, and relates to the field of biocontrol preparations. The total concentration of viable bacteria in the finished product of the biocontrol agent of the invention is 1.6×108-3.8×1010 CFU / mL biocontrol strain GY-11, its strain preservation number is CGMCC No.10809. The biocontrol agent of the present invention has no adverse effect on plant growth, has the effect of inhibiting the pathogenic bacteria of walnut base rot, and has an inhibitory rate of more than 80% on the pathogenic bacteria of walnut base rot, and has an efficient and significant effect on walnut base rot. The control effect has good application prospect and market value.

Description

technical field [0001] The invention relates to a biological control agent, in particular to a biological control agent mainly composed of Bacillus subtilis which antagonizes the pathogenic bacteria of walnut base rot and a preparation method thereof. Background technique [0002] Walnut (Juglans regia L.) is a deciduous tree and is an important economic tree species in my country. It has rich nutritional value and health care function, and can be used to produce edible oil and various foods. As the second largest fruit tree in Xinjiang, walnut has a cultivated area accounting for a quarter of the total area of ​​forest and fruit in Xinjiang. The walnut industry has become one of the important pillar industries for farmers in Xinjiang to increase their income. Walnut is suitable for a wide range of growth and is often planted in mountainous areas and rural areas. It is an important timber tree species and ecological tree species. In recent years, with the gradual expansion ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/00A01P1/00A01P3/00
Inventor 马荣朱银飞田呈明梁英梅游崇娟范鑫磊
Owner XINJIANG AGRI UNIV
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