Pretreatment method of phenolic components in three mountainous sage plants

A technology of alpine sage and phenolic acids, applied to plant/algae/fungi/moss components, drug combinations, plant raw materials, etc., to achieve the effect of low resin price, stable and controllable quality, and easy implementation

Inactive Publication Date: 2015-09-23
CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The above research reports mainly focused on the chemical constituents of Salvia gansi and Salvia villosa through complex methods such as column chromatography an...

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  • Pretreatment method of phenolic components in three mountainous sage plants
  • Pretreatment method of phenolic components in three mountainous sage plants

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0028] Example 1 The pretreatment method of phenolic acid components in three kinds of Alpine Salvia plants, comprising the following steps:

[0029] ⑴ Extraction:

[0030] 1.0 kg of the whole herb of Salvia villosa was dried in the shade and pulverized to obtain pulverized sage plants, and an ethanol solution with a volume fraction of 75% was added to the pulverized sage plants according to the solid-liquid ratio of 1kg:4L, After extracting at 60°C for 3 h, filter, repeat 3 times, and combine to obtain the filtrate; the filtrate was dried under reduced pressure to a paste, and the deep red extract was obtained.

[0031] Wherein: the conditions of decompression drying refer to a vacuum degree of 0.09 MPa and a temperature of 40°C.

[0032] ⑵Resin column chromatography enrichment:

[0033] Add 10 times of its mass to the deep red extract in a methanol solution with a volume fraction of 10% to dissolve, and after separation by a resin column, add an aqueous solution, a m...

Embodiment 2

[0041] Example 2 The pretreatment method of phenolic acid components in three kinds of Alpine Salvia plants, comprising the following steps:

[0042] ⑴ Extraction:

[0043] 5.0 kg of the whole sage herb was dried in the shade and crushed to obtain the crushed sage plant, which was added with an ethanol solution with a volume fraction of 95% according to the solid-liquid ratio of 1kg:8L, After extracting at 95°C for 1 h, filter, repeat once, and combine to obtain the filtrate; the filtrate was dried under reduced pressure to a paste, and the deep red extract was obtained.

[0044] Wherein: the conditions of decompression drying refer to a vacuum degree of 0.04 MPa and a temperature of 65°C.

[0045] ⑵Resin column chromatography enrichment:

[0046] Add 5 times its mass to the deep red extract in a methanol solution with a volume fraction of 30% to dissolve, and after separation by a resin column, use an aqueous solution, a methanol solution with a volume fraction of 50%...

Embodiment 3

[0054] Example 3 The pretreatment method of phenolic acid components in three kinds of Alpine Salvia plants, comprising the following steps:

[0055] ⑴ Extraction:

[0056] Dry 2.0 kg of the whole sage herb of Kangding in the shade and crush it to obtain the crushed sage plant. Add an ethanol solution with a volume fraction of 80% to the crushed sage plant according to the solid-liquid ratio of 1kg:6L, After extracting at 80°C for 2 h, filter, repeat twice, and combine to obtain the filtrate; the filtrate was dried under reduced pressure until it became a paste, and the deep red extract was obtained.

[0057] Wherein: the conditions of decompression drying refer to a vacuum degree of 0.06 MPa and a temperature of 50°C.

[0058] ⑵Resin column chromatography enrichment:

[0059] Add 8 times of its mass to the deep red extract in a methanol solution with a volume fraction of 20% to dissolve it, and separate it with a resin column, then use an aqueous solution, a methanol ...

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Abstract

The invention relates to a pretreatment method of phenolic components in three mountainous sage plants. The method comprises the following steps: (1) extracting: drying salvia roborowskii, salvia przewalskii or salvia prattii in shade, crushing, then extracting by alcohol, filtering and drying to obtain paste, i.e. deepred extract; (2) spectral enrichment by resin column: dissolving deepred extract into methanol solution, separating by virtue of the resin column, carrying out gradient elution for the deepred extract by adopting aqueous solution and methanol solution, and collecting 40% to 50% methanol elution substance; decompressing and drying the 70% to 80% methanol elution substance, and obtaining a phenolic acid component crude product; (3) spectrally removing impurities by adopting silicone column: separating and eluting the phenolic acid component crude product by virtue of silicone column spectrum, collecting a fraction with Rf value of 0.15 to 0.25, and decompressing and drying the fraction to obtain the light yellow powdered phenolic acid component. The process is simple, the mass implementation can be realized, the repeatability is good, and the quality is stable and controllable.

Description

technical field [0001] The invention relates to the technical fields of medicinal chemistry and biomedicine, in particular to a pretreatment method for phenolic acid components in three species of Alpine Salvia plants. Background technique [0002] Salvia ( Salvia L.) is the largest genus of Lamiaceae. There are three main sage species distributed on the Qinghai-Tibet Plateau: Salvia villosa ( Salvia roborowskii), Gansey sage ( Salvia przewalskii Maxim.) and Kangding sage ( Salvia prattii). Modern studies have shown that the chemical components in Salvia genus are closer to those of Salvia miltiorrhiza, which has better antioxidant activity. At present, the chemical components isolated and identified from Salvia genus mainly include phenolic acids and terpenes, among which phenolic acids are expected to become one of the index chemical components of this raw material, and also its main antioxidant active components. [0003] At present, it has been reported that the...

Claims

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Application Information

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IPC IPC(8): A61K36/537A61P39/06
Inventor 党军邵赟张莉王启兰陶燕铎梅丽娟文怀秀于瑞涛
Owner CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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