Preparation method of graphene oxide composite material mediated by carboxymethyl chitosan and modified by hyaluronic acid
A carboxymethyl chitosan and hyaluronic acid modification technology, which is applied in the field of preparation of graphene oxide composite materials, can solve problems such as potential application value obstacles, easy agglomeration, etc., and achieves improved biocompatibility, simple preparation method, To achieve the effect of controlled release
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Embodiment 1
[0034] (1) Add 20.7mg GO into 8mL distilled water, and disperse until uniform by ultrasonic. Then, 15.6 mg EDC·HCl and 7.8 mg NHS were added under the condition of magnetic stirring, and the stirring activation reaction was carried out for 3 h. In addition, 28.8 mg of CMC was formulated into a 5 mg / mL aqueous solution, and added dropwise to the above-mentioned activated GO dispersion under magnetic stirring conditions, and stirred and reacted at room temperature for 24 h. Finally, the reaction solution was transferred to a dialysis bag (MW = 100,000), and unreacted impurities and by-products were removed by dialysis. First, dialysis was performed 3 times with PBS buffer, 2 L each time, and then 6 times with ultrapure water. Finally, the samples were freeze-dried to obtain GO-CMC, which were sealed and stored in a refrigerator at 4 °C until use.
[0035] (2) Dissolve 99.3mg HA (MW=6,800) in 10mL distilled water, add 2.8mg EDC·HCl and 1.4mg NHS, stir and activate for 3h, and pr...
Embodiment 2
[0038](1) Add 49.8mg GO into 10mL distilled water, and disperse until uniform by ultrasonic. Then, 31.7 mg EDC·HCl and 15.8 mg NHS were added under the condition of magnetic stirring, and the stirring activation reaction was carried out for 3 h. In addition, 63.4 mg of CMC was formulated into a 5 mg / mL aqueous solution, and added dropwise to the above-mentioned activated GO dispersion under magnetic stirring conditions, and stirred and reacted at room temperature for 24 h. Finally, the reaction solution was transferred to a dialysis bag (MW = 100,000), and unreacted impurities and by-products were removed by dialysis. First, dialysis was performed 3 times with PBS buffer, 2 L each time, and then 6 times with ultrapure water. Finally, the samples were freeze-dried to obtain GO-CMC, which were sealed and stored in a refrigerator at 4 °C until use.
[0039] (2) Dissolve 327.1mg HA (MW=6,800) in 30mL distilled water, add 9.2mg EDC·HCl and 4.4mg NHS, stir and activate for 3h, and ...
Embodiment 3
[0042] (1) Add 80.3mg GO to 15mL distilled water, and disperse until uniform by ultrasonic. Then, 51.2 mg EDC·HCl and 25.8 mg NHS were added under the condition of magnetic stirring, and the stirring activation reaction was carried out for 3 h. In addition, 90 mg of CMC was prepared into a 5 mg / mL aqueous solution, and added dropwise to the above-mentioned activated GO dispersion under magnetic stirring conditions, and stirred and reacted at room temperature for 24 h. Finally, the reaction solution was transferred to a dialysis bag (MW = 100,000), and unreacted impurities and by-products were removed by dialysis. First, dialysis was performed 3 times with PBS buffer, 2 L each time, and then 6 times with ultrapure water. Finally, the samples were freeze-dried to obtain GO-CMC, which were sealed and stored in a refrigerator at 4 °C until use.
[0043] (2) Dissolve 401.3mg HA (MW=6,800) in 40mL distilled water, add 11.3mg EDC·HCl and 5.4mg NHS, stir and activate for 3h, and prep...
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