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Method and system for extracting and separating schaftoside from desmodium styracifolium

A technology for widening the bellflower and its extract, applied in the field of phytochemistry, can solve problems such as large market demand, and achieve the effects of short production time, simple process operation and high product yield

Active Publication Date: 2015-09-30
WUHAN KANGLE PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, since schaftoside is a reference substance required for pharmacopoeia standard testing, the market demand is relatively large

Method used

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  • Method and system for extracting and separating schaftoside from desmodium styracifolium
  • Method and system for extracting and separating schaftoside from desmodium styracifolium
  • Method and system for extracting and separating schaftoside from desmodium styracifolium

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Figure 5 Show the general flow process of the inventive method, comprise following:

[0031] 1. Raw material processing: select 45,000-50,000 parts by weight of the leguminous plant Desmodium glabra, cut into 5-10 cm sections, wash off the sediment with drinking water, drain, and feed.

[0032] 2. Ethanol extraction: 80% ethanol reflux extraction twice, the first time 12 times the amount for 2 hours, the second time 10 times the amount for 1.5 hours; combine the two alcohol extracts, recover the ethanol until it has no alcohol smell.

[0033] 3. Concentration under reduced pressure: add water to dilute the extract concentrate to 5 times the volume of the medicinal material; filter through a 200-mesh filter cloth; get the column liquid; pack the net product resin that has been treated with 95% ethanol in advance, and replace it with purified water; The upper column liquid is pumped into the column for adsorption, and the flow rate is controlled to be 0.5-1 times BV / h. ...

Embodiment 2

[0044] Select 45,000-50,000 parts by weight of the leguminous plant Desmodium glabrata, cut into 5-10cm sections, wash off the sediment with drinking water, drain and feed. Reflux extraction with 80% ethanol twice, the first time 12 times the amount for 2 hours, the second time 10 times the amount for 1.5 hours; combine the two alcohol extracts, recover the ethanol until it has no alcohol smell. Dilute the extract concentrate with water to 5 times the volume of the medicinal material; filter with a 200-mesh filter cloth; get the upper column liquid; pack the net resin that has been treated with 95% ethanol in advance, and replace it with purified water; pump the upper column liquid Put it into the column, absorb, control the flow rate of 0.5-1 times BV / h, after the adsorption is completed, let it stand for 2 hours; rinse with purified water, the elution flow rate is 3-4BV / h, and the elution volume is 10BV; then elute with 60% ethanol , the elution flow rate is 2-3BV / h, and the...

Embodiment 3

[0047] Select 45,000-50,000 parts by weight of the leguminous plant Desmodium glabrata, cut into 5-10cm sections, wash off the sediment with drinking water, drain and feed. Reflux extraction with 80% ethanol twice, the first time 12 times the amount for 2 hours, the second time 10 times the amount for 1.5 hours; combine the two alcohol extracts, recover the ethanol until it has no alcohol smell. Dilute the extract concentrate with water to 5 times the volume of the medicinal material; filter with a 200-mesh filter cloth; get the upper column liquid; pack the net resin that has been treated with 95% ethanol in advance, and replace it with purified water; pump the upper column liquid Put it into the column, absorb, control the flow rate of 0.5-1 times BV / h, after the adsorption is completed, let it stand for 2 hours; rinse with purified water, the elution flow rate is 3-4BV / h, and the elution volume is 10BV; then elute with 60% ethanol , the elution flow rate is 2-3BV / h, and the...

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Abstract

The invention provides a method for extracting and separating schaftoside from desmodium styracifolium. The method comprises the following steps that reflux extraction is performed on the desmodium styracifolium by utilizing ethyl alcohol to obtain a desmodium styracifolium extractive; multi-level chromatographic separation is performed on the extractive by utilizing alcohol systems, wherein first-level chromatographic separation is performed on the extractive by utilizing the first alcohol system to obtain a first separation part, second-level chromatographic separation is performed on the first separation part by utilizing the second alcohol system to obtain the schaftoside. The invention further provides a system for implementing the method. The schaftoside is separated and extracted from the desmodium styracifolium by utilizing the method and / or the system, the technology is easy to operate, the production time is short, the production yield is high, the purity is high, and the schaftoside with the purity more than 98 percent can be obtained.

Description

technical field [0001] The invention belongs to the technical field of phytochemistry, and relates to a method and system for extracting and separating monomeric compounds in plants. Specifically, the invention relates to a method and system for extracting and separating schaftosides from Desmodium sativa. Background technique [0002] Desmodium styracifolium (Osbeck) Merr. is a plant of the leguminous genus Desmodium styracifolium (Osbeck) Merr. Its medicinal part is the dry aerial part, and its main chemical components are flavonoids, saponins, polysaccharides, alkaloids and other compounds. It has the effects of clearing away heat and dehumidification, diuresis and treating stranguria. It is used for hot shower, sand shower, stone shower, astringent and painful urination, edema and oliguria, jaundice, red urine, and urinary calculi. [0003] Schaftoside, also known as 6-C-glucose-8-C-arabinose acetin, is a yellow powder; molecular formula: C 26 h 28 o 14 ;Molecular we...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D407/14
CPCC07D407/14
Inventor 王学海许勇李莉娥杨仲文冯芸余通尹海龙杨婷黄璐曹儒宾谢长
Owner WUHAN KANGLE PHARMA
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