Method for extracting high-purity total flavanone from stragalus membranaceus

A total flavonoid, high-purity technology, applied in pharmaceutical formulations, medical preparations containing active ingredients, plant raw materials, etc., to achieve the effects of improving extraction rate, short extraction time, and simple operation

Inactive Publication Date: 2015-10-07
兰捷
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to overcome the existing problems in the extraction method of total flavonoids in Radix Astragali, and provide a method for extracting high-purity total flavonoids from Ginkgo biloba

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A method for extracting high-purity total flavonoids from Astragalus, comprising the following steps:

[0027] (1) Dried 100kg of Astragalus membranaceus (the content of total flavonoids is 2kg) to make the water content 10%, and then crushed to 60 mesh to obtain Astragalus membranaceus powder;

[0028] (2) Add the above-mentioned astragalus powder into an ultrasonic stirrer, then add 550L of ethanol with a concentration of 80%, start the stirrer, and stir for 40min at a speed of 300r / min;

[0029] (3) Then transfer the mixed solution to the reflux device, reflux and extract 3 times, reflux for 0.5h each time, and combine the extracts;

[0030] (4) Concentrate the above extract under reduced pressure for 2 hours at a temperature of 50°C and a pressure of 0.08Mpa to obtain a concentrated solution, then adjust the pH of the concentrated solution to 2 with dilute hydrochloric acid with a concentration of 5%, and remove the precipitate After filtering, drying at a temper...

Embodiment 2

[0035] A method for extracting high-purity total flavonoids from Astragalus, comprising the following steps:

[0036] (1) Dried 100kg of Astragalus membranaceus (the content of total flavonoids is 2kg) to make the water content 10%, and then crushed to 60 mesh to obtain Astragalus membranaceus powder;

[0037] (2) Add the above-mentioned astragalus powder into an ultrasonic stirrer, then add 600L of ethanol with a concentration of 85%, start the stirrer, and stir for 30min at a speed of 400r / min;

[0038] (3) Transfer the mixed solution to the reflux device, reflux and extract 3 times, reflux for 1 hour each time, and combine the extracts;

[0039] (4) Concentrate the above extract under reduced pressure for 2 hours at a temperature of 55°C and a pressure of 0.09Mpa to obtain a concentrated solution, then adjust the pH of the concentrated solution to 3 with 5% dilute hydrochloric acid, and remove the precipitate After filtering, drying at a temperature of 55°C for 1.5 hours...

Embodiment 3

[0044] A method for extracting high-purity total flavonoids from Astragalus, comprising the following steps:

[0045] (1) Dried 100kg of Astragalus membranaceus (the content of total flavonoids is 1.98kg) in the sun to make the water content 13%, and then crushed to 80 mesh to obtain Astragalus membranaceus powder;

[0046] (2) Add the above-mentioned astragalus powder into an ultrasonic stirrer, then add 550L of ethanol with a concentration of 90%, start the stirrer, and stir for 30min at a speed of 500r / min;

[0047] (3) Then transfer the mixed solution to the reflux device, reflux extraction 4 times, reflux each time for 0.5h, and combine the extracts;

[0048] (4) Concentrate the above extract under reduced pressure for 1.5 hours at a temperature of 60°C and a pressure of 0.08Mpa to obtain a concentrated solution, and then adjust the pH of the concentrated solution to 2 with dilute hydrochloric acid with a concentration of 8%. After the precipitate is filtered, it is dr...

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Abstract

The invention discloses a method for extracting high-purity total flavanone from stragalus membranaceus. The method comprises the following steps that the stragalus membranaceus is smashed after being dried, then ethyl alcohol with the weight ratio of 1:4-6 is added into the smashed stragalus membranaceus to be stirred in an ultrasonic stirrer for 30-40 min, and a mixed solution is obtained; then the mixed solution is transferred to a reflux device for reflux extraction, and an extracting solution is obtained; diluted hydrochloric acid is used to adjust the pH value of the extracting solution to be 2-3 through decompressing condensing, and a total flavanone crude product is obtained after drying; then silica gelcolumn chromatography is conducted on the crude product, the dried solid substance is dissolved by methyl alcohol, and the total flavanone can be obtained through recrystallizing, filtering and drying. Be means of the method for extracting the high-purity total flavanone from the stragalus membranaceus, the extraction ratio of the total flavanone reaches 80%, and the purity reaches above 90%. The method for extracting the total flavanone from the stragalus membranaceus has the advantages that the operation is simple, the energy consumption is low, the extracting time is short, the extracting ratio is high, the purity is high, and the cost is low. The industrial production is easy to achieve.

Description

technical field [0001] The invention relates to the field of extracting effective components of Chinese herbal medicines, in particular to a method for extracting high-purity total flavonoids from Astragalus membranaceus. Background technique [0002] Astragalus is the root of a perennial herbaceous plant in the leguminous family. It is sweet in taste and warm in nature, and can be used as a tonic. The main medicinal ingredients in Astragalus membranaceus are flavonoids, and there are more than 30 kinds of flavonoids, mainly including quercetin, rutin, pistil isoflavones, and formononetin. The content of total flavonoids in Astragalus membranaceus is 1-3%. The active ingredients in Astragalus membranaceus total flavonoids have significant anti-hepatotoxicity, anti-inflammatory, antibacterial and cardiovascular protection effects, and also have anti-oxidation functions, which are widely used in the field of cosmetics . [0003] The total flavonoids in Astragalus membranaceu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/481A61K8/97
Inventor 兰捷
Owner 兰捷
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