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Applications of targeted HOTAIR-inhibiting small RNA in preparation of anti-prostate cancer drugs

A technology of anti-prostate cancer and medicine, applied in the biological field, achieves good inhibitory effect, simple operation and low cost

Inactive Publication Date: 2015-10-14
ZHEJIANG UNIV
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Problems solved by technology

At present, the research on HOTAIR in prostate cancer is still blank in China. This study proves that the expression of HOTAIR is up-regulated in prostate cancer cells, indicating that the occurrence and development of prostate cancer may be related to HOTAIR.

Method used

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  • Applications of targeted HOTAIR-inhibiting small RNA in preparation of anti-prostate cancer drugs
  • Applications of targeted HOTAIR-inhibiting small RNA in preparation of anti-prostate cancer drugs
  • Applications of targeted HOTAIR-inhibiting small RNA in preparation of anti-prostate cancer drugs

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Embodiment 1

[0015] Embodiment 1: siRNA down-regulates the expression of HOTAIR and inhibits the biological function of prostate cancer cells, embodiment:

[0016] 1. Cell lines: human normal prostate epithelial cells RWPE-1, prostate cancer cell line PC3, and prostate cancer cell line DU145 (all from Shanghai Institute of Cells, Chinese Academy of Sciences).

[0017] 2. siRNA synthesis: siRNA was chemically synthesized by Shanghai Jikai Biotechnology Co., Ltd.

[0018] 3. Experimental grouping: siRNA group (si-HOTAIR1, si-HOTAIR2), control group (si-Control)

[0019] 4. Cell culture: use RPMI-1640 medium for the above three types of cells, then add 10% fetal bovine serum, 100U / ml penicillin and 100mg / ml streptomycin to the RPMI-1640 medium, place at 37°C, contain 5 %CO 2 cultured in a constant temperature incubator.

[0020] 5. qRT-PCR detection: qRT-PCR technology was used to detect the expression levels of HOTAIR in the three cell lines. Total RNA in cells was extracted using Trizol...

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Abstract

The present invention discloses applications of targeted HOTAIR-inhibiting small RNA in preparation of anti-prostate cancer drugs. According to the present invention, the DNA sequence encoding HOTAIR gene is located between the gene HOXC11 and the gene HOXC12 of the chromosome 12, HOTAIR is a spliced polynucleotide transcription product having a length of 2158 nt, and the sequences encoding HOTAIR comprises five short exons and a long exon; the small RNA comprises a variety of siRNA sequences, wherein the small RNA can downregulate the expression of the HOTAIR gene in prostate cancer cells in a targeted manner so as to induce cell cycle arrest and inhibits cell invasion and migration ability, such that the small RNA can be used for the preparation of the anti-prostate cancer drugs; the siRNA of the present invention has characteristics of simple preparation, low cost, less consumption and exact inhibition effect, wherein the good inhibition effect can be achieved only with the 50 nM transfection concentration; and the siRNA inhibits the gene expression while the integrity of the genome can not be damaged.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to the application of small RNA targeting and inhibiting HOTAIR in the preparation of anti-prostate cancer drugs. Background technique [0002] In recent years, some studies have shown that non-coding RNAs (ncRNAs) are related to a variety of cellular pathways, especially tumor-related proliferation, apoptosis, and metastasis. MicroRNA is one of the most studied non-coding RNAs with about 22 bases, which can play a regulatory role at the transcriptional and post-transcriptional levels. Long non-coding RNA (LncRNA) is another type of non-coding RNA that has been studied more. LncRNA is a class of non-coding RNA with a length greater than 200 bases, which is involved in almost all processes related to mRNA, from transcription to mRNA splicing, RNA degradation and translation. Dysregulation of lncRNAs is closely related to human diseases, especially tumors, which may affect epigenetic info...

Claims

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Application Information

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IPC IPC(8): A61K48/00A61K31/7088A61P35/00
Inventor 金晓东朱意虞日考纪阿林姚晓霖方家杰
Owner ZHEJIANG UNIV
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