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A kind of extraction method of paeoniflorin monomer

An extraction method, the technology of paeoniflorin, applied in the field of extraction of paeoniflorin monomers, can solve the problems of large solvent consumption, decreased product yield, and long process, and achieve the effects of high product purity, easy operation, and reduced loss

Active Publication Date: 2018-06-01
JIANGSU TIANSHENG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The overall defects of these extraction methods and purification methods are that there are many impurities in paeoniflorin, large solvent consumption, large residue, long process, difficult purification in the later stage, and a small part of paeoniflorin is lost during purification of macroporous adsorption resin, which reduces the yield of the product. , it is difficult to achieve a pure product with a content of more than 98% by later purification methods

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1) Test steps:

[0022] (1) Get 100g of Radix Paeoniae Rubra medicinal material and pulverize it, pass through a 60-mesh sieve, and place it in an acidic aqueous solution with a pH of 3-6 as a solute for sulfuric acid for 1 hour. The volume ratio of the medicinal material to the acidic aqueous solution is 1:10. , take out the medicinal material and extract it with an ultrasonic microwave combination equipment, adjust the ultrasonic power to 600-800W, and the microwave power to 600-1000W; the extraction time is 40min, and an extract is obtained after pressing; The acidic aqueous solution of 3-6 was extracted for 40 minutes, and the secondary extract was obtained after filtration;

[0023] (2) Mix the primary extract and the secondary extract evenly, extract with 1 times the volume of organic solvent ethyl acetate, the number of extractions is twice, take the lower layer extract obtained twice, combine and mix, and concentrate under reduced pressure Obtain the total gluc...

Embodiment 2

[0028] 1) Test steps:

[0029] (1) Take 100g of Radix Paeoniae Rubra medicinal material and pulverize it, put it through a 60-mesh sieve and place it in an acidic aqueous solution with a pH of 3-6 as a solute and soak it for 2 hours. The volume ratio of the medicinal material to the acidic aqueous solution is 1:10. After the soaking is completed, Take out the medicinal materials and extract them with ultrasonic and microwave combination equipment, adjust the ultrasonic power to 600-800W, and the microwave power to 600-1000W; the extraction time is 40min, and an extract is obtained after pressure filtration; add 5 times the volume of pH to 3 The acidic aqueous solution of -6 was extracted for 40 minutes, and the secondary extract was obtained after filtration;

[0030] (2) Mix the primary extract and the secondary extract evenly, extract with 2 times the volume of organic solvent sherwood oil, the extraction times are twice, take the lower layer extract obtained twice, combine ...

Embodiment 3

[0035] 1) Test steps:

[0036] (1) Take 100g of Radix Paeoniae Rubra medicinal material and pulverize it, put it through a 60-mesh sieve and place it in an acidic aqueous solution with a pH of 3-6 as a solute and soak it for 2 hours. The volume ratio of the medicinal material to the acidic aqueous solution is 1:10. After the soaking is completed, Take out the medicinal materials and extract them with ultrasonic and microwave combination equipment, adjust the ultrasonic power to 600-800W, and the microwave power to 600-1000W; the extraction time is 40min, and an extract is obtained after pressure filtration; add 5 times the volume of pH to 3 The acidic aqueous solution of -6 was extracted for 40 minutes, and the secondary extract was obtained after filtration;

[0037] (2) Mix the primary extract and the secondary extract evenly, extract with 3 times the volume of organic solvent ethyl acetate, the number of extractions is twice, take the lower layer extract obtained twice and ...

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Abstract

The invention discloses a method for extracting paeoniflorin monomers, which uses an acidic aqueous solution as a solvent and combines ultrasonic-microwave combined extraction technology to extract paeoniflorin monomers, which is comparable to traditional column chromatography or macroporous resin adsorption methods. It has the following obvious advantages: a) has the advantages of fast, thorough and high efficiency, can shorten the production cycle and improve the extraction rate of paeoniflorin in the production process; b) reduces the loss in the separation process, and is easy to operate and industrialized production c ) is purified by preparative chromatography, which has the characteristics of low loss and high product purity; the semi-preparative chromatographic conditions can be scaled up year-on-year, and is suitable for industrial-grade biomedical liquid-phase preparative chromatography devices to realize the industrial production of high-purity paeoniflorin.

Description

technical field [0001] The invention relates to the field of extraction and separation of plants, in particular to a method for extracting paeoniflorin monomers. Background technique [0002] Radix Paeoniae Rubra is the dry root of Paeonia lactiflora Pall. or Chuanchi, aeonia veitchiiLynch. Bitter in the mouth; slightly cold in nature. It has the effects of clearing heat and cooling blood; promoting blood circulation and removing blood stasis. Mainly warm and poisonous spots; vomiting blood and epistaxis; intestinal wind and blood; conjunctival congestion and pain; carbuncle sores; amenorrhea; Modern pharmacological experiments have proved that it has the effect of dilating coronary arteries and increasing blood flow; it can be used for chest tightness, angina pectoris, and hyperlipidemia of coronary heart disease. [0003] Paeoniflorin is the main active ingredient of Radix Paeoniae Rubra, which has the effects of dilating blood vessels, analgesia and sedation, anti-infl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H17/04C07H1/08
Inventor 季浩阚建伟徐娟
Owner JIANGSU TIANSHENG PHARMA