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Preparation method of high-strength light sensitive hydrogel

A high-intensity light and hydrogel technology, applied in the field of high-strength hydrogel preparation, can solve problems such as gel damage and weak hydrogel mechanical properties, and achieve high water absorption, significant photosensitivity, and easy long-term Effects of preservation and long-distance transport

Inactive Publication Date: 2015-10-21
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, for traditional hydrogels, the dilution effect of water molecules in the gel on the polymer network, the disordered distribution of cross-linking points and the length of chain links, and the inhomogeneity of the cross-linking network during the gelation process cause The mechanical properties of hydrogel are very weak, it is difficult to be used as a soft tissue replacement under high load, and even the touch of tweezers and other utensils during the movement can easily damage the gel, thus limiting its application as a biomaterial

Method used

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  • Preparation method of high-strength light sensitive hydrogel
  • Preparation method of high-strength light sensitive hydrogel
  • Preparation method of high-strength light sensitive hydrogel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Monomer oligoethylene glycol methacrylate (6 mg), 2-vinyl-4,6-diamino-1,3,5-triazine (6 mg), SP (0.6 mg) and crosslinker were polymerized Ethylene glycol diacrylate (8.4 mg) was added to a 1.5 ml centrifuge tube, and after dissolving the monomer and cross-linking agent with 210 μl of DMSO, photoinitiator Irgacure 2959 (0.5 mg) was added. The solvent containing monomer, crosslinking agent and initiator was injected into a closed mold, and the mold was irradiated in a UV curing oven for 30 min to initiate free radical polymerization. Then the mold was opened to take out the gel, rinsed with deionized water for several times and soaked for 7 days, and the deionized water was replaced every 12 h.

[0033] Cell culture and desorption on the gel surface: The obtained gel was immersed in medical alcohol for 4 hours and then placed in a 96-well plate, and 200 μl of DMEM medium was added to replace the medical alcohol. The L929 cell suspension was added to the culture plate wit...

Embodiment 2

[0036] Monomer oligoethylene glycol methacrylate (6 mg), 2-vinyl-4,6-diamino-1,3,5-triazine (12 mg), SP (0.9 mg) and crosslinker were polymerized Ethylene glycol diacrylate (12.6 mg) was added to a 1.5 ml centrifuge tube, and after dissolving the monomer and cross-linking agent with 315 μl of DMSO, photoinitiator Irgacure 2959 (0.7 mg) was added. The solvent containing monomer, crosslinking agent and initiator was injected into a closed mold, and the mold was irradiated in a UV curing oven for 30 min to initiate free radical polymerization. Then the mold was opened to take out the gel, rinsed with deionized water for several times and soaked for 7 days, and the deionized water was replaced every 12 h.

[0037] Cell culture and detachment on the gel surface: The obtained gel was immersed in medical alcohol for 4 hours and then placed in a 96-well plate, and 200 μl of DMEM medium was added to replace the medical alcohol. The L929 cell suspension was added to the culture plate w...

Embodiment 3

[0040] Monomer oligoethylene glycol methacrylate (6 mg), 2-vinyl-4,6-diamino-1,3,5-triazine (18 mg), SP (1.2 mg) and crosslinker were polymerized Ethylene glycol diacrylate (16.8 mg) was added to a 1.5 ml centrifuge tube, and after dissolving the monomer and cross-linking agent with 420 μl of DMSO, photoinitiator Irgacure 2959 (0.9 mg) was added. The solvent containing monomer, crosslinking agent and initiator was injected into a closed mold, and the mold was irradiated in a UV curing oven for 30 min to initiate free radical polymerization. Then the mold was opened to take out the gel, rinsed with deionized water for several times and soaked for 7 days, and the deionized water was replaced every 12 h.

[0041] Cell culture and detachment on the gel surface: The obtained gel was immersed in medical alcohol for 4 hours and then placed in a 96-well plate, and 200 μl of DMEM medium was added to replace the medical alcohol. The L929 cell suspension was added to the culture plate w...

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Abstract

The invention discloses high-strength photosensitive hydrogel as well as a preparation method and application thereof. The high-strength photosensitive hydrogel is formed by free radical polymerization copolymerization of polyethylene glycol methacrylic ester, 2-vinyl-4,6-diamino-1,3,5-triazine and a double-bond-containing monomer with a spiropyrane structure; the three monomers and an initiator are dissolved in a solvent, unsaturated bonds on the molecules of the monomers are initiated by the initiator, and the hydrogel is prepared through free radical polymerization reaction. The photosensitive hydrogel disclosed by the invention has higher stretching resistance and compression resistance, and has good biocompatibility. Moreover, the hydrogel has the capability of adsorbing DNA (Deoxyribonucleic Acid) transfection cells as well as adjusting attaching and un-attaching behaviors of the cells by illumination.

Description

[0001] The present application is a divisional application of the parent application "High-intensity light-sensitive hydrogel and its preparation method and application", the parent application number is 201310473943.1, and the filing date is October 11, 2013. technical field [0002] The technical field of polymer hydrogels of the present invention, more specifically, relates to the preparation of high-strength hydrogels with cell desorption ability and transgenic efficacy, namely polyethylene glycol methacrylate-co-2-vinyl- Preparation and application of 4,6-diamino-1,3,5-triazine-co-copolymer containing spiropyran monomer (PEGMA-co-PVDT-co-PSP) hydrogel. Background technique [0003] Polymer hydrogel is a kind of three-dimensional network polymer with high water content and can maintain the original structure without being dissolved after water absorption. It has important application value in the field of medical engineering. However, for traditional hydrogels, the dilu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08F283/06C08F226/06C08F224/00
Inventor 刘文广王宁张金龙孙亮王朋延
Owner TIANJIN UNIV
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