Method for detecting sterility of photo-thermo-sensitive genic male sterile rice according to multiphase and multi-sample method

A technology for photothermo-sensitive nuclear sterility and light detection, which is applied in the fields of botanical equipment and methods, measuring devices, plant genetic improvement, etc. , the effect of improving the accuracy

Active Publication Date: 2015-11-04
RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

For example, in the technical specifications for the two-line hybrid rice seed production system promulgated according to the national standard of the People's Republic of China (GB/T 29371.1-2012), during the treatment of sterile line materials, indica rice is the main stem and young panicle differentiation enters the end of stage IV, and japonica rice is the main When the differentiation of stems and young panicles enters the end of the third stage, they are transferred to an artificial climate box for treatment. However, this treatment method can onl

Method used

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  • Method for detecting sterility of photo-thermo-sensitive genic male sterile rice according to multiphase and multi-sample method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The specific operation steps of a method for detecting the sterility of photothermosensitive genic sterile rice by a multi-stage multi-sample method are as follows:

[0029] (1) Sow Xin'an S, the photothermosensitive genital sterile rice seed to be treated, every 10 days on May 11, May 21, June 1 and June 11, 2014. A total of 4 sows were sown. Expect;

[0030] (2) After germination, sow in the field, and transplant in the field at the age of 5-6 leaf seedlings. Observe the progress of the ear growth of the first phase material, and 15 days before the treatment, select 5 uniform and robust plants for each sowing period, and transplant them in 1 pot respectively, to be treated, and to be managed by conventional techniques;

[0031] (3) At 19:00 on July 30th, 20% of the plants sown on May 11th will enter the leaf pillow flat period, and the 4 Plants that are expected to be sowed are placed together in a constant temperature cold water pool at 23.5°C for 6 consecutive days ...

Embodiment 2

[0036] The specific operation steps of a method for detecting the sterility of photothermosensitive genic sterile rice by a multi-stage multi-sample method are as follows:

[0037] (1) Sow P88S seeds of photothermosensitive genic male sterile rice to be treated every 10 days on May 11, May 21, June 1 and June 11, 2014, for a total of 4 sowing periods ;

[0038] (2) After germination, sow in the field, and transplant in the field at the age of 5-6 leaf seedlings. Observe the progress of the ear growth of the first phase material, and 15 days before the treatment, select 5 uniform and robust plants for each sowing period, and transplant them in 1 pot respectively, to be treated, and to be managed by conventional techniques;

[0039] (3) At 19:00 on July 30th, 10% of the plants sown on May 11th will enter the leaf pillow flat period, and the 4 The plants to be treated at the sowing stage were placed together in a constant temperature cold water pool at 23.5°C for continuous cul...

Embodiment 3

[0044] The specific operation steps of a method for detecting the sterility of photothermosensitive genic sterile rice by a multi-stage multi-sample method are as follows:

[0045] (1) Sow 2301S seeds of photothermosensitive genetic male sterile rice to be treated on May 11, May 21, June 1 and June 11, 2014, respectively, every 10 days, for a total of 4 sowing periods ;

[0046] (2) After germination, sow in the field, and transplant in the field at the age of 5-6 leaf seedlings. Observe the progress of the ear growth of the first phase material, and 15 days before the treatment, select 5 uniform and robust plants for each sowing period, and transplant them in 1 pot respectively, to be treated, and to be managed by conventional techniques;

[0047] (3) At 17:00 on July 26, 15% of the plants sown on May 21 entered the leaf pillow flat period, and the 3 plants to be treated on May 21, June 1 and June 11 were sown Place them together in a constant temperature cold water pool at...

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Abstract

The invention discloses a method for detecting sterility of photo-thermo-sensitive genic male sterile rice according to a multiphase and multi-sample method. The method comprises the steps of sowing to-be-detected sterile rice seeds every 7-15 days in multiple periods; when the growth process of partial plants which are sowed in the first period of the sterile rice seeds reaches a pollen mother cell meiosis period, namely a pulvinus peacetime, culturing sterile rice plants which are sowed in 3-4 continuous periods for a preset number of days in a certain processing environment and then taking the sterile rice plants out; recording the taking-out time of each sterile rice plant from the processing time, recording the heading data of a single ear of the sample, sampling, sleeving the single ear by a plastic bag, performing microscope examination, and testing a self-fertilized seed setting rate in maturation; and determining two important indexes of the sterility of the photo-thermo-sensitive genic male sterile rice, namely a critical sterility inducing temperature and a fertility alternation sensitive period according to the number of days between single-ear processing of the sample and heading, a pollen microscope examination result and a sleeving self-fertilized seed setting result by means of an index of number of days between each period in ear development and heading. The method has advantages of easy operation, and more accurate and more complete detecting result.

Description

technical field [0001] The invention belongs to the technical field of safe application detection of new materials in the utilization of rice heterosis, and in particular relates to two important indicators for detecting the sterility of photothermosensitive genic sterile rice, that is, the starting point temperature of sterility and the sensitive period of fertility conversion. Background technique [0002] The utilization of photothermo-sensitive genic male sterile rice (S) with stable sterility is the prerequisite for the popularization of two-line hybrid rice. Photothermosensitive genic male sterile rice is sensitive to light and temperature conditions. Generally, it is sterile under high temperature in long days and fertile in low temperature in short days. In the weather at the starting point temperature of sterility, the sterile line will self-fertilize, which will cause the failure of hybrid seed production and cause huge losses to production. Therefore, only by acc...

Claims

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Application Information

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IPC IPC(8): A01G1/00A01H1/00G01N33/00
CPCA01G22/00A01H1/00G01N33/00
Inventor 杜士云王辉王德正吴爽郑卫东
Owner RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
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