Method for detecting sterility of photo-thermo-sensitive genic male sterile rice according to multiphase and multi-sample method

Abstract

The invention discloses a method for detecting sterility of photo-thermo-sensitive genic male sterile rice according to a multiphase and multi-sample method. The method comprises the steps of sowing to-be-detected sterile rice seeds every 7-15 days in multiple periods; when the growth process of partial plants which are sowed in the first period of the sterile rice seeds reaches a pollen mother cell meiosis period, namely a pulvinus peacetime, culturing sterile rice plants which are sowed in 3-4 continuous periods for a preset number of days in a certain processing environment and then taking the sterile rice plants out; recording the taking-out time of each sterile rice plant from the processing time, recording the heading data of a single ear of the sample, sampling, sleeving the single ear by a plastic bag, performing microscope examination, and testing a self-fertilized seed setting rate in maturation; and determining two important indexes of the sterility of the photo-thermo-sensitive genic male sterile rice, namely a critical sterility inducing temperature and a fertility alternation sensitive period according to the number of days between single-ear processing of the sample and heading, a pollen microscope examination result and a sleeving self-fertilized seed setting result by means of an index of number of days between each period in ear development and heading. The method has advantages of easy operation, and more accurate and more complete detecting result.

Description

technical field [0001] The invention belongs to the technical field of safe application detection of new materials in the utilization of rice heterosis, and in particular relates to two important indicators for detecting the sterility of photothermosensitive genic sterile rice, that is, the starting point temperature of sterility and the sensitive period of fertility conversion. Background technique [0002] The utilization of photothermo-sensitive genic male sterile rice (S) with stable sterility is the prerequisite for the popularization of two-line hybrid rice. Photothermosensitive genic male sterile rice is sensitive to light and temperature conditions. Generally, it is sterile under high temperature in long days and fertile in low temperature in short days. In the weather at the starting point temperature of sterility, the sterile line will self-fertilize, which will cause the failure of hybrid seed production and cause huge losses to production. Therefore, only by acc...

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Problems solved by technology

For example, in the technical specifications for the two-line hybrid rice seed production system promulgated according to the national standard of the People's Republic of China (GB / T 29371.1-2012), during the treatment of sterile line materials, indica rice is the main stem and young panicle differentiation enters the end of stage IV, and japonica rice is the main When the differentiation of stems and young panicles enters the end of the third stage, they are transferred to an artificial climate box for treatment. However, this treatment method can only rely on continuous stripping tiller visual inspection to judge the progress of panicle development, which is cumbersome; in addition, in the part of the treatment results, the fertility observation objects At the end of the artificial climate chamber, mark the ears of flag leaf and inverted second leaf with a leaf pillow distance of 0cm-2cm, and take samples for microscopic inspection after marking the beginning of ear. This method only checks the ear temperature of ear development Ⅴ-Ⅵ. sensitivity, ignoring other possible fertility-sensitive periods

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