Ganoderma lucidum polysaccharide extracting method

A technology of Ganoderma lucidum polysaccharide and extraction method, which is applied in the field of biological extraction, can solve the problems of backward extraction process, poor anticancer activity of Ganoderma lucidum polysaccharide, and affect the color of Ganoderma lucidum polysaccharide, so as to achieve high anti-tumor activity, ensure the purity of the finished product, and ensure the effect of color

Active Publication Date: 2015-11-04
广州盛妍精细化工有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the backward extraction process of Ganoderma lucidum polysaccharides, the obtained product is yellowish brown. This is because the content of pigment in Ganoderma lucidum polysaccharides is relatively large, which affects the color of Ganoderma lucidum polysaccharides. Not only that, but the anticancer activity of Ganoderma lucidum polysaccharides with too high impurity content is also relatively low. Difference

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] 1) Break the Ganoderma lucidum, use 6 times the weight of 80v% ethanol to reflux and extract twice, reflux for 2 hours each time, filter, and take the filter residue for later use;

[0017] 2) The filter residue was reflux extracted twice with 6 times the weight of water, each time for 2 hours, filtered, and the filtrate was collected for subsequent use;

[0018] 3) Concentrate the filtrate to an extract with a relative density of 1.1 at 60°C, add 90v% ethanol to the extract until the ethanol content is 80v% to precipitate, and centrifuge to obtain the precipitate;

[0019] 4) Wash the precipitate with 2 times the weight of absolute ethanol and acetone respectively, dissolve the washed precipitate with 2 times of distilled water, pass through a hollow fiber membrane with a molecular weight cut-off of 50,000, collect the concentrated solution, and pass the concentrated solution through the cut-off A hollow fiber membrane with a molecular weight of 80,000, collect the per...

Embodiment 2

[0027] 1) Break the Ganoderma lucidum, use 12 times the weight of 95v% ethanol to reflux and extract 3 times, reflux for 3 hours each time, filter, and take the filter residue for later use;

[0028] 2) The filter residue was refluxed and extracted 3 times with 12 times the weight of water, each time for 3 hours, filtered, and the filtrate was collected for subsequent use;

[0029] 3) Concentrate the filtrate to an extract with a relative density of 1.3 at 60°C, add 95v% ethanol to the extract until the ethanol content is 85v% to precipitate, and centrifuge to obtain the precipitate;

[0030] 4) Wash the precipitate with 4 times the weight of absolute ethanol and acetone respectively, dissolve the washed precipitate with 4 times of distilled water, pass through a hollow fiber membrane with a molecular weight cut-off of 50,000, collect the concentrated solution, and pass the concentrated solution through the cut-off A hollow fiber membrane with a molecular weight of 80,000, col...

Embodiment 3

[0033] 1) Break the Ganoderma lucidum, use 10 times the weight of 90v% ethanol to reflux and extract twice, each time for 2 hours, filter, and take the filter residue for later use;

[0034] 2) The filter residue was reflux extracted twice with 10 times the weight of water, extracted for 2 hours each time, filtered, and the filtrate was collected for subsequent use;

[0035] 3) Concentrate the filtrate to an extract with a relative density of 1.2 at 60°C, add 95v% ethanol to the extract until the ethanol content is 85v% to precipitate, and centrifuge to obtain the precipitate;

[0036] 4) Wash the precipitate with 3 times the weight of absolute ethanol and acetone respectively, dissolve the washed precipitate with 3 times of distilled water, pass through a hollow fiber membrane with a molecular weight cut-off of 50,000, collect the concentrated solution, and pass the concentrated solution through the cut-off A hollow fiber membrane with a molecular weight of 80,000, collect th...

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Abstract

The invention discloses a ganoderma lucidum polysaccharide extracting method. The method comprises the following steps: (1), ganoderma lucidum ethanol is extracted, and filter residues are taken for standby application; (2), the filter residues are extracted with water, and a filtrate is collected for standby application; (3), the filtrate is concentrated into extract which is then precipitated with ethanol, and a precipitate is obtained through centrifugation; (4), the precipitate is sequentially washed with absolute ethyl alcohol and acetone respectively, the washed precipitate is dissolved with distilled water and passes through a hollow fiber membrane with the molecular weight cut-off being 50,000, a concentrated solution is collected and passes through a hollow fiber membrane with the molecular weight cut-off being 80,000, a permeate liquid is collected, centrifuged and filtered, and a filtrate is collected for standby application; (5), activated carbon is added to the filtrate for decoloration, the mixture is filtered, a filtrate is taken and subjected to spray drying, and ganoderma lucidum polysaccharide is obtained. The ganoderma lucidum polysaccharide has high purity, pure white color and high anti-tumor activity.

Description

technical field [0001] The invention belongs to the technical field of biological extraction, and in particular relates to an extraction method of ganoderma lucidum polysaccharide. Background technique [0002] Ganoderma lucidum polysaccharide is one of the important chemical components of Ganoderma lucidum, which has anti-tumor, immune regulation, hypoglycemic, hypolipidemic, anti-oxidative and anti-aging effects, and can also be used as an effective adjuvant drug for anti-tumor and radiotherapy. However, due to the backward extraction process of Ganoderma lucidum polysaccharides, the obtained product is yellowish brown. This is because the content of pigment in Ganoderma lucidum polysaccharides is more, which affects the color of Ganoderma lucidum polysaccharides. Not only that, but the anticancer activity of Ganoderma lucidum polysaccharides with too high impurity content is also relatively low. Difference. Contents of the invention [0003] The technical problem to be...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61P35/00
Inventor 文继承
Owner 广州盛妍精细化工有限公司
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