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Ganoderma lucidum spore powder inoxidizability visual analysis method

A technology of Ganoderma lucidum spore powder and anti-oxidation, which is applied in the direction of material analysis by observing the influence of chemical indicators, analysis by making materials undergo chemical reactions, color/spectral characteristic measurement, etc., to achieve easy availability of raw materials and low price , the effect of mild reaction conditions

Inactive Publication Date: 2015-11-04
HUBEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the research on the antioxidant activity of Ganoderma lucidum spore powder involves in vitro antioxidant activity, especially the visual analysis of the antioxidant activity of Ganoderma lucidum spore powder.

Method used

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  • Ganoderma lucidum spore powder inoxidizability visual analysis method
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  • Ganoderma lucidum spore powder inoxidizability visual analysis method

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Experimental program
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Effect test

Embodiment 1

[0028] (1) Add 2.00mL of 0.01mol / L salicylic acid solution and different amounts of 0.018mol / L FeSO to the 10mL colorimetric tube 4 solution, 0.50 mL of mass concentration of 30% H 2 o 2 Solution, dilute to 10mL with water, fully oscillate and mix, measure the absorbance value of the mixed solution after the reaction at 530nm, and obtain the following: figure 1 The absorbance value of the mixed solution after the reaction is shown for FeSO 4 Dosage curve, by figure 1 It can be seen that with the increase of the amount of FeSO4 solution, the absorbance of the mixed solution after the reaction increases. When the amount reaches 0.50mL, the absorbance value of the mixed solution after the reaction does not change much. 4 The optimal dosage of the solution is 0.50mL;

[0029] (2) Add 2.00mL 0.01mol / L salicylic acid solution, 0.50mL 0.018mol / L FeSO4 solution, different amounts of H2O2 (30%) solution in 10mL colorimetric tube, dilute to 10mL with water, fully Oscillate and mix,...

Embodiment 2

[0033] This embodiment is the same as Embodiment 1, the difference lies in step (4):

[0034] (4) Use the broken Ganoderma lucidum spore powder as a scavenger for hydroxyl radicals, add 0.02, 0.04, 0.10 g of broken Ganoderma lucidum spore powder particles to the colorimetric tube, and add 2.00 mL of 0.01mol / L salicylic acid solution , 0.50 mL FeSO 4 solution, 0.50 mL of H 2 o 2 solution, start the reaction, fully shake and mix, and the color of the mixed solution after the reaction is as follows Image 6 As shown, compare it with Figure 4 After the comparison, the ascorbic acid dosage parameter is obtained, and the ascorbic acid dosage parameter is brought into image 3 The standard curve of the shown ascorbic acid dosage to the scavenging rate of hydroxyl radicals finally obtains the result: the scavenging rate of 0.02, 0.04, 0.10g ganoderma lucidum spore powder particles is respectively 63%, 76% to the scavenging rate of hydroxyl radicals , 85%.

Embodiment 3

[0036] This embodiment is the same as Embodiment 1, the difference lies in step (4):

[0037] (4) Accurately weigh 3.0 g of Ganoderma lucidum broken spore powder, add 45.0 g of distilled water at a material-to-liquid ratio of 1:15, conduct ultrasonic extraction at 37° C. for 35 min, centrifuge the extract after the ultrasonic is finished, and concentrate the supernatant. Concentrate to 10 mL. Use the broken Ganoderma lucidum spore powder as a scavenger for hydroxyl radicals, add 0.3, 0.5, 0.9mL of broken Ganoderma lucidum spore powder particles to the colorimetric tube, add 2.00mL of 0.01mol / L salicylic acid solution, 0.50mL FeSO 4 solution, 0.50 mL of H 2 o 2 solution, start the reaction, fully shake and mix, and the color of the mixed solution after the reaction is as follows Figure 7 As shown, compare it with Figure 4 After the comparison, the ascorbic acid dosage parameter is obtained, and the ascorbic acid dosage parameter is brought into image 3 The standard cur...

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Abstract

The invention discloses a ganoderma lucidum spore powder inoxidizability visual analysis method. Salicylic acid is used as a developer of hydroxyl radical produced by a Fenton reaction and the produced dihydroxy-benzoic acid has the maximal absorptive amount at the wavelength of 530nm. According to the above principle, the optimal Fe<2+> and H2O2 use amounts of the reaction system are respectively determined, ascorbic acid and sporoderm-broken ganoderma lucidum spore powder are used as hydroxyl radical removal agents and the hydroxyl radical removal rate of the sporoderm-broken ganoderma lucidum spore powder is obtained. Through the standard contrast figure of reacted mixed solution colors corresponding to ascorbic acid solutions with different concentrations and a standard curve of the hydroxyl radical removal rates corresponding to the ascorbic acid use amounts, the reacted mixed solution colors can be compared so that ganoderma lucidum spore powder inoxidizability visual analysis is realized. The method has the advantages of simple processes, easily available raw materials, cheap price, mild reaction conditions and no toxicity and provides theoretical reference for ganoderma lucidum spore powder inoxidizability research and ganoderma lucidum spore powder application.

Description

technical field [0001] The invention belongs to the technical field of antioxidation analysis of ganoderma lucidum spore powder, and in particular relates to a visual analysis method for antioxidation of ganoderma spore powder. Background technique [0002] Ganoderma lucidum belongs to the Basidiomycetes Polyporaceae family and belongs to fungi. As a precious traditional Chinese medicine, it has high medicinal value. Ganoderma lucidum spores are extremely fine particles ejected from the cap during the growth and maturity of Ganoderma lucidum. It is the germ cell of Ganoderma lucidum, has all the genetically active substances of Ganoderma lucidum, and is rich in proteins, amino acids, glycopeptides, and triterpenoids. Scientific studies have shown that Ganoderma lucidum spore powder has various pharmacological effects such as liver protection, anti-tumor, blood sugar and blood lipid lowering, immune regulation, and anti-oxidation. Due to its rich chemical composition and va...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N21/78
Inventor 宋功武何瑜钟亚平邓淳刘潇
Owner HUBEI UNIV
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