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Method for detecting acrylamide concentration in solution

A technology of acrylamide and solution, which is applied in the field of chemical detection to achieve the effects of simple and convenient operation, improved sensitivity and reduced detection cost

Active Publication Date: 2015-11-04
深圳市威标检测技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the characteristic single-stranded DNA can be used as a sensing platform to detect acrylamide by electrochemical technology. So far, there are no reports on the use of characteristic single-stranded DNA modified gold electrodes for the detection of acrylamide.

Method used

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  • Method for detecting acrylamide concentration in solution
  • Method for detecting acrylamide concentration in solution

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Embodiment 1

[0040] An embodiment of the present invention provides a method for detecting the concentration of acrylamide in a solution, comprising:

[0041] A three-electrode system sensor is used to detect acrylamide in the sample solution by differential pulse voltammetry, and the concentration of acrylamide in the sample solution is obtained according to the differential pulse voltammetry curve of acrylamide, wherein, in the three-electrode system The working electrode is a gold electrode modified with a characteristic single-stranded DNA, and the sequence of the characteristic single-stranded DNA is 5'-TTTTT TTTTTGGGGG-(CH 2 ) 6 -SH-3'.

[0042] The present invention uses differential pulse voltammetry to detect acrylamide,

[0043] Differential pulse voltammetry superimposes a continuous pulse with constant amplitude and fixed pulse width on the linear scanning waveform. During the scanning process, the base potential is scanned from the initial potential to the final potential, a...

Embodiment 2

[0061] Electrochemical workstation: CHI760E;

[0062] Three-electrode system: the working electrode is a gold electrode modified with characteristic single-stranded DNA, the auxiliary electrode is a platinum electrode, and the reference electrode is an Ag / AgCl electrode;

[0063] Buffer solution: the first phosphate buffer solution with a pH value of 8.0 and a concentration of 0.2mol / L;

[0064] Standard stock solution: 2μmol / L standard acrylamide solution;

[0065] The gold electrodes have been treated before modification: the gold electrodes are placed on the polishing cloth containing the polishing powder with particle size of 1 μm, 0.3 μm, and 0.05 μm in turn to polish to the mirror surface, and then the gold electrodes are placed in acetone, concentration Sonicate 0.5 mol / L sulfuric acid solution and ultrapure water for 1 minute, and rinse with ultrapure water for 0.5 minutes after each ultrasound.

[0066] Modification of gold electrode: drop 7 μL containing 5×10 -6 m...

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Abstract

The present invention discloses a differential pulse voltammetry method for detecting acrylamide in a solution to be detected by using a three-electrode system. linear equation of acrylamide is employed to obtain the concentration of acrylamide in the solution to be detected, wherein the working electrode in the three-electrode system is a characteristic single-stranded DNA modified gold electrode, and the single-stranded DNA sequence is 5'-TTTTTTTTTTGGGGG- (CH2) 6-SH-3 '. The present invention utilizes differential pulse voltammetry method to carry out high sensitivity detection of acrylamide, achieves detection limit for acrylamide, and has the advantages of simpleness, rapidness, high sensitivity and selectivity, and very broad application prospects.

Description

technical field [0001] The invention belongs to the technical field of chemical detection, and in particular relates to a characteristic single-stranded DNA electrochemical sensor for detecting acrylamide concentration by differential pulse voltammetry. Background technique [0002] Acrylamide has mutagenic effects in both in vivo and in vitro tests, and can cause gene mutations and chromosomal abnormalities in mammalian somatic and germ cells, such as micronucleus formation, sister chromatid exchange, polyploidy, aneuploidy, and others Abnormal mitosis, etc., dominant lethal test was positive. It was also proved that the metabolite glycidamide of acrylamide is its main mutagenic active substance. Animal experiments have found that acrylamide can cause tumors in various organs of rats, including mammary gland, thyroid gland, testis, adrenal gland, central nervous system, oral cavity, uterus, and pituitary gland. The International Agency for Research on Cancer (IARC) evalua...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/48G01N27/30
Inventor 肖琦黄珊卢双燕盛家荣黄初升
Owner 深圳市威标检测技术有限公司
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