Bo-chrysanthemum total flavone extract preparation method based on response surface optimization

A response surface optimization and extraction technology, which is applied in the direction of medical preparations containing active ingredients, pharmaceutical formulas, plant raw materials, etc., can solve the problems of large investment, low extraction rate of water decoction, cumbersome operation and popularization of applications, etc. simple effect

Inactive Publication Date: 2015-11-11
ANHUI RUISIWEIER TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The extraction rate of the water decoction method is low, and new extraction processes such as ultrasonic extraction and supercritical extraction have attracted widespread attention due to their high extraction efficiency, but the above methods are limited due to problems such as excessive investment and cumbersome operations in production Popular application in industrial production

Method used

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  • Bo-chrysanthemum total flavone extract preparation method based on response surface optimization
  • Bo-chrysanthemum total flavone extract preparation method based on response surface optimization
  • Bo-chrysanthemum total flavone extract preparation method based on response surface optimization

Examples

Experimental program
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preparation example Construction

[0033] (3) Preparation of reference substance solution

[0034] Accurately weigh 10 mg of anhydrous rutin dried to constant weight in a 50 mL volumetric flask, add an appropriate amount of 95% ethanol to dissolve and constant volume, and shake well to obtain 0.200 mg / mL rutin control mother solution.

[0035] (4) Establishment of standard curve

[0036] Precisely measure 2.0mL, 4.0mL, 6.0mL, 8.0mL, 10.0mL, 12.0mL, 14.0mL, 16.0mL of rutin control mother solution into 25mL volumetric flask, add 1mL of 5% sodium nitrite solution, mix well, Stand for 6min, add 1mL of 10% aluminum nitrate solution, shake well, let stand for 6min, add 10mL of 10% sodium hydroxide solution, dilute to the mark with 30% ethanol, shake well, and let stand. After 15 minutes, measure the absorbance value at 510 nm. A blank solution without rutin was used as a reference. With the absorbance as the ordinate and the concentration of the rutin reference substance as the abscissa, draw a standard curve, and...

Embodiment 1

[0040] In this example, according to the method provided by the present invention, different conditions were selected to optimize the extraction of total flavonoids in Boju.

[0041] 1. Effect of ethanol concentration on total flavonoids content in Boju total flavonoids extract

[0042] Weigh 10g of chrysanthemum coarse powder, use 100mL concentration of 50%, 60%, 70%, 80%, 90% ethanol solution as extraction solvent, reflux extraction at 70°C for 2h, the obtained extract is cooled at 15 Centrifuge at a speed of 8000r / min for 10min at ℃ to obtain the corresponding extract of total flavonoids from Boju chrysanthemum. The absorbance and total flavonoid content of the obtained extract were measured according to the above method, as shown in Table 1. It can be seen that when the ethanol concentration is 80%, the content of total flavonoids in the extract is the largest.

[0043] The absorbance and total flavonoid content of Boju total flavonoids extract obtained under different e...

Embodiment 2

[0070] Example 2 Response Surface Extraction Design

[0071] Combined with the data obtained from the single factor condition optimization, the response surface extraction optimization was performed on the three factors of ethanol concentration, extraction time, and extraction times. Set the ethanol concentration as 70%, 80% and 90%, the extraction time as 2.5h, 3h and 3.5h, and the extraction times as 1 time, 2 times and 3 times, and 17 sets of data were obtained.

[0072] (1) Analysis of variance

[0073] figure 1 It is a schematic diagram of the variance analysis of the data, and the probability corresponding to the model is (0.0020)0.05, the irrational index is not significant, and the quadratic polynomial regression model is correct. The available data are suitable for response surface optimization design.

[0074] (2) Residual analysis

[0075] figure 2 It is a normal probability distribution graph of residuals, and the closer the points in the graph are to the str...

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Abstract

The invention discloses a Bo-chrysanthemum total flavone extract preparation method based on response surface optimization. The preparation method is characterized by comprises the following steps: grinding Bo-chrysanthemum, adding ethanol, carrying out reflux extraction, cooling the extract, subjecting the extract to centrifugation, and collecting the supernate namely the Bo-chrysanthemum total flavone extract; wherein the optimal conditions that influence the reflux extraction effect are determined through response surface experiments. The provided extraction method has the advantages that the operation is convenient and fast, the extraction cost is low, and no toxic or harmful substance is introduced into the final product (Bo-chrysanthemum wine). The optimal extraction factors are determined by the response surface design and optimization, and thus the optimal extraction factors are scientific.

Description

technical field [0001] The invention relates to a method for extracting total flavonoids in Boju, in particular to a method for extracting total flavonoids in Boju based on response surface optimization. Background technique [0002] Health-preserving and health-care wine has a history of thousands of years and is an important part of Chinese medicine science. With the advancement of science and technology, it has developed from the traditional technology of soaking Chinese medicine in wine to the functional health care wine made by extracting the active ingredients in Chinese medicine by using modern methods such as extraction, leaching and bioengineering. [0003] As a typical authentic medicinal material in Bozhou and one of the four famous chrysanthemums, the active ingredients of Boju are mainly flavonoids, which have the functions of dispelling wind and heat, relieving heat and improving eyesight, expanding coronary arteries, lowering blood pressure, preventing hyperli...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/287
Inventor 李安军陆玮汤有宏余秀娟梁绍勋刘国英周庆伍
Owner ANHUI RUISIWEIER TECH
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