Preparation method of gold nucleus-quantum dot satellite structure assembly

Abstract

The invention discloses a preparation method of a gold nucleus-quantum dot satellite structure assembly, belonging to the technical field of analytical chemistry. A high-yield assembly method of a gold nucleus-quantum dot satellite structure comprises the following steps of synthesis of gold nano particles with the particle size of 35nm, preparation of quantum dots with the particle size of 7nm, DNA-1 modification on the gold nano particles, complementary sequence DNA-2 modification on the quantum dots, assembling of the gold nucleus-quantum dot satellite structure, and purification of the gold nucleus-quantum dot satellite structure. By adopting the preparation method, the problems that quantum dot assembling is difficult and the yield is low are solved, and a way is paved for developing a fluorescence sensor based on quantum dot assembling.

Description

technical field

[0001] The invention relates to a high-yield assembly method of a gold core-quantum dot satellite structure, belonging to the technical field of analytical chemistry. Background technique

[0002] Quantum dots (QuantumDots, QDs) are nanoparticles composed of II-VI or III-V elements. The particle size of quantum dots is generally between 1 and 10 nm. Since electrons and holes are quantum-confined, the continuous energy band structure becomes a discrete energy level structure with molecular characteristics, and can emit fluorescence after being excited. Compared with traditional fluorescent dyes, QDs have broad excitation, narrow emission and high fluorescence quantum yield. QDs have gradually replaced dyes as a new type of biomarker material, and are widely used in cell imaging, immunofluorescence, in vivo imaging, and nucleic acid recognition.

[0003] Gold nanoparticles have good biocompatibility and large specific surface area. Utilizing its quenching ef...

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