Pretreatment kit for rapid detection of 28 polychlorinated biphenyls in aquatic products and its application
A technology of polychlorinated biphenyls and kits, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of low throughput, high cost, slow speed, etc., and achieve good reproducibility, simple operation method, and selectivity Good results
Active Publication Date: 2017-04-12
FUQING ENTRY EXIT INSPECTION & QUARANTINE BUREAU OF P R C
View PDF2 Cites 0 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
[0007] The purpose of the present invention is to develop an easy-to-operate, high-sensitivity, accurate-result technology for the slow, low-efficiency, high-cost, low-throughput and other deficiencies in the existing pre-treatment technologies for the detection of 28 kinds of PCBs in aquatic products. A pretreatment kit capable of simultaneously quantitatively detecting the content of 28 polychlorinated biphenyls and its application
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0034] Take Pomfret as a sample:
[0035] Add 2.00 g of pomfret sample to a 50 mL plugged centrifuge tube containing reagent I, add 35.0 mL of dichloromethane + n-hexane (v / v=1 / 1) mixture, and disperse and homogenize at a high speed of 11000 rpm for extraction Centrifuge the tube for 1 min at 4500 r / min for 5 min, and take the supernatant; pour all the supernatant into a 50 mL plugged centrifuge tube filled with reagent II, vortex for 1 min, and centrifuge the tube at 4500 r / min Centrifuge at a rotating speed for 5 min; all the supernatant was poured into a 100 mL heart-shaped bottle, evaporated to near dryness at 40°C, fixed to volume with 2.00 mL of n-hexane and filtered through a 0.22 μm filter membrane for analysis.
[0036] Formulation of matrix matching working curve: Take appropriate amount of 28 kinds of PCB mixed standard substances, prepare 1000 μg / L standard stock solution with n-hexane, and store in -4 ℃ refrigerator for later use;
[0037] Weigh 2.00 g of blank p...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
| Property | Measurement | Unit |
|---|---|---|
| The average particle size | aaaaa | aaaaa |
| Particle size | aaaaa | aaaaa |
Login to View More
Abstract
The invention relates to a rapid-detection pretreatment kit for 28 polychlorinated biphenyls in an aquatic product and application of the kit. The kit is composed of a reagent I and a reagent II, wherein the reagent I is prepared by mixing the following raw materials: 1.0 g of sodium chloride, 2.0 g of anhydrous sodium sulfate, and 2.0 g of magnesium aluminum hydroxide carbonate; the reagent II is prepared by mixing the following raw materials: 4.0 g of acidized silica gel and 2.0 g of anhydrous sodium sulfate. By adopting the prepared kit and the application method thereof provided by the invention for detecting 28 polychlorinated biphenyls in the aquatic product, expensive pretreatment equipment for removing fat in a sample is not required to be equipped, a complex, low-efficiency and low-flux column chromatography pretreatment process is not used and the kit has the advantages of high detecting speed, high sensitivity, high selectivity and high accuracy and can meet needs for detecting 28 polychlorinated biphenyls in the aquatic product sample of a conventional laboratory. By adopting the kit provided by the invention, the contents of 28 polychlorinated biphenyls in the aquatic product sample can be determined simultaneously and the kit has the characteristics of being simple, convenient, rapid and accurate.
Description
technical field [0001] The invention belongs to the technical field of food safety detection, and relates to a pretreatment kit for rapid detection of 28 polychlorinated biphenyls in aquatic products and an application thereof. Background technique [0002] Polychlorinated biphenyls (PCBs) are a class of artificially synthesized organic compounds that are difficult to decompose under natural conditions and have high residues. They have been listed as one of the environmental persistent pollutants. This kind of substance can be enriched in the fat of animals, and has "teratogenic, mutagenic, and carcinogenic" hazards to organisms, and its chemical properties are stable and difficult to be degraded. At the same time, it can migrate through various carriers and transfer to humans through the food chain. In vivo enrichment. The Global Environmental Monitoring System / Food Planning Section (GEMS / Food) stipulates 7 polychlorinated biphenyl homologue isomers as indicator PCBs (Indi...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More IPC IPC(8): G01N30/06
Inventor 丁立平蔡春平郑香平张睿郭菁许仁杰
Owner FUQING ENTRY EXIT INSPECTION & QUARANTINE BUREAU OF P R C