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Method for breeding pinus massoniana good group asexual tissue culture seedlings

A technology of masson pine and tissue culture seedlings, which is applied in the field of plant propagation, can solve the problems of restricting the rapid breeding of masson pine tissue culture seedlings, the low germination and transformation rate of somatic embryo seedlings, and the low proliferation coefficient of secondary buds, etc., and achieve the goal of seedling cultivation The effect of shortening the cycle, solving the shortage of good seeds, and being significantly innovative and progressive

Active Publication Date: 2015-11-18
GUANGXI FORESTRY RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Many domestic scholars have done a lot of research on somatic embryogenesis and organogenesis technology of Pine massoniana, among which somatic embryogenesis technology of Pine massoniana mainly focuses on somatic embryo induction, proliferation and mature culture, and the obvious effect on somatic embryo germination and transformation It is still rare, and the main reason is that the germination and transformation rate of somatic embryo seedlings are not high, the root quality is poor, the seedling yield is low, and the technical applicability is not strong
However, the tissue culture and rapid propagation technology of Pine massoniana through the organogenesis pathway has seriously restricted the rapid propagation of Pine massoniana tissue culture seedlings due to the low proliferation coefficient of subculture buds and long cultivation period.

Method used

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  • Method for breeding pinus massoniana good group asexual tissue culture seedlings

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Embodiment 1

[0029] The invention discloses a method for breeding asexualized tissue-cultured seedlings of excellent populations of Pinus massoniana. The immature cotyledon prophase zygotic embryos of an elite family of Pinus massoniana are used as explants, and a large number of vigorous embryogenic cell lines of Pinus massoniana are obtained through somatic embryogenesis. Wherein, the embryogenic cell line SE-3 produced by mature culture is opaque white, has a waxy luster, and mature somatic embryos developed into the cotyledon stage are placed in a germination medium for germination; the germination medium is: 1 / 2 modified MS medium + activated carbon 10g L -1 + Glucose 20g·L -1 + Lactose 5g·L -1 + sucrose 5g·L -1 . The cultivation control conditions are: dark cultivation, no light, cultivation temperature 20±1°C. After one week of cultivation, the germination rate reached 97.4%.

[0030]After the leaves of the germinated somatic embryos turn green, the base of the hypocotyl turns ...

Embodiment 2

[0036] The invention discloses a method for breeding asexualized tissue-cultured seedlings of excellent populations of Pinus massoniana. The immature cotyledon prophase zygotic embryos of an elite family of Pinus massoniana are used as explants, and a large number of vigorous embryogenic cell lines of Pinus massoniana are obtained through somatic embryogenesis. Wherein, the embryogenic cell line SE-3 produced by mature culture is opaque white, has a waxy luster, and mature somatic embryos developed into the cotyledon stage are placed in a germination medium for germination; the germination medium is: 1 / 2 modified MS medium + activated carbon 10g L -1 + Glucose 20g·L -1 + Lactose 5g·L -1 + sucrose 5g·L -1 . The cultivation control conditions are: dark cultivation, no light, cultivation temperature 20±1°C. After 2 weeks of cultivation, the germination rate reached 98.1%.

[0037] After the leaves of the germinated somatic embryos turn green, the base of the hypocotyl turns ...

Embodiment 3

[0043] The invention discloses a method for breeding asexualized tissue-cultured seedlings of excellent populations of Pinus massoniana. The immature cotyledon prophase zygotic embryos of an elite family of Pinus massoniana are used as explants, and a large number of vigorous embryogenic cell lines of Pinus massoniana are obtained through somatic embryogenesis. Wherein, the embryogenic cell line SE-3 produced by mature culture is opaque white, has a waxy luster, and mature somatic embryos developed into the cotyledon stage are placed in a germination medium for germination; the germination medium is: 1 / 2 modified MS medium + activated carbon 10g L -1 + Glucose 20g·L -1 + Lactose 5g·L -1 + sucrose 5g·L -1 . The cultivation control conditions are: dark cultivation, no light, cultivation temperature 20±1°C. After 3 weeks of cultivation, the germination rate reached 98.6%.

[0044] After the leaves of the germinated somatic embryos turn green, the base of the hypocotyl turns ...

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Abstract

The invention discloses a method for breeding pinus massoniana good group asexual tissue culture seedlings. The method comprises the following processes of somatic embryo germination, somatic embryo seedling elongation, multiplication culture, clumpy bud elongation and rooting culture, to be specific, mature pinus massoniana somatic embryos are placed in a germination culture medium and then transferred to an elongation culture medium for culture after germinating into bud seedlings, after bud seedlings elongate, root systems are cut off, the bud seedlings are transferred to multiplication culture medium for clumpy bud induction, induced clumpy buds are transferred to an elongation culture medium for elongation, and after bud seedlings are 2-3 cm in height, bud seedlings are cut off separately and then placed in a rooting culture medium for rooting treatment, so that pinus massoniana good group asexual tissue culture seedlings are obtained. By adopting the method for efficiently breeding pinus massoniana good group asexual tissue culture seedlings, lots of high-quality pinus massoniana excellent tissue culture seedlings can be rapidly and efficiently obtained, the problems that somatic embryo seedlings are relatively poor in root system and poor in growth in somatic embryogenesis, and bud seedlings are low in reproduction rate and long in breeding cycle in organogenesis are solved and obvious economic and social benefits are realized.

Description

technical field [0001] The invention belongs to the technical field of plant propagation, and relates to tissue culture seedling raising technology, in particular to a method for breeding asexual tissue culture seedlings of excellent populations of masson pine. Background technique [0002] Masson pine ( Pinus massonianan ) is native to my country and is one of the important tree species in barren hills greening, papermaking raw material forests, resin mining forests, and natural landscape forests in southern my country. It is widely distributed in my country. As a tree species with high comprehensive utilization value and great promotion potential, masson pine can not only be used to produce three-board, papermaking and chemical fiber industry manufacturing, but also can be used to produce industrial raw materials such as rosin and turpentine. In recent years, with the intensification of contradictions between people's growing living needs and the shortage of timber resour...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 姚瑞玲王胤
Owner GUANGXI FORESTRY RES INST
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