The method of microbial flocculant and activated carbon to fix flocculation and sedimentation oily yeast
A microbial flocculant and oil yeast technology, applied in the field of microorganisms, can solve the problems of high cost and complicated process, and achieve the effects of simple operation, efficient flocculation and sedimentation, and high flocculation sedimentation rate.
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[0025] (3) Preparation of the culture medium of Lipoyeast Stari
[0026] Inoculate Lipoyces studleyi with the bacterial strain number CGMCC NO: 2.1560 stored on the slant into sterile seed culture medium, and the dosage of each component in the described Lipoyces studleyi seed culture medium is respectively: glucose ; (NH 4 ) 2 SO 4 ; Yeast powder; KH 2 PO 4 ;MgSO 4 ·7H 2 O; pH=6.0; Cultivate on a shaker at 22°C at 150 rpm for 1 day to obtain a cultured L. stariae suspension, and store the obtained suspension in a refrigerator at 4°C for future use;
[0027] (4) The immobilization of activated carbon to the strain No. CGMCC NO: 2.1560 of Lipoyeast stariae
[0028] Take the suspension of L. starii obtained in step (3) and put it into a sterile nitrogen-limited medium. The dosage of each component in the nitrogen-limited medium is: glucose; (NH 4 ) 2 SO 4 ; Yeast powder; KH 2 PO 4 ;MgSO 4 ·7H 2 O; pH=6.5; take the activated carbon obtained in step (1), add it to th...
Embodiment 1
[0031] (1) Treatment of activated carbon
[0032] Take 20 g of 300-mesh activated carbon and dry it at 200°C for 2 h; after cooling to room temperature, weigh the activated carbon and water according to the volume ratio of activated carbon and water as 1:3; stir the activated carbon and water evenly, and add 38% sulfuric acid; fully stirred for 2 hours at a water bath temperature of 80°C; then suction filtered, and the activated carbon was washed several times with desalted water until the pH of the filtrate was neutral; the filtered activated carbon was dried at 200°C; The activated carbon was baked in a muffle furnace at 300°C for 2.5 h and then placed in a desiccator for later use;
[0033] (2) Preparation of microbial flocculant by Aspergillus sojae
[0034] Inoculate Aspergillus sojae (Aspergillus sojae) with the strain number CGMCC NO: 3.5231 stored on the slant into 100 mL of sterile fermentation medium. The dosage of each component in the Aspergillus sojae fermentatio...
Embodiment 2
[0045] Including the same steps (1), (2) and (3) as in Example 1, the difference steps are:
[0046] (4) The immobilization of activated carbon to the strain No. CGMCC NO: 2.1560 of Lipoyeast stariae
[0047] Take 10 mL of the suspension of L. starii obtained in step (3) and put it into 100 mL of sterile nitrogen-limited medium. The dosage of each component in the nitrogen-limited medium is: glucose 80.0 g / L; (NH 4 ) 2 SO 4 1.0 g / L; yeast powder 0.8 g / L; KH 2 PO 4 1.0 g / L; MgSO 4 ·7H 2 O 1.0 g / L; pH=6.5; take 4 g of activated carbon obtained in step (1), add it to the above 100 mL nitrogen-limited medium that has been inoculated with Lipoyeast stariae, and culture it on a shaker at 22°C at 150 rpm In 3 days, the suspension of Lipozyme spp. immobilized by activated carbon was obtained;
[0048] (5) Microbial flocculant flocculation and sedimentation activated carbon immobilized strain No. CGMCC NO: 2.1560 Lipoyeast stariae
[0049] Take 0.2 mL of the microbial flocc...
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