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Preparation process of botanical insecticide capable of preventing and treating aphids and acarids

A botanical pesticide and a preparation process technology, applied in the field of biological pesticides, can solve the problems of unsatisfactory activity, undetermined active chemical components, insufficient plant resources, etc. Resourceful Effects

Active Publication Date: 2015-11-25
LANZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because the plant resources are not abundant enough, or the activity is not very ideal, many studies have failed to achieve further results, and most of them have not determined the active chemical components

Method used

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  • Preparation process of botanical insecticide capable of preventing and treating aphids and acarids
  • Preparation process of botanical insecticide capable of preventing and treating aphids and acarids
  • Preparation process of botanical insecticide capable of preventing and treating aphids and acarids

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1 Preparation of different active parts of red clover

[0030] 1. Preparation of red clover extract: collect fresh aboveground parts of red clover plants, dry in the shade, and pulverize. Add 20 times the volume of ethanol, ultrasonically extract for 30 minutes, and repeat the extraction 3 times. Concentrate the extraction solvent under reduced pressure to obtain the extract, and dry it in a ventilated and dark place to obtain the red clover extract.

[0031] 2. Preparation of different active parts of red clover: take 20 g of the obtained red clover extract, dissolve it in 100 mL of distilled water with 5 times the volume, add 300 mL of petroleum ether 3 times the volume of the aqueous solution into the aqueous solution, vibrate vigorously, and let stand The layers were separated, and the supernatant was the petroleum ether layer; the extraction was repeated 3 times, the petroleum ether layers were combined, and concentrated under reduced pressure to obtain...

Embodiment 2

[0032] The insecticidal activity of different active parts of embodiment 2 to peach aphid

[0033] Refer to the reported authoritative classic test method - drop method (ZhaoQ.Q., LiY.Q., XiongL.X., etal.J.Agric.FoodChem.2010, 58, 4992-4998.).

[0034] Insecticides Weigh 1-2 mg of petroleum ether, ethyl acetate, and different active parts of the water phase prepared above, add acetone solution containing 10% distilled water to prepare a mother liquor with a concentration of 1.00 mg / mL, and then distill the mother liquor in turn with distilled water Dilute to 0.50, 0.25, 0.10 and 0.05 mg / mL solution, add surfactant JFC (C7-C9 alkanol polyoxyethylene ether) of 1% of the volume of the liquid medicine, and mix evenly to obtain the insecticide. Concentrations of the configuration liquid are respectively. Then use a micro-dropper to drip the medicinal solution on the back of the abdomen of peach aphids. After the dripping is completed, each treatment is put into a petri dish (30 in...

Embodiment 3

[0040] Example 3 The insecticidal activity of different active parts to cabbage aphids

[0041] The activity test method is the same as the activity method for green peach aphid. The test results of the activity of different parts of red clover against cabbage aphid are shown in Table 3. Among them, at a concentration of 1 mg / mL, after 24 hours of treatment, the contact killing activity of the ethyl acetate part on the cabbage aphid was above 60%; after 48 hours of treatment, the contact killing activity of the ethyl acetate part on the cabbage aphid reached more than 80%. The activity of both site and aqueous phase was greater than 60%. From Table 4, we know that the activity of red trifolium ethyl acetate is higher than that of petroleum ether and water phase. These results are basically consistent with the test results of peach aphid.

[0042] Table 3 Contact activity of different active parts of red clover against cabbage aphid

[0043]

[0044] Table 4 LC of differ...

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Abstract

The invention discloses a botanical insecticide capable of preventing and treating aphids and acarids. The main ingredient of the botanical insecticide capable of preventing and treating aphids and acarids is a trifolium pratense extract. A trifolium pratense botanical pesticide provided by the invention has obvious contact toxicity on the aphids and the acarids, is low in pesticide resistance generation possibility, is environment-friendly and is safe to non-target organisms such as people and livestock. Main functional ingredients are separated and identified as flavonoid compounds, so that a premise is provided for further development and utilization. The botanical insecticide is rich in plant resources and simple in preparation process and has favorable development and application prospects and economic values in agricultural production.

Description

technical field [0001] The invention relates to a preparation process of a botanical insecticide for preventing and controlling aphids and mites, and belongs to the technical field of biological pesticides. Background technique [0002] Aphids, also known as greasy insects and honey insects, are a class of herbivorous insects. There are about 4,400 species of aphids that have been discovered in 10 families, most of which belong to the family Aphididae. Aphids are one of the most destructive pests on earth. About 250 of them are serious pests for agriculture, forestry and horticulture. Aphids are widely distributed throughout the world, but mainly in temperate regions. The fecundity of aphids is very strong, and it can reproduce 10 to 30 generations a year, and the phenomenon of overlapping generations is prominent. Female aphids are fertile once born and do not require males to reproduce (ie parthenogenesis). [0003] Mites belong to the class Arachnida and the subclass...

Claims

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Application Information

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IPC IPC(8): A01N65/20A01N43/16A01P7/02A01P7/04
Inventor 刘权彭爱风李晓蓓
Owner LANZHOU UNIVERSITY
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