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Interventional effect of cyanidin-3-O-glucoside on reproductive toxicity of 1,3-dichloro-2-propanol

A technology of glucoside and cyanidin, applied in the field of medicine, can solve problems such as hazards, life-threatening, and weight loss of organisms, and achieve significant research results

Active Publication Date: 2015-11-25
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Research reports have shown that the main targets of 1,3-DCP toxicity are the reproductive system and kidneys. Long-term intake will lead to a series of harmful effects such as weight loss, male sterility and renal function decline, and even cause danger to life

Method used

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  • Interventional effect of cyanidin-3-O-glucoside on reproductive toxicity of 1,3-dichloro-2-propanol
  • Interventional effect of cyanidin-3-O-glucoside on reproductive toxicity of 1,3-dichloro-2-propanol
  • Interventional effect of cyanidin-3-O-glucoside on reproductive toxicity of 1,3-dichloro-2-propanol

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 MTT method to screen the concentration of 1,3-DCP on R2C cells and the concentration of C3G intervention

[0039] 1. MTT method to screen the concentration of 1,3-DCP on R2C cells

[0040] (1) Experimental method

[0041] 1) Cells were cultured to the logarithmic phase, digested with trypsin, centrifuged at 1500r / min for 5min, and diluted to 4×10 4 cells / mL, spread on a 96-well plate, and add about 200 μL of suspension to each well;

[0042] 2) After 24 hours, add different concentrations of 1,3-DCP to the corresponding groups, the concentration of each group is 1, 2, 4, 6, 8 mmol / L, 5 replicate wells in each group, and each group is repeated 3 times , and set a cell-free blank group;

[0043] 3) After 24 hours, absorb the liquid, add 10 μL of 5 mg / mL MTT reagent to each well, and place in a 37°C incubator;

[0044] 4) After 4 hours of MTT action, remove the liquid in the well and replace it with 200 μL of DMSO reagent, place the 96-well plate on a shake...

Embodiment 2

[0055] Embodiment 2 MTT method measures the inhibitory effect of C3G on 1,3-DCP in terms of cell activity

[0056] 1. Experimental method

[0057] (1) Cell culture and seeding Cell culture to the logarithmic phase, digest with trypsin, centrifuge at 1500r / min for 5min, and dilute the precipitate to 4×10 4 cells / mL, spread on a 96-well plate, and add about 200 μL of suspension to each well.

[0058] (2) 24 hours after inoculating cells in a 96-well plate, add C3G solution diluted with F12 culture medium (except the control group) to the corresponding groups in one of the 96-well plates, so that the final concentration of C3G in each well of each group is 5, 10, 20, 40 μmol / L. Add C3G solution and 1,3-DCP solution diluted in F12 culture medium to another 96-well plate, except for the control group, make the C3G in other groups be 5, 10, 20, 40 μmol / L respectively, 1,3-DCP in each well of each group The concentration of 3-DCP is 2mmol / L. The settings of the duplicate wells ...

Embodiment 3

[0063] Example 3 Intervention of C3G on morphological changes of R2C cells caused by 1,3-DCP detected by fluorescence microscopy

[0064] 1. Experimental method

[0065] (1) After resuspending the logarithmic phase cells and diluting the cells, inoculate 1 mL (approximately 20,000 cells) in each well of a 6-well plate.

[0066] (2) After 24 hours, add C3G solution and 1,3-DCP solution diluted with F12, except the first group, so that the C3G added in each well of each group is 5, 10, 20, 40 μmol / L, each group The 1,3-DCP concentration of the wells was 2 mmol / L.

[0067] (3) Stimulate each concentration of C3G and 1,3-DCP for 24 hours, check the cell morphology with a fluorescence microscope and take pictures.

[0068] 2. Observation of cell morphology by fluorescence microscope Figure 4 shown. The results showed that, compared with the cells in the control group, under the action of 2 mmol / L 1,3-DCP, the shape of R2C cells was significantly smaller and the number of cel...

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Abstract

The invention discloses application of cyanidin-3-O-glucoside in preparing medicine for preventing and / or treating male genital diseases caused by 1,3-dichloro-2-propanol. Research results show that the C3G has a significant inhibitory effect on R2C cell activity decline, cell morphological damage, increase in cell apoptosis rate and the like exposed by 1,3-DCP, the C3G, by promoting the expression of StAR, 3beta-HSD and like key protein, intervenes in R2C cell progesterone synthesis exposed by the 1,3-DCP and simultaneously the C3G, by reducing ROS (reactive oxygen species) inside the R2C cell exposed by the 1,3-DCP and further inhibiting MMP (mitochondrial membrane potential) and the like, intervenes in R2C cell apoptosis and progesterone expression function injury caused by the 1,3-DCP. The research achievement of the invention provides an important basis for human to prevent and treat male genital diseases caused by 1,3-dichloro-2-propanol and has a great research significance.

Description

technical field [0001] The invention belongs to the technical field of medicine. More specifically, it concerns the intervention of cyanidin-3-O-glucoside on the reproductive toxicity of 1,3-dichloro-2-propanol-exposed R2C cells. Background technique [0002] In recent years, chloropropanols, a contaminant in food, have attracted more and more attention worldwide. Since chloropropanols were found in soy sauce exported from Hong Kong in 1999, the investigation and research on this chemical pollutant more human attention. 3-chloropropanols are widely found in food, especially sauces and condiments such as soy sauce and oyster sauce. It is known that chloropropanols can be produced in various stages of food processing or during storage, and the two most important forms of chloropropanols in terms of toxicity levels are 1,3-dichloro-2-propanol (1, 3-DCP) and 3-chloro-1,2-propanediol (3-MCPD). The Joint FAO / WHO Expert Committee announced in 1993 that because of its carcinogen...

Claims

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Application Information

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IPC IPC(8): A61K31/7048A61P39/02
Inventor 白卫滨孙建霞胡云峰焦睿黄亚东
Owner JINAN UNIVERSITY
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