Lactobacillus bacterial strain capable of expressing and secreting human interferon Alpha2b and application thereof

Abstract

Symbiotic bacteria generally mainly producing Lactobacillus bacteria producing H2O2 are distributed on vaginal mucosa of a healthy woman of childbearing age, and the Lactobacillus bacteria functions in protectively preventing urinary and reproductive system infections. Multiple Lactobacillus bacteria are already provided with resistance to, for example, HIV virus by bioengineering so as to prevent the urinary and reproductive system infections. Lactobacillus jensenii producing H2O2, a principle member in the vaginal microbial flora, is reconstructed by bioengineering, so that the Lactobacillus jensenii is enabled to produce and secrete a powerful broad-spectrum antiviral molecule, human IFN Alpha-2b.

Description

technical field [0001] The invention relates to the field of molecular biology, and more specifically relates to an expression plasmid of human interferon alpha 2b and a genetically engineered bacterial strain expressing the protein. Background technique [0002] Most viruses, including human papillomavirus (HPV) and human herpes simplex virus (HSV), usually invade the host at mucosal surfaces. HPV infection has become a worldwide epidemic, with more than 6 million new cases each year in the United States alone. Every year millions of women worldwide are found to have potentially precancerous, abnormal Pap smears associated with HPV infection. For cervical lesions associated with HPV infection, there is no feasible drug treatment. Instead, efforts have been made to perform surgical or other excisional techniques to destroy the infected tissue and prevent further disease progression. These practices are usually restricted to more advanced patients due to undesired side eff...

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These treatments often require high drug doses, strict drug storage conditions and high production costs

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