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Entomopathogenic nematode symbiotic bacteria strain and application thereof

A technology of entomopathogenic nematodes and symbiotic bacteria, applied in the direction of application, nematicides, bacteria, etc., can solve food safety problems, environmental problems, problems affecting human health and foreign exchange earnings from agricultural product exports, and achieve increased added value and convenient production and use , The effect of large market development potential

Active Publication Date: 2016-07-27
NANJING AGRICULTURAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the widespread use of chemical pesticides has brought a series of environmental and food safety issues; it has seriously affected human health and the export of agricultural products

Method used

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  • Entomopathogenic nematode symbiotic bacteria strain and application thereof
  • Entomopathogenic nematode symbiotic bacteria strain and application thereof
  • Entomopathogenic nematode symbiotic bacteria strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The entomopathogenic nematode symbiotic bacterial strain R187-2 glycerol bacteria original species of the present invention is in NBTA culture medium (beef extract 0.5%, beef peptone 1%, agar 2%, red nitrogen tetrazole 0.0025%, bromofen blue 0.004%, pH7.2~7.4) on the plate for 24h, and its insecticidal activity was measured, and the red single colony with high activity was inoculated on the slant of test tube LB for later use. Take the test tube seed and inoculate it into a 1000mL shake flask with 180mL of LB medium, and cultivate it at a constant temperature of 30°C and 180 rpm with shaking until the logarithmic phase; prepare to inoculate the seed tank. Put 400L of culture medium into a 500L seed tank, the matrix formula is: glucose 0.6-0.8%, yeast extract 0.03-0.3%, sodium chloride 0.2-0.4%, FeCl30.001%, pH value 7.2-7.5. After feeding, sterilize at 121°C for 20-30 minutes, cool to 30°C, and inoculate 500L seed tanks with 8-10% of the cultured strains in the shaker f...

Embodiment 2

[0028] Example 2: Preparation of Entomopathogenic Nematode Symbiotic Bacteria Strain R187-2 Biological Insecticide

[0029]The entomopathogenic nematode symbiotic bacterial strain R187-2 glycerol bacteria original species of the present invention is in NBTA culture medium (beef extract 0.5%, beef peptone 1%, agar 2%, red nitrogen tetrazole 0.0025%, bromofen blue 0.004%, pH7.2~7.4) on the plate for 24h, and its insecticidal activity was measured, and the red single colony with high activity was inoculated on the slant of test tube LB for later use. Take the test tube seed and inoculate it into a 1000mL shake flask with 180mL of LB medium, and cultivate it at a constant temperature of 30°C and 180 rpm with shaking until the logarithmic phase; prepare to inoculate the seed tank. Put 400L of culture medium into a 500L seed tank, the matrix formula is: glucose 0.8%, yeast extract 0.3%, sodium chloride 0.4%, FeCl 3 0.001%, pH 7.2. After feeding, sterilize at 121° C. for 30 minutes...

Embodiment 3

[0030] Example 3: Preparation of Entomopathogenic Nematode Symbiotic Bacteria Strain R187-2 Biological Insecticide

[0031] The entomopathogenic nematode symbiotic bacterial strain R187-2 glycerol bacteria original species of the present invention is in NBTA culture medium (beef extract 0.5%, beef peptone 1%, agar 2%, red nitrogen tetrazole 0.0025%, bromofen blue 0.004%, pH7.2~7.4) on the plate for 24h, and its insecticidal activity was measured, and the red single colony with high activity was inoculated on the slant of test tube LB for later use. Take the test tube seed and inoculate it into a 1000mL shake flask with 180mL of LB medium, and cultivate it at a constant temperature of 30°C and 180 rpm with shaking until the logarithmic phase; prepare to inoculate the seed tank. Put 400L of culture medium into a 500L seed tank, the matrix formula is: glucose 0.7%, yeast extract 0.15%, sodium chloride 0.3%, FeCl 3 0.001%, pH 7.4. After feeding, sterilize at 121° C. for 30 minut...

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Abstract

The invention discloses an entomopathogenic nematode symbiotic bacteria strain which is characterized in that the class of the microorganism is named high-virulent strain R187-2 of serratia nematodiphila and collected in the China Center for Type Culture Collection on May 3rd, 2016, wherein the strain collection number is CCTCC M 2016240. The invention also discloses a biological insecticide as well as a preparation method and application thereof. The novel entomopathogenic nematode symbiotic bacteria insecticidal bacterial agent provided by the invention has the advantage of convenient production and use and is suitable for producing green pollution-free agricultural products in the modern ecological agriculture economy. The entomopathogenic nematode symbiotic bacteria strain is of great significance to protecting the ecological environment and improving the added value of agricultural products. Experiments prove that the liquid bacterial agent and solid bacterial agent of the R187-2 strain realize a good insecticidal effect, and the death rate of each group is 85% or more. With a wide insecticidal spectrum, the entomopathogenic nematode symbiotic bacteria strain R187-2 has a huge market development potential.

Description

technical field [0001] The invention relates to an entomopathogenic nematode symbiotic bacterial strain, belongs to the field of biotechnology, uses biological control methods to control the population of agricultural insects, and is suitable for the production of green and pollution-free agricultural products in modern ecological agricultural economy. Background technique [0002] According to incomplete statistics, the annual loss of crops worldwide due to pests and diseases accounts for about 37% of their total output. The prevention and control of pests and diseases has always been one of the ways for people to increase crop yields and develop agricultural production. From inorganic pesticides to organic synthetic pesticides, Chemical agents were once an effective means of controlling pests and diseases. However, the widespread use of chemical pesticides has brought a series of environmental and food safety issues; it has seriously affected human health and the export of...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P5/00C12R1/425
CPCA01N63/00C12N1/20C12N1/205C12R2001/425
Inventor 张克云徐漫柳李旺詹成修林建严秀文莫国香舒自美徐良
Owner NANJING AGRICULTURAL UNIVERSITY
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