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A method for detecting the effective components of Ganoderma lucidum spore powder raw material

A technology of Ganoderma lucidum spore powder and active ingredients, which is applied in the field of establishing and determining active ingredients in Ganoderma lucidum spore powder raw materials and its products, which can solve the problems of phosphate insoluble in organic solvents, shortened service life, and easy to separate out after improper use.

Active Publication Date: 2016-08-24
SHANGHAI ACAD OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] At present, mobile phases containing phosphate buffer and mobile phases without phosphate buffer are often used to separate nucleosides (Zhou Huiyan et al. 2011). Phosphate is difficult to dissolve in organic solvents, and it is easy to separate out when used improperly, which reduces the retention capacity of the chromatographic column. Decrease, reduce column efficiency and shorten its service life

Method used

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  • A method for detecting the effective components of Ganoderma lucidum spore powder raw material
  • A method for detecting the effective components of Ganoderma lucidum spore powder raw material
  • A method for detecting the effective components of Ganoderma lucidum spore powder raw material

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Effect test

Embodiment 1

[0135] The pretreatment process 1 of embodiment 1 Ganoderma lucidum spore powder

[0136] Preparation of the test solution: Accurately weigh 0.5g of the sample, place it in a 25ml stoppered test tube, add 10mL of ultrapure water, seal it tightly, extract it by ultrasonic for 60min, make up for the weight loss, shake well, take out 2mL in a 2mL centrifuge tube, and put it at 10000r / min centrifuged for 3min, and passed through a 0.22μm microporous aqueous phase filter membrane to obtain the test solution.

[0137] Preparation of the reference substance: take the ergothioneine reference substance, make a solution containing 0.1 mg ergothioneine per 1 mL with ultrapure water, and dilute it into 5 serial concentrations of standard solution.

Embodiment 2

[0138] The pretreatment process 2 of embodiment 2 Ganoderma lucidum spore powder

[0139] Preparation of the test solution: Accurately weigh 0.5g of the sample, place it in a 25ml stoppered test tube, add 10mL of 50% ethanol, seal it tightly, extract it ultrasonically for 60min, make up for the weight loss, shake well, take out 2mL in a 2mL centrifuge tube, Centrifuge at 10000r / min for 3min, and pass through a 0.22μm microporous aqueous phase filter to obtain the test solution.

Embodiment 3

[0140] The pretreatment process 3 of embodiment 3 Ganoderma lucidum spore powder

[0141] Preparation of the test solution: Accurately weigh 0.5g of the sample, place it in a 25ml stoppered test tube, add 10mL of absolute ethanol, seal it up, ultrasonically extract for 60min, make up for the weight loss, shake well, take out 2mL in a 2mL centrifuge tube, and put it at 10000r / min centrifuged for 3min, and passed through a 0.22μm microporous aqueous phase filter membrane to obtain the test solution.

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Abstract

The invention relates to the field of medicine analysis and relates to a method for determining effective components in a ganoderma lucidum spore powder raw material and a product thereof. The invention relates to high performance liquid chromatogram detection of ergothioneine and / or nucleosides components; and the sensitivity is high and the specificity is strong.

Description

technical field [0001] The invention relates to the field of drug analysis, in particular to establishing a method for determining active ingredients in ganoderma lucidum spore powder raw materials and products thereof. Background technique [0002] Ganoderma lucidum (Ganodema lingzhi Sheng H.Wu et al.) is a precious fungus with high medicinal value. Ganoderma lucidum spores are oval germ cells ejected from the cap during the mature growth stage of Ganoderma lucidum. Because Ganoderma lucidum spore powder has immune regulation, Various biological activities such as anti-tumor and anti-inflammation are increasingly used in health care and pharmaceutical products. At present, there are hundreds of Ganoderma lucidum spore powder health food, and the market share is also very high, mainly Ganoderma lucidum spore powder powder and Ganoderma lucidum spore oil capsule. [0003] Due to the wide variety of Ganoderma lucidum spore powder, wide distribution, and long harvesting time, ...

Claims

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Application Information

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IPC IPC(8): G01N30/88
Inventor 唐庆九王金艳冯娜王晨光张劲松张忠刘艳芳周帅唐传红
Owner SHANGHAI ACAD OF AGRI SCI
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