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Method for preparing crude phycobiliprotein and crude polysaccharide from porphyra yezoensis

A technology of laver and phycobiliprotein is applied in the field of preparation of phycobiliprotein and polysaccharide, and can solve the problems of protein denaturation, leftovers, affecting the extraction and activity of phycobiliprotein, etc.

Inactive Publication Date: 2015-12-02
HUAIHAI INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, only 1 kg of dry laver can be obtained after drying 10-13 kg of fresh and wet laver, and the drying process will consume a lot of heat energy and produce waste, and will also cause protein denaturation and affect the extraction and activity of phycobiliprotein

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1, a method for preparing crude phycobiliprotein and crude polysaccharide by using Porphyra zebra, the main steps of the method include:

[0022] (1) Wash, drain, and centrifugally dehydrate the harvested Porphyra variegata to obtain fresh wet laver with a water content of 79.5%; add potassium dihydrogen phosphate-sodium hydroxide buffer solution with a pH of 8.0 to the fresh wet laver , the mass ratio of the buffer solution to the seaweed is 0.8:1; homogenize to a slurry state, and then swell the slurry material at 38° C. for 8 hours to obtain the first material.

[0023] (2) Treat the first material with a colloid mill, then dilute the treated material with an appropriate amount of water and then process it with a high-pressure homogenizer at 75Mpa to obtain the second material; centrifuge the second material to obtain the first supernatant and the first precipitate; add ammonium sulfate to the first supernatant, then stand at 4°C for 10 hours, then centrif...

Embodiment 2

[0025] Embodiment 2, a method for preparing crude phycobiliprotein and crude polysaccharide by using Porphyra zebra, the main steps of the method include:

[0026] (1) Wash, drain, and centrifugally dehydrate the harvested Porphyra variegata to obtain fresh wet laver with a water content of 81.2%; add potassium dihydrogen phosphate-sodium hydroxide buffer solution with a pH of 8.0 to the fresh wet laver , the mass ratio of the buffer solution to the seaweed is 1:1; homogenize to a slurry state, and then swell the slurry material at 42° C. for 10 hours to obtain the first material.

[0027] (2) Treat the first material with a colloid mill, then dilute the treated material with an appropriate amount of water and then process it with a high-pressure homogenizer at 100Mpa to obtain the second material; centrifuge the second material to obtain the first supernatant and the first precipitate; adding ammonium sulfate to the first supernatant, then standing at 10°C for 12 hours, and t...

Embodiment 3

[0029] Embodiment 3, a method for preparing crude phycobiliprotein and crude polysaccharide by using Porphyra zebra, the main steps of the method include:

[0030](1) Wash, drain, and centrifugally dehydrate the harvested Porphyra variegata to obtain fresh wet laver with a water content of 80%; add potassium dihydrogen phosphate-sodium hydroxide buffer solution with a pH of 8.0 to the fresh wet laver , the mass ratio of the buffer solution to the seaweed is 0.9:1; homogenize to a slurry state, and then swell the slurry material at 40° C. for 9 hours to obtain the first material.

[0031] (2) Treat the first material with a colloid mill, then dilute the treated material with an appropriate amount of water and then process it with a high-pressure homogenizer at 85Mpa to obtain the second material; centrifuge the second material to obtain the first supernatant and the first precipitate; adding ammonium sulfate to the first supernatant, then standing at 6°C for 11 hours, and then ...

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PUM

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Abstract

The invention provides a method for preparing crude phycobiliprotein and crude polysaccharide from porphyra yezoensis. The novel method for preparing crude phycobiliprotein and crude polysaccharide simultaneously comprises steps as follows: fresh and wet porphyra is taken as a raw material, harvested porphyra yezoensis is washed, drained and subjected to centrifugal dewatering processing, and mixing swelling, wet-method ultrafine grinding performed by a colloid mill combined homogenizer, salting-out and other procedures are performed. According to the method, drying processing of the fresh and wet porphyra in the preparation process of phycobiliprotein is eliminated, a prepared crude phycobiliprotein product contains 34.3%-38.9% of phycobiliprotein on the dry basis, and the yield of phycobiliprotein ranges from 4.59% to 4.71%. The method is suitable for industrial application, the crude phycobiliprotein product prepared from porphyra can be taken as a preparation raw material of high-purity porphyra phycobiliprotein, and the prepared crude polysaccharide can be taken as a preparation raw material of polysaccharide.

Description

technical field [0001] The invention relates to a method for preparing phycobiliprotein and polysaccharide, in particular to a method for preparing crude phycobiliprotein and crude polysaccharide by using Porphyra zebra. Background technique [0002] Porphyra is a species of Rhodophyta ( Rhodophyta ), Porphyra ( Porphyra ) is the general term for macroalgae and is one of the most economically important algae. Seaweed ( Porphyray zoensis ) and Tan Laver ( Porphyrahaitanensis ) is the two largest cultivated species of laver in China. Seaweed is high in protein, low in fat, rich in mineral elements and multivitamins, and is considered by Chinese medicine as one of the precious natural seaweed health foods. At present, the laver processing products on the market are mainly dried laver cakes, instant seaweed and brewed laver soup, resulting in a single product, low added value and slow updating and upgrading, which restricts the sustainable development and growth of the l...

Claims

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Application Information

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IPC IPC(8): C07K1/30C07K1/14C08B37/00
Inventor 王灵昭苏琳姚兴存盘赛昆杜云建舒留泉吕玲玲梁俊杰张俊杰王淑军
Owner HUAIHAI INST OF TECH
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