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Preparation method and application of prenylated flavonoid compound with anti-breast cancer activity

A technology of prenylated flavonoids and anti-breast cancer, applied in the field of medicine, can solve problems such as no patents or literature reports, and achieve significant economic and social benefits.

Inactive Publication Date: 2015-12-09
HENAN UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The prenylated flavonoids involved in the present invention and their biological activity have not been patented or reported in the literature so far

Method used

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  • Preparation method and application of prenylated flavonoid compound with anti-breast cancer activity
  • Preparation method and application of prenylated flavonoid compound with anti-breast cancer activity
  • Preparation method and application of prenylated flavonoid compound with anti-breast cancer activity

Examples

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Embodiment 1

[0015] In the specific implementation of the present invention, the prenylated flavonoids with anti-breast cancer activity can be extracted from 9 kg of lotus lobularis as raw material, heated and refluxed 3 times with 18 L of ethanol with a volume ratio of 95%, and the extraction temperature is 95°C. The extraction time is 1.5 hours each time, and ethanol is recovered under reduced pressure to obtain extract-like ethanol extract, which is suspended in 3.2L of distilled water, and extracted three times with petroleum ether, dichloromethane, ethyl acetate, and n-butanol successively, 3.2L each time, the time is 1.5 hours; the ethyl acetate extraction part is separated by silica gel column chromatography, and the volume ratio is 100:0, 100:5, 100:7, 100:10, 100:30, 100:50 , 100:70, 100:100, 100:200, 0:100 petroleum ether-acetone mixed solvents for gradient elution, each gradient uses 13L eluent, and the flow rate is 15mLmin -1 , each 500mL is a fraction, collect 260 fractions, a...

Embodiment 2

[0020] In the specific implementation of the present invention, the prenylated flavonoids with anti-breast cancer activity can also be extracted from 6 kg of lotus lobularis by heating and refluxing 3 times with 30 L of ethanol with a volume ratio of 75%, and the extraction temperature is 90° C. , each extraction time is 2 hours, ethanol is reclaimed under reduced pressure to obtain extract-like ethanol extract, suspended in 2L of distilled water, and extracted 3 times with petroleum ether, dichloromethane, ethyl acetate, n-butanol successively, Each time 2L, the time is 2 hours; the ethyl acetate extraction part is separated by silica gel column chromatography, and the volume ratio is 100:0, 100:5, 100:7, 100:10, 100:30, 100:50, 100:70, 100:100, 100:200, 0:100 petroleum ether-acetone mixed solvent for gradient elution, each gradient uses 9.1 eluent, the flow rate is 10mLmin -1 , each 350mL is a fraction, collect 260 fractions, and each fraction is detected and analyzed by sil...

Embodiment 3

[0025] In the specific implementation of the present invention, the prenylated flavonoids with anti-breast cancer activity can also be extracted from 8 kg of lotus lobularis by heating and refluxing 3 times with 24 L of ethanol with a volume ratio of 85%, and the extraction temperature is 92 ° C. , each extraction time is 1.5 hours, and the ethanol is recovered under reduced pressure to obtain a extract-like ethanol extract, which is suspended in 2.8L of distilled water, and extracted 3 times with petroleum ether, dichloromethane, ethyl acetate, and n-butanol successively , 2.8L each time, the time is 1.5 hours; the ethyl acetate extraction part is separated by silica gel column chromatography, and the volume ratio is 100:0, 100:5, 100:7, 100:10, 100:30, 100: 50, 100:70, 100:100, 100:200, 0:100 petroleum ether-acetone mixed solvent for gradient elution, each gradient uses 11.7L eluent, the flow rate is 13mLmin -1 , each 450mL is a fraction, collect 260 fractions, and each frac...

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Abstract

The invention relates to a preparation method and application of a prenylated flavonoid compound with anti-breast cancer activity, and can effectively solve the problem about preparation of anti-hepatoma medicine through preparation of the prenylated flavonoid compound with anti-breast cancer activity. The method comprises the following steps: extracting fructus podophylli with ethyl alcohol; recycling ethyl alcohol; suspending in distilled water; extracting with petroleum ether, dichloromethane, ethyl acetate and n-butyl alcohol; carrying out gradient elution on ethyl acetate extraction parts with petroleum ether-acetone; combining flow parts to obtain components Fr.1-Fr.16; eluting component Fr.4 with methyl alcohol, and eluting the obtained sub-component Fr.4-1 with methyl alcohol-water, so as to obtain sinoflavonoid O; eluting component Fr.5 with methyl alcohol, and eluting the obtained sub-component Fr.5-1 with methyl alcohol-water, so as to obtain sinoflavonoid J, sinoflavonoid K, sinoflavonoid M and sinoflavonoid N; eluting component Fr.6 with methyl alcohol, eluting the obtained sub-component Fr.6-1 with methyl alcohol-water, and eluting the obtained sub-component Fr.6-1-2 with methyl alcohol-water, so as to obtain sinoflavonoid L. The prenylated flavonoid compound has anti-breast cancer activity, and is used for preparing anti-breast cancer medicine.

Description

technical field [0001] The invention relates to medicine, in particular to a preparation method and application of prenylated flavonoids with anti-breast cancer activity. Background technique [0002] Malignant tumor is a common and frequently-occurring disease that seriously threatens human health and life. Today, cancer is the leading cause of death worldwide. Breast cancer is a malignant tumor that usually occurs in the glandular epithelial tissue of the breast. It has become one of the major diseases that seriously threaten the health and life of young and middle-aged women. The number of newly diagnosed breast cancer is more than 1 million. Although there are many drugs for the treatment of breast cancer at present, all of them have strong toxic and side effects. Among them, the reduction of digestive function and the inhibition of bone marrow hematopoietic function are the most obvious. It often makes it difficult for breast cancer patients to persist in completing t...

Claims

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Application Information

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IPC IPC(8): C07D311/30C07D311/58C07D493/04A61P35/00A61P15/14
CPCC07D311/30C07D311/58C07D493/04
Inventor 孙彦君裴莉昕司金光纪宝玉王俊敏高美玲郝志友张艳丽陈辉
Owner HENAN UNIV OF CHINESE MEDICINE
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