Preparation and application of Prussian blue/N-doped carbon nanometer composite material

Abstract

The invention discloses preparation and application of a Prussian blue / N-doped carbon nanometer composite material to the aspect of enzyme-free uric acid sensors. A preparation method of the Prussian blue / N-doped carbon nanometer composite material comprises the following three steps: acidizing treatment of a carbon nanometer composite material; preparation of a N-doped carbon nanometer composite material; preparation of the Prussian blue / N-doped carbon nanometer composite material. The prepared Prussian blue / N-doped carbon nanometer composite material is utilized for preparation of an enzyme-free uric acid sensor, has a very good three-dimensional space structure and a very good effect in catalyzing uric acid, and can be utilized for directly detecting uric acid under the circumstance that uricase is absent; compared with a common uricase sensor, the enzyme-free uric acid sensor prepared by utilizing the Prussian blue / N-doped carbon nanometer composite material is simpler, more economical and portable, long in storage period, and high in stability and anti-interference capability.

Description

technical field [0001] The invention belongs to the fields of energy material preparation and electrochemistry, and in particular relates to the preparation of a Prussian blue / N-doped carbon nanocomposite material and its application in an enzyme-free uric acid sensor. Background technique [0002] Uric acid (UA) is the main product of purine metabolism in the human body. Excessive or low levels of uric acid in the human body are not conducive to health. For example, excessive levels of uric acid in serum can lead to hyperuricemia; it can also damage kidney function and cause uric acid excretion disorders. Relevant data show that the increase of blood uric acid is related to hypertension, diabetes, coronary heart disease and other diseases. As a reducing substance, uric acid participates in some important redox reactions in the body. At present, with the development of society, due to many factors such as work stress and overnutrition, the probability of people suffering f...

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Problems solved by technology

[0003] The traditional uric acid sensor is mainly an enzyme sensor based on uricase, but the enzyme itself has some disadvantages: (1) the activity of the enzyme, the shelf life of the enzyme is limited, and its activity is easily affected by temperature and pH; (2) the enzyme In order to fix the enzyme on the electrode, it is necessary to find a biocompatible material that will not affect the activity of the enzyme; (3) the price of the enzyme, the price of the enzyme is relatively expensive, which will make the cost of the sensor relatively high; (4) The problem of the amount of enzyme immobilization, the amount of enzyme that can be immobilized on the electrode is limited

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