Methods for reducing one or more tobacco specific nitrosamines in tobacco material

A technology for nitrosamines and tobacco, which is applied in the directions of tobacco, analysis materials, and tobacco processing, and can solve the problems of deteriorating tobacco quality, insoluble matrix binding, etc.

Active Publication Date: 2015-12-09
PHILIP MORRIS PROD SA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the treatment can introduce toxicologically relevant compounds into the tobacco and significantly deteriorate the quality of the tobacco
Matrix-bound form not readily soluble using pH-neutral aqueous extraction methods

Method used

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  • Methods for reducing one or more tobacco specific nitrosamines in tobacco material
  • Methods for reducing one or more tobacco specific nitrosamines in tobacco material
  • Methods for reducing one or more tobacco specific nitrosamines in tobacco material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0109] Method for analyzing free and bound NNK in tobacco

[0110] An aliquot (eg, about 750 mg) of the tobacco sample was extracted with about 30 mL of Tris-HCl buffer (50 mM; pH 7.4) by shaking at about room temperature for about 1 hour. Add internal standard (100ng / mLNNK-d 4 ). A sample (0.4 mL) of the extract was filtered using a 0.2 μΜ filter and analyzed for NNK content using ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS / MS). The sample concentrations calculated from these extract concentrations correspond to the "free NNK" concentrations in the samples. After processing the extraction mixture (eg, by heating to about 130° C. for about 4 hours) and filtering an aliquot of the extract, the NNK concentration was again measured by UPLC-MS / MS. From these values, the "total NNK" concentration in the sample can be calculated. The "bound NNK" concentration is the difference between the "total NNK" and "free NNK" concentrations.

[0111] An alter...

Embodiment 2

[0113] UPLC analysis

[0114] The column used was Waters Acquity BEHC18, 1.7 μm, 2.1×50 mm. The eluents used were: (A) ammonium bicarbonate (10 mM; adjusted to pH 9.8 with ammonia) + 2% (v / v) acetonitrile; (B) acetonitrile. The gradient used was 0 min-5%B; 0.5min-5%B; 3.3min-18.3%B. The flow rate used was 0.5 mL / min. The column temperature used was 50°C.

Embodiment 3

[0116] MS / MS methodology

[0117] The analysis was performed on a Waters TQ spectrometer using the following MRM transitions: NNK: 208.2 ◇ 122.2; dwell time 100 ms; NNK-d4: 212.2 ◇ 126.2; dwell time 100 ms; capillary voltage: 0.6 kV; cone voltage: 25 V; collision energy : 11eV; source temperature: 120°C; desolvation temperature: 400°C; desolvation gas flow: 800L / h.

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Abstract

There is described herein a method of reducing the amount of at least matrix-bound NNK in tobacco material comprising the steps of: (a) providing tobacco material comprising at least matrix-bound NNK; (b) optionally measuring the level of at least matrix-bound NNK in the tobacco material; (c) heating the tobacco material for at least about 30 seconds to a temperature of greater than about 110 degrees Celsius in the presence of a liquid or steam to release at least a portion of the matrix-bound NNK from the insoluble tobacco matrix of the tobacco material; (d) optionally measuring the level of at least matrix-bound NNK in the tobacco material following step (c); (e) optionally comparing the levels of matrix-bound NNK obtained in steps (b) and (d); and (f) identifying tobacco material in which at least matrix- bound NNK has been removed from the tobacco material.

Description

technical field [0001] The present invention relates to methods for reducing the amount of one or more types of tobacco-specific nitrosamines, including 4-(methylnitrosamino)-1-(3-pyridyl), in tobacco material - 1-butanone (NNK). A tobacco product comprising tobacco material obtained or obtainable by the method is also described. Background technique [0002] During the manufacture and processing of tobacco products, by-products such as tobacco stalks, loose leaves and tobacco dust are generated during the manufacturing process including stemming, aging, blending, cutting, drying, cooling, screening, shaping and packaging , and the by-products can be recycled to recover their useful tobacco content. For example, tobacco stem and tobacco shreds obtained from the manufacturing process are not suitable for direct use in the manufacture of tobacco products. Since the stalks and shreds represent a significant amount of raw material investment, methods have been developed to fu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A24B15/24
CPCA24B15/245A24B15/12G01N33/4833A24B15/24
Inventor G·郎I·京迪兹A·维亚尔诺兹-比塞
Owner PHILIP MORRIS PROD SA
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