Preparation of a polysaccharide synbiotic from Yupingfeng dregs and its application in immune regulation
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A technology of Yupingfeng and synbiotics, which is applied in the fields of application, drug combination, and medical preparations containing active ingredients, and can solve problems such as unseen
Active Publication Date: 2018-06-08
SICHUAN AGRI UNIV
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However, there are few reports of polysaccharide synbiotics from traditional Chinese medicine residues, and no related products
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Embodiment 1
[0020] Example 1 Identification of Rhizopus oligosporus SH
[0021] The genome total DNA of mold SH was extracted according to the modified benzyl chloride method of (Zhu H, 1993.Nucleic Acids Research, 21 (22): 5279), and the total DNA was detected by 2% agarose gel ( figure 1 ). The ITS1-5.8S-ITS2 region of the total DNA of the mold SH genome was amplified according to the method of (Liu Xiaoli. 2009. Modern Food Science and Technology, 25 (5): 2578-2585), and the PCR product was detected with 1.0% agarose gel ( figure 2 ). The purified PCR product was sent to Beijing BGI for sequencing, and the sequence length of the mold SHITS1-5.8S-ITS2 region was determined to be 651bp (sequence table 1). After carrying out the Blast comparison in Genbank, it has the highest affinity with Rhizopus oligosporum, and the homology reaches 100%, and a phylogenetic tree is established ( image 3 ). Therefore, the present invention identified it as Rhizopus oligosporus (Rhizopus oligosporu...
Embodiment 2
[0022] The domestication of embodiment 2 Rhizopus oligospore SH
[0023] Inoculate Rhizopus oligosporus SH into PDA containing 5% YPD, culture at 28°C for 24 hours, pass for 5 generations, and then inoculate into PDA culture solution containing 10%, 20%, 40%, 80%, and 100% YPD in sequence Cultured in medium for 24 hours, each concentration was continuously passed for 3 generations, and Rhizopus oligospora SH adapted to YPD was obtained, which was stored for future use.
Embodiment 3
[0024] Example 3 Preparation of polysaccharide synbiotics from Yupingfeng herbal dregs
[0025] Dissolve YPD in distilled water (final concentration: 0.1g / mL), sterilize with flowing steam for 60min, cool down, inoculate purified and domesticated Rhizopus oligospore SH with 3% inoculum size, 150rpm, 28°C constant temperature fermentation for 2-3 days , Vacuum freeze-drying into powder is Synbiotics. Synbiotics were taken to make a solution with a final concentration of 1g / mL, sterilized by a 0.22μm syringe filter, and stored at 4°C for later use.
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Abstract
The invention discloses a preparation method of polysaccharide synbiotics from Yupingfeng dregs and its application in immune regulation. The synbiotic is prepared by fermenting Yupingfeng dregs polysaccharide (YPD) by Rhizopus oligospore SH, and belongs to the field of resource utilization technology of traditional Chinese medicine dregs and microecological preparations. The gist of the invention is to use YPD as the only raw material to obtain the polysaccharide synbiotics from Yupingfeng dregs through SH fermentation of Rhizopus oligosporus, including YPD dissolution, circulation steam sterilization, cooling, inoculation, fermentation, freeze-drying and other links. The synbiotic significantly improved the in vitro proliferation activity, phagocytosis activity and NO release ability of mouse lymphocytes and macrophages; and could up-regulate intracellular IL‑6, TNF‑α, IFN‑γ, NF‑κB, TLR‑ 4 and iNOS expression; while in the LPS-induced chronic inflammation model, synbiotics significantly inhibited the in vitro proliferation of lymphocytes, and significantly down-regulated intracellular IL‑6, TNF‑α, IFN‑γ, NF‑κB, TLR‑ 4 and expression of iNOS. It shows that polysaccharide synbiotics from Yupingfeng medicinal dregs have two-way immunoregulatory effect and can be used as an immunomodulator.
Description
technical field [0001] The invention relates to the preparation of polysaccharide synbiotics from Yupingfeng medicinal dregs and its application in immune regulation, and belongs to the field of resource utilization technology of traditional Chinese medicine dregs and microecological preparations. Background technique [0002] Traditional Chinese medicine has been widely used in the prevention and treatment of various diseases due to its low toxicity and side effects, and has a long history in Asian countries. In recent years, both commercial demand and academic research, traditional Chinese medicine has received widespread attention, and the syndrome differentiation theory of traditional Chinese medicine has gradually gained popularity. A large number of studies have reported that many traditional Chinese medicines have immunomodulatory functions, but there are few studies on traditional Chinese medicine residues. With the rapid development of the Chinese medicine industry...
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