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Cordyceps sinensis liquid state fermentation culture medium for producing exopolysaccharides

An extracellular polysaccharide, liquid fermentation technology, applied in microorganism-based methods, fermentation, microorganisms, etc., can solve the problems of low yield of extracellular polysaccharide and insignificant effect, and achieve the effect of increasing yield, low cost and promoting growth.

Inactive Publication Date: 2015-12-16
TIANJIN MODERN VOCATIONAL TECH COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to excessive collection, wild Cordyceps resources are scarce in nature, and Cordyceps polysaccharides, cordycepic acid and cordycepin are the main active substances of Cordyceps sinensis. According to pharmacological and clinical studies, Cordyceps polysaccharides can enhance immunity, anti-tumor and lower blood sugar. For the research and production of Cordyceps polysaccharides, most of them focus on the extraction, separation and purification of fruit bodies and mycelia, and there are few studies on the production of extracellular polysaccharides from Cordyceps sinensis in liquid state. The methods for obtaining extracellular polysaccharides from Cordyceps sinensis in the prior art include: Two types: (1) Cordyceps sinensis is cultured in a common liquid medium to obtain exopolysaccharides, but the exopolysaccharide yield is low
(2) Increase the production of exopolysaccharide by changing the process of Cordyceps sinensis liquid fermentation, but the effect is not obvious

Method used

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  • Cordyceps sinensis liquid state fermentation culture medium for producing exopolysaccharides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] (1) Preparation of liquid medium: by mass percentage, it includes glucose 3%, peptone 1.5%, yeast powder 0.5%, KH 2 PO 4 0.3%, MgSO 4 ·7H 2 O0.15% and potato 20%, the solvent is water, and the pH value of the liquid medium is 6.0;

[0029] (2) Fermentation culture: put the prepared fermentation medium into a fermenter with a capacity of 1 L, sterilize, inoculate the strains into the liquid medium under aseptic conditions, and cultivate at a constant temperature at 24°C and 150r / min;

[0030] (3) After the fermentation finishes, use a microscope to observe the growth of mycelia in the fermented liquid, and inoculate the fermented liquid on the nutrient agar plate to check whether it is infected with bacteria;

[0031] (4) Centrifuge the uninfected fermentation broth detected in step (3) at 4000r / min for 20min to obtain the fermented mycelia in the form of pellets, and then freeze-dry the mycelium and supernatant and stored in a -18°C refrigerator for later use;

[0...

Embodiment 2

[0036] (1) Preparing a nutrition package, the mixed nutrition package includes by mass percentage: 31.1% histidine, 31.1% lysine, 18.3% glutamine, 18.3% vitamin B2 and 1.2% selenium;

[0037] (2) Preparation of liquid medium: a medium for the production of exopolysaccharides by liquid fermentation of Cordyceps sinensis comprising: 0.02% mixed nutrition package, 3% glucose, 1.5% peptone, 0.5% yeast powder, KH 2 PO 4 0.3%, MgSO 4 ·7H 2 O0.15% and potato 20%, the solvent is water, and the pH value of the liquid medium is 6.0;

[0038] (3) Fermentation culture: put the prepared fermentation medium into a fermenter with a capacity of 1 L, sterilize, inoculate the strains into the liquid medium under aseptic conditions, and cultivate at a constant temperature at 24°C and 150r / min;

[0039] (4) After the fermentation finishes, use a microscope to observe the growth of mycelia in the fermented liquid, and inoculate the fermented liquid onto the nutrient agar plate to check whether ...

Embodiment 3

[0045] (1) Preparing a nutrition package, the mixed nutrition package includes by mass percentage: 31.1% histidine, 31.1% lysine, 18.3% glutamine, 18.3% vitamin B2 and 1.2% selenium;

[0046] (2) Preparation of liquid medium: a medium for the production of exopolysaccharides by liquid fermentation of Cordyceps sinensis, including: 0.04% mixed nutrition package, 3% glucose, 1.5% peptone, 0.5% yeast powder, KH 2 PO 4 0.3%, MgSO 4 ·7H 2 O0.15% and potato 20%, the solvent is water, and the pH value of the liquid medium is 6.0;

[0047] (3) Fermentation culture: put the prepared fermentation medium into a fermenter with a capacity of 1 L, sterilize, inoculate the strains into the liquid medium under aseptic conditions, and cultivate at a constant temperature at 24°C and 150r / min;

[0048] (4) After the fermentation finishes, use a microscope to observe the growth of mycelia in the fermented liquid, and inoculate the fermented liquid onto the nutrient agar plate to check whether ...

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Abstract

The invention provides a cordyceps sinensis liquid state fermentation culture medium for producing exopolysaccharides. The culture medium is prepared from 1-5% by mass of glucose, 1-4% by mass of peptone, 0.5-3% by mass of yeast powder, 0.1-0.5% by mass of KH2PO4, 0.1-0.5% by mass of MgSO4.7H2O, 5-40% by mass of potato lumps and a mixed nutrition bag, a solvent is water and a PH value of the liquid culture medium is 6.0. The cordyceps sinensis liquid state fermentation culture medium can promote cell growth of the cordyceps sinensis and the secretion of exopolysaccharides, the yield of exopolysaccharides obtained by culturing in a 1-L quadruple fermentation tank for 72 hours is up to 8.18g / L, and the content of the exopolysaccharides obtained by culturing the cordyceps sinensis is improved by 76% compared with the prior art.

Description

technical field [0001] The invention relates to the field of bioengineering, in particular to a culture medium for producing exopolysaccharides by liquid fermentation of Cordyceps sinensis. Background technique [0002] Cordyceps sinensis is a complex of the subseapots and larvae corpses of the ergot fungus Cordyceps sinensis parasitizing on the larvae of the bat moth family insects. However, due to excessive collection, wild Cordyceps resources are scarce in nature, and Cordyceps polysaccharides, cordycepic acid and cordycepin are the main active substances of Cordyceps sinensis. According to pharmacological and clinical studies, Cordyceps polysaccharides can enhance immunity, anti-tumor and lower blood sugar. For the research and production of Cordyceps polysaccharides, most of them focus on the extraction, separation and purification of fruit bodies and mycelia, and there are few studies on the production of extracellular polysaccharides from Cordyceps sinensis in liquid ...

Claims

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Application Information

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IPC IPC(8): C12P19/04C08B37/00C12R1/645
Inventor 殷海松汤卫华陈珊张乐牛红军王立晖刘鑫龙张轶斌龙尾苑鹏刘鹏潘志恒黄艳玲
Owner TIANJIN MODERN VOCATIONAL TECH COLLEGE
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