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Method for detecting cosmetic and raw materials of cosmetic

A detection method and technology of raw materials, applied in material inspection products, biological testing and other directions, can solve the problems of safety risks and difficult to repeat testing, and achieve the effect of easy repeated testing, reducing safety risks, and real experiments.

Inactive Publication Date: 2015-12-16
广州艾卓生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] This application provides a detection method for cosmetics, which solves the problems in the prior art that human body is not the test object for cosmetics detection, there are safety risks and it is not easy to repeat the test

Method used

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  • Method for detecting cosmetic and raw materials of cosmetic
  • Method for detecting cosmetic and raw materials of cosmetic
  • Method for detecting cosmetic and raw materials of cosmetic

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Take 10 chicken embryos that have been hatched for 10 days, and use a hand twist drill to make a small hole on the air cell end of the chicken embryo, and then use serrated tweezers to carefully expand the small opening on the chicken embryo, and finally expand the opening to the entire air cell area. Then, the saline was dripped all over the end of the air cell to fully wet the air cell membrane, and then the air cell membrane was carefully torn off with serrated tweezers to expose the CAM of the chicken embryo chorioallantoic membrane.

[0032] The tetrafluoro ring 20 is pasted and fixed on the CAM, and the part of the CAM inside the tetrafluoro ring 20 forms the test area 11 . The pomegranate facial mask is made into a transparent suspension with sterile water, and a certain amount of the suspension is added to the test area 11. Then cover the outside of the chicken embryo with a layer of plastic wrap to prevent the water loss of the chicken embryo, and place the chi...

Embodiment 2

[0035] Take 5 chicken embryos that have been hatched for about 10 days, and use a hand twist drill to make a small hole on the end of the air cell of the chicken embryo, and then use serrated tweezers to carefully expand the small opening on the chicken embryo, and finally expand the opening to the entire air cell area. Then, the saline was dripped all over the end of the air cell to fully wet the air cell membrane, and then the air cell membrane was carefully torn off with serrated tweezers to expose the CAM of the chicken embryo chorioallantoic membrane.

[0036] The tetrafluoro ring 20 is pasted and fixed on the CAM, and the part of the CAM inside the tetrafluoro ring 20 forms the test area 11 . Use sterile water to make a transparent suspension of "Pechoin Moisturizing and Brightening Essence Mask", and add a certain amount of the suspension to the test area 11. Then cover the outside of the chicken embryo with a layer of plastic wrap to prevent the water loss of the chick...

Embodiment 3

[0039] Take 5 chicken embryos that have been hatched for about 10 days, and use a hand twist drill to make a small hole on the end of the air cell of the embryo, and then use serrated tweezers to carefully expand the small opening on the embryo, and finally expand the opening to the entire air cell area. Then, the saline was dripped all over the end of the air cell to fully wet the air cell membrane, and then the air cell membrane was carefully torn off with serrated tweezers to expose the CAM of the chicken embryo chorioallantoic membrane.

[0040] The tetrafluoro ring 20 is pasted and fixed on the CAM, and the part of the CAM inside the tetrafluoro ring 20 forms the test area 11 . Make a transparent suspension of "Pechoin Eight Cups of Water Moisturizing Mask" with sterile water, and add a certain amount of the suspension to the test area 11. Then cover the outside of the chicken embryo with a layer of plastic wrap to prevent the water loss of the chicken embryo, and place t...

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Abstract

The invention discloses a method for detecting a cosmetic and raw materials of the cosmetic. The method includes the following steps that a plurality of animal fertilized egg embryos are taken, an opening is formed in the air chamber end of each egg shell, normal saline is added into the openings, and air chamber membranes are torn to expose chorioallantoic membranes; teflon rings are fixedly attached to the chorioallantoic membranes of the animal fertilized egg embryos, and tested regions are formed by in-ring parts of the teflon rings; a plurality of to-be-tested samples are taken and added to the tested regions, then the outer sides of the animal fertilized egg embryos are covered with preservative films, and then the animal fertilized egg embryos are arranged in an incubator; after a period of time, the animal fertilized egg embryos are taken out of the incubator, changes of blood vessels of the in-ring chorioallantoic membranes and changes of blood vessels of the out-ring chorioallantoic membranes are observed and compared, injury grades are determined, and stimulation grades of the to-be-tested samples are determined according to the injury grades. According to the method, the human body is not treated as an experimental subject, safety risks are reduced, and repeated testing is convenient.

Description

technical field [0001] The application relates to the technical field of cosmetics, in particular to a detection method for cosmetics and raw materials thereof. Background technique [0002] At this stage, consumers or testing agencies conduct skin irritation tests on cosmetics and their raw materials to test the quality of cosmetics. This test is typically a skin patch test, which evolved from the allergen screening test in medical testing. The skin patch test is widely used in the allergy testing of cosmetics and their raw materials because of its simple experiment and wide application range. [0003] The specific method is: Randomly search for more than 10 test subjects, who are required to be healthy adults, with no history of special allergies, no history of major diseases, no gender limitation, and mainly women. The inner forearm and upper part of the wrist of the subject were repeatedly washed with physiological saline. Apply 0.5ml of the test substance evenly on...

Claims

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Application Information

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IPC IPC(8): G01N33/48
Inventor 林浩秀刘文静何慧玲冼宝仪张军张晓琮
Owner 广州艾卓生物科技股份有限公司
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