Application of miR-375-3p in prevention and treatment of lepidoptera pests

A technology of Lepidoptera insects and insects, applied in the fields of application, insecticides, animal repellents, etc., can solve the problems that have not been reported

Active Publication Date: 2015-12-23
SOUTH CHINA NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, important miRNAs in the relationship between insects and plants have not been reported

Method used

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  • Application of miR-375-3p in prevention and treatment of lepidoptera pests
  • Application of miR-375-3p in prevention and treatment of lepidoptera pests
  • Application of miR-375-3p in prevention and treatment of lepidoptera pests

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 1. miR-375-3p treatment affects the growth of Spodoptera litura

[0026] Five-instar first-day Spodoptera litura larvae with the same size and health status were selected, weighed each larva, and randomly divided into miR-375-3p treatment group and NC control group, 40 larvae in each group, and repeated 3 times. Following the direction of blood circulation, inject artificially synthesized miR-375-3p with a microinjector between the first and second gastropods of the flank of Spodoptera litura larvae, and the sequence is 5'-UUUGUUCGCCCCGGCUCGAGUCG-3' (SEQ ID NO: 1) and NC: 5'-UUCUCCGAACGUGUCACGU-3' (SEQ ID NO: 2), a known nonsense small molecule fragment that does not act on any gene; the injection amount was 4 μg per worm, and fed mustard greens after injection. On the 1st, 2nd, and 3rd day after injection, the morphological changes of the insects were observed and photographed for evidence, and the mortality of the insects was counted every day.

[0027] Table 1 Mor...

Embodiment 2

[0043] Effect of embodiment 2 Sl-miR-375-3p on the growth and development of silkworm

[0044] Since miR-375-3p is conserved in Lepidoptera insects, does Spodoptera litura Sl-miR-375-3p also affect the growth and development of other Lepidoptera insects? For this reason, the 5th instar larvae of the silkworm were subjected to an injection experiment similar to "1" in Example 1. After the injection, the morphological changes of the worms were observed and evidence was taken, and the mortality of the worms was counted every day.

[0045] Test results such as Figure 6 As shown, it can be seen that 100% of the silkworms injected with 2ug Spodoptera litura Sl-miR-375-3p grew abnormally, and the worms were thin and small. After 72 hours, the mortality rate reached 54% (see Table 3).

[0046] Table 3 Mortality statistics of silkworm injected with Spodoptera litura miR-375-3p

[0047]

[0048] The following compares the Sl-miR-375-3p sequence of Spodoptera litura with the miR-...

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Abstract

The invention discloses application of miR-375-3p in prevention and treatment of lepidoptera pests. According to the application, prodenia litura which are agricultural pests are adopted to do an experiment which shows that Sl-miR-375-3p affects intake of polypide due to suppression of the expression of detoxifying enzymes and metabolizing enzymes which are required for digestion of food in the polypide, and finally thinness and smallness and even death of the polypide are caused. The prodenia litura miR-375-3p can be used for preventing and treating pests of any lepidoptera insects; by the same reasoning, the miR-375-3p of any lepidoptera pests can be used for preventing and treating the pests of the same type of other insects. The miR-375-3p of the insects can be used for preparing an insecticide or preventing and treating the pests of transgenic plants expressing the miR-375-3p.

Description

technical field [0001] The present invention relates to the application of miR-375 in the control of Spodoptera litura, specifically relates to the function of miR-375-3p inhibiting Spodoptera litura anti-phytotoxin mechanism so as to achieve high-efficiency insecticide, so as to carry out transgenic or inhibit miR-375-3p The research and development of pesticides can achieve the purpose of efficient pest control. Background technique [0002] microRNA (miRNA) is a class of endogenous non-coding RNA discovered in the 1990s (LeeRC, FeinbaumRL, AmbrosV.TheC.elegansheterochronicgenelin-4encodessmallRNAswithantisensecomplementaritytolin-14.Cell, 1993,75(5):843-54.), About 22 nucleotides. Their biosynthesis is transcribed by RNA polymerase II (RNA polymerase II, pol II) to form primary transcripts (pri-miRNA) with a length of hundreds or even thousands of bases, where the 5' end of the pri-miRNA has a cap structure and poly (A) Tail. pri-miRNA is cleaved under the action of RN...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N57/16A01P7/04
Inventor 郑思春邹晓鹏邱彬彬
Owner SOUTH CHINA NORMAL UNIVERSITY
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