Single cell array chip based on fluid dynamics

Abstract

The invention discloses a single cell array chip based on fluid dynamics, and belongs to the technical field of cell detection. The chip is formed by bonding a glass substrate and polydimethylsiloxane with a flow channel. The flow channel comprises a main channel, grooves and slits, the main channel is a snakelike flow channel, one groove and one slit form an auxiliary channel, the groove in each auxiliary channel is communicated with the corresponding slit, adjacent linear flow channel sections of the main channel are communicated through the auxiliary channels, and the auxiliary channels are arranged in parallel between the adjacent linear flow channel sections of the main channel to form a groove and slit array. For Jurkat cells, a single cell array with the cell occupancy and the single cell rate over 90% can be obtained, and the obtained living cell rate in the grooves is over 98%; the single cell array can be obtained fast to achieve the optimized single cell graphical effect; biochemistry characteristic analysis can be directly carried out on cells in the chip, observation is easy, and heterogeneity of the single cells can be detected.

Description

technical field [0001] The invention belongs to the technical field of cell detection, in particular to a single-cell array chip based on fluid dynamics. Background technique [0002] The data obtained by traditional cell detection methods are based on the average value of a large number of cells, and the specific information of a single cell obtained by traditional cell detection methods is often considered "wrong" or "noise" and ignored or discarded. In modern biomedical research, especially in tumor research, due to the heterogeneity of cells, traditional cell detection methods often cannot meet the requirements for accurate analysis of cell signals. Single-Cell Analysis (Single-Cell Analysis) is an analysis method that focuses on the study of the unique response of a single cell to different stimuli; this analysis method overcomes the shortcomings of traditional cell detection methods, and is suitable for the study of single-cell individuals and the early stages of disea...

AI Technical Summary

Problems solved by technology

[0004] The present invention proposes a single-cell array chip based on fluid dynamics, which can overcome the relatively low throughput in the existing single-cell research based on hydrodynamic characteristics; there is a potential impact on cells (shear stress, etc.); High processing accuracy requirements; more cell loss and short duration (usually less than 24h); for Jurkat cells, a single cell array with a cell occupancy rate and a single cell rate of more than 90% can be obtained, and the activity in the groove can be obtained. The cell rate exceeds 98%; the chip has a simple structure and is less difficult to manufacture, and can quickly realize single-cell arraying and achieve the best single-cell patterning effect; Analyze the biochemical characteristics of the cells, easy to observe

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