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Application of a strain of Bacillus lysinus globosa and its crystal protein and active product

A lysine bacillus, lysine spore technology, applied in the direction of peptide/protein components, microorganism-based methods, medical preparations containing active ingredients, etc. The morphological characteristics of crystal toxins are not mentioned, and the effect of broad-spectrum anti-tumor activity is achieved

Active Publication Date: 2019-04-16
HUNAN NORMAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

People's research on this kind of bacteria is mainly focused on killing insects, but there are very few studies on anti-tumor, and the report only shows that the soluble crystalline toxin extracted from Bacillus sphaericus IAB872 has anti-tumor activity on A549 cancer cells, but does not mention its anti-tumor activity. The active metabolites in the fermentation broth have broad-spectrum antitumor activity, and the specific morphological characteristics of crystal toxins are not mentioned

Method used

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  • Application of a strain of Bacillus lysinus globosa and its crystal protein and active product
  • Application of a strain of Bacillus lysinus globosa and its crystal protein and active product
  • Application of a strain of Bacillus lysinus globosa and its crystal protein and active product

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Embodiment 1

[0047] Bacillus sphaericus of the present invention is Bacillus sphaericus BsX050, Lysinibacillus sphaericus BsX050 was deposited in China Center for Type Culture Collection (CCTCC for short, address: Wuhan University, Wuhan, China) on April 24, 2014, and the deposit number is CCTCC NO: M 2014160.

[0048] (1) The specific isolation process of the strain: collect soil samples from Yuelu Mountain in Changsha, add sterile water, mix well, heat at 75°C for 30min, take an appropriate amount of homogenate and spread it on an LB plate, and incubate at 30°C for 48 hours. Pick a single colony from the plate, dilute it again and streak it on the LB plate, culture it at 30°C for 24 hours, inoculate the single colony obtained by repeated separation and purification into the LB liquid medium shake flask, culture it at 30°C, 120rpm, for 12 hours, Mix with 50% glycerol and store in -80°C refrigerator for a long time.

[0049] Gram staining process: take a little bacterial solution, smea...

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Abstract

The invention discloses Lysinibacillus sphaericus and application of crystal protein and an active product of the Lysinibacillus sphaericus. The Lysinibacillus sphaericus is Lysinibacillus sphaericus BsX050, and is collected in China Center for Type Culture Collection, and the collection number of the bacillus subtilis is CCTCC NO:M 2014160. The invention also discloses the application of the crystal protein and an active product of the Lysinibacillus sphaericus in antitumor therapy. The crystal protein of the Lysinibacillus sphaericus has obvious toxicity on breast cancer cells and hepatoma carcinoma cells of mice, but has no obvious toxicity on human umbilical vein endothelial cells, and the active metabolite has broad-spectrum antitumor activity.

Description

technical field [0001] The present invention relates to a strain of Bacillus lysinus globosa and its crystal protein and active product isolation method and application, in particular to a highly effective anti-tumor wild-type strain ( Lysinibacillus sphaericus BsX050) and its active metabolite separation method and anti-tumor application, and its crystal protein anti-tumor application. Background technique [0002] In recent years, Bacillus ( Bacillus ) as a group of microorganisms that can produce abundant metabolites has attracted increasing attention. Most of Bacillus can secrete highly active extracellular products, such as protease, amylase, glucanase, cellulase and other enzymes. Bacillus is widely used as probiotics in pest control, inhibition of some pathogenic bacteria and anti-tumor activity research. [0003] With the change of human disease spectrum, malignant tumor has become one of the top three causes of death of human beings, seriously endangering human ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P21/00C07K14/195C07K1/36C07K1/34C07K1/30A61K38/16A61P35/00C12R1/01
Inventor 丁学知夏立秋王婷孙运军胡胜标余子全黄伟涛
Owner HUNAN NORMAL UNIVERSITY
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