Polypeptide and application thereof
A peptide synthesis and sequence technology, applied in the direction of application, peptide, peptide source, etc., can solve the problems that are difficult to achieve effective control and complete cure, drug resistance reduces the growth performance of pigs, etc., achieves huge medicinal development potential, promotes The effect of uterine wound healing and improving the economic benefits of pig farms
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Embodiment 1
[0042] (1) Take the natural plant Panax notoginseng and chop it up, homogenate it, add 0.06moL / L Tris-Hcl buffer solution with a pH of 8.0 equivalent to 8 times the volume of the plant raw material, sonicate for 4min, centrifuge to remove the slag, and take the supernatant solution, to obtain the crude protein extract of Panax notoginseng root;
[0043] (2) Precipitate the crude notoginseng root protein extract obtained in step (1) with ammonium sulfate, place it at 4°C overnight, then centrifuge, take the precipitate, and freeze-dry to obtain the notoginseng root protein coarse powder;
[0044] (3) Take notoginseng root protein powder, dissolve it in distilled water, adjust the pH to 8.0 with 5 mol / L NaOH solution, add a compound enzyme composition equivalent to 0.5‰ of vegetable protein meal weight, and the compound enzyme composition Mix alkaline protease and neutral protease at a mass ratio of 1:2; place in a constant temperature water bath at 50°C for 3 hours of enzymolys...
Embodiment 2
[0052] (1) Mince the aloe vera leaves, homogenate, add 0.06moL / L Tris-Hcl buffer solution with a pH of 8.5 equivalent to 6 times the volume of the plant material, sonicate for 3.5min, centrifuge to remove slag, and take the supernatant liquid, that is, the crude extract of aloe vera leaf protein;
[0053] (2) Precipitate the crude aloe leaf protein extract obtained in step (1) with ammonium sulfate, place it at 4°C overnight, then centrifuge, take the precipitate, and freeze-dry to obtain aloe leaf vegetable protein powder;
[0054] (3) Take the aloe vera leaf protein coarse powder obtained in step (2), dissolve it with distilled water, adjust the pH to 8.0 with 4mol / L NaOH solution, add a composite enzyme composition equivalent to 0.6‰ of the weight of the aloe vera leaf protein coarse powder, the The compound enzyme composition is a mixture of alkaline protease and neutral protease at a mass ratio of 1:2; placed in a constant temperature water bath at 50°C for 3.5 hours of e...
Embodiment 3
[0069] Plant polypeptides induce the migration and directed differentiation of vascular progenitor cells (VPC) into vascular endothelial cells
[0070] Vascular progenitor cells (VPC) were inoculated into migration holes with a pore size of 0.8 microns, and the base was added with 1 ng / mL of common polypeptide of the control group, 1 ng / mL of the sequence structure obtained from the natural plant Bletilla striata according to the present invention (1) and Sequence structure (2) (mass ratio 1:2) plant polypeptide composition, 10ng / mL vascular endothelial cell growth factor VEGF culture medium, observe and calculate the number of migrating cells passing through the 0.8 micron membrane after 16 hours. see results figure 1 and figure 2 . VEGF is recognized as a growth factor that promotes the differentiation, proliferation and migration of vascular endothelial cells, thereby promoting the formation of new blood vessels. figure 1 and figure 2 It shows that the sequence struct...
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