Polypeptide and application thereof

A peptide synthesis and sequence technology, applied in the direction of application, peptide, peptide source, etc., can solve the problems that are difficult to achieve effective control and complete cure, drug resistance reduces the growth performance of pigs, etc., achieves huge medicinal development potential, promotes The effect of uterine wound healing and improving the economic benefits of pig farms

Inactive Publication Date: 2016-10-26
胡国田 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the prevention and treatment of piglet diarrhea mainly starts from the two aspects of convergence and antibacterial, it is difficult to achieve effective control and complete cure, especially the use of antibiotics is easy to produce drug resistance, reduce the growth performance of pigs, etc.

Method used

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  • Polypeptide and application thereof
  • Polypeptide and application thereof
  • Polypeptide and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] (1) Take the natural plant Panax notoginseng and chop it up, homogenate it, add 0.06moL / L Tris-Hcl buffer solution with a pH of 8.0 equivalent to 8 times the volume of the plant raw material, sonicate for 4min, centrifuge to remove the slag, and take the supernatant solution, to obtain the crude protein extract of Panax notoginseng root;

[0043] (2) Precipitate the crude notoginseng root protein extract obtained in step (1) with ammonium sulfate, place it at 4°C overnight, then centrifuge, take the precipitate, and freeze-dry to obtain the notoginseng root protein coarse powder;

[0044] (3) Take notoginseng root protein powder, dissolve it in distilled water, adjust the pH to 8.0 with 5 mol / L NaOH solution, add a compound enzyme composition equivalent to 0.5‰ of vegetable protein meal weight, and the compound enzyme composition Mix alkaline protease and neutral protease at a mass ratio of 1:2; place in a constant temperature water bath at 50°C for 3 hours of enzymolys...

Embodiment 2

[0052] (1) Mince the aloe vera leaves, homogenate, add 0.06moL / L Tris-Hcl buffer solution with a pH of 8.5 equivalent to 6 times the volume of the plant material, sonicate for 3.5min, centrifuge to remove slag, and take the supernatant liquid, that is, the crude extract of aloe vera leaf protein;

[0053] (2) Precipitate the crude aloe leaf protein extract obtained in step (1) with ammonium sulfate, place it at 4°C overnight, then centrifuge, take the precipitate, and freeze-dry to obtain aloe leaf vegetable protein powder;

[0054] (3) Take the aloe vera leaf protein coarse powder obtained in step (2), dissolve it with distilled water, adjust the pH to 8.0 with 4mol / L NaOH solution, add a composite enzyme composition equivalent to 0.6‰ of the weight of the aloe vera leaf protein coarse powder, the The compound enzyme composition is a mixture of alkaline protease and neutral protease at a mass ratio of 1:2; placed in a constant temperature water bath at 50°C for 3.5 hours of e...

Embodiment 3

[0069] Plant polypeptides induce the migration and directed differentiation of vascular progenitor cells (VPC) into vascular endothelial cells

[0070] Vascular progenitor cells (VPC) were inoculated into migration holes with a pore size of 0.8 microns, and the base was added with 1 ng / mL of common polypeptide of the control group, 1 ng / mL of the sequence structure obtained from the natural plant Bletilla striata according to the present invention (1) and Sequence structure (2) (mass ratio 1:2) plant polypeptide composition, 10ng / mL vascular endothelial cell growth factor VEGF culture medium, observe and calculate the number of migrating cells passing through the 0.8 micron membrane after 16 hours. see results figure 1 and figure 2 . VEGF is recognized as a growth factor that promotes the differentiation, proliferation and migration of vascular endothelial cells, thereby promoting the formation of new blood vessels. figure 1 and figure 2 It shows that the sequence struct...

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Abstract

The invention discloses a polypeptide and application thereof. The polypeptide contains continuous amino acid residue fragments with sequences of Pro-Gly-Ala-Asp or Asp-Ala-Gly-Pro. The polypeptide can be synthesized according to sequence structures by the aid of a polypeptide synthesizer and also can be extracted from natural plants including radix notoginseng, flos sophorae, aloe vera and\or rhizoma bletillae. The polypeptide and the application have the advantages that effects of promoting migration of stem cells and vascular progenitor cells of mice and directionally differentiating the stem cells and the vascular progenitor cells towards vascular endothelial cells can be realized by the polypeptide, and accordingly damage repair of the vascular endothelia cells and formation of new blood vessels can be promoted; skin spray can be prepared from the polypeptide, wound healing can be promoted by the skin spray, and inflammation can be prevented and treated by the skin spray; oral liquid for piglets can be prepared from the polypeptide, regeneration and repair of intestinal villi of the piglets can be promoted by the oral liquid, and accordingly diarrhea diseases of the piglets can be fundamentally prevented and radically treated by the oral liquid; feed additives for animals can be prepared from the polypeptide, and effects of resisting diarrhea and promoting growth can be realized by the feed additives; perfusion agents for the uteruses of sow can be prepared from the polypeptide, quick healing of postpartum wound of the sow can be promoted by the perfusion agents, and inflammation of the sow can be effectively prevented and treated by the perfusion agents.

Description

technical field [0001] The present invention relates to a polypeptide and its application. Background technique [0002] In pig production, all pigs need to have their umbilicus cut off after birth, most of the piglets will have their tails docked, and half of the pigs need to be castrated. At the same time, they will face group fighting, tail biting, mechanical damage, hernia, prolapse and suture surgery, etc. If the wounds produced in pig production are not treated in time, it will cause infection of pathogens such as bacteria and viruses, which can cause local inflammation, redness and concurrent diseases, thereby affecting the growth performance of pigs, reducing carcass quality and even causing death. In particular, postpartum wounds of sows are not treated in time, which can easily lead to inflammation such as metritis and vaginitis in sows, resulting in repeated infertility, post-pregnancy abortion, pus discharge during estrus or before mating, etc., directly endanger...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C12P21/06C07K1/20C07K1/16A61K38/08A61K38/10A61K9/12A61K9/08A61P17/02A61P29/00A61P1/12A61P3/00A23K50/30A23K20/147
CPCA23K20/147A23K50/30A61K9/0014A61K9/0053A61K9/08A61K9/12A61K38/00C07K14/415C12P21/06
Inventor 胡国田曾灵芳胡瑕隆曙红袁朔烈杨强
Owner 胡国田
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