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A kind of fermentation medium of aureomycin

A fermentation medium, chlortetracycline technology, applied in the direction of fermentation, etc., can solve the problem of low chlortetracycline titer, achieve the effects of improving fermentation titer, solving high viscosity, and improving carbon source utilization

Active Publication Date: 2018-10-02
HEBEI SHENGXUE DACHENG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention provides a kind of aureomycin fermenting medium that can greatly improve the fermenting titer in order to solve the technical problem of the low titer of aureomycin fermented and cultivated in the fermented culture medium of aureomycin in the prior art

Method used

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  • A kind of fermentation medium of aureomycin
  • A kind of fermentation medium of aureomycin
  • A kind of fermentation medium of aureomycin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Culture medium configuration: Weigh each component according to the formula in Table 1, add purified water to dissolve, adjust the volume to 1000mL, and adjust the pH to 6.8; the numbers are experimental groups 1, 2, 3, 4, 5, 6, 7, 8, 9, the control group.

[0018] Table 1

[0019]

[0020] Subpackaging and sterilization: respectively divide 1000mL medium in the experimental group 1-9 and the control group into three shake flasks with a volume of 250mL, which are respectively recorded as shake flask 1, shake flask 2, and shake flask 3. The bottled volume is 50mL, and the remaining feed liquid is discarded. Add a 6g cotton plug, two layers of gauze, and a layer of kraft paper to wrap the head. Sterilize at 121°C for 30 minutes and cool to room temperature to obtain a fermentation medium. Inoculation: Insert the seed culture of the chlortetracycline-producing bacteria Streptomyces aureus into the fermentation medium prepared by the present embodiment according to the ...

Embodiment 2

[0025] Culture medium configuration: Weigh each component according to the formula in Table 3, add purified water to dissolve, adjust the volume to 1000mL, and adjust the pH to 6.8; the numbers are experimental group 1, 2, 3, and control group.

[0026] table 3

[0027]

[0028]

[0029] Subpackaging and sterilization: respectively subpackage 1000mL culture medium in experimental group 1-3 and control group into 5 shake flasks with a volume of 250mL, each bottle contains 50mL, discard the remaining feed liquid, add 6g cotton plug, Two layers of gauze, one layer of kraft paper wrapping, sterilized at 121°C for 30 minutes, cooled to room temperature, to obtain fermentation medium.

[0030] Inoculation: Insert the seed culture of the chlortetracycline-producing bacteria Streptomyces aureus into the fermentation medium prepared by the present embodiment according to the inoculation amount of 10%, and detect the titer after cultivating at a temperature of 29-31° C. for 120 ho...

Embodiment 3

[0034] Culture medium configuration: Weigh each component according to the formula in Table 5, add purified water to dissolve, adjust the volume to 1000mL, and adjust the pH to 6.8; the numbers are experimental group 1, 2, 3, and control group, respectively.

[0035] table 5

[0036]

[0037] Subpackage and sterilize: Distribute 1000mL of medium in the experimental group 1-3 and the control group respectively into 3 shake flasks with a volume of 250mL, each bottle contains 50mL, discard the remaining feed liquid, add 6g of cotton plug, Two layers of gauze, one layer of kraft paper wrapping, sterilized at 121°C for 30 minutes, cooled to room temperature, to obtain fermentation medium.

[0038] Inoculation: Insert the seed culture of the chlortetracycline-producing bacteria Streptomyces aureus into the fermentation medium prepared by the present embodiment according to the inoculation amount of 10%, and detect the titer after cultivating at a temperature of 29-31° C. for 120 ...

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Abstract

The invention provides a fermentation medium of aureomycin, belonging to the technical field of fermentation culture. The fermentation medium comprises the following components by weight: 25-35g / L of glucose, 50-70g / L of vegetable oil, 30-50g / L of soybean cake meal, 5-7g / L of calcium carbonate, 20-30g / L of corn steep liquor, 4-6g / L of ammonium sulfate, 3-5g / L of sodium chloride, 0.2-0.3g / L of monopotassium phosphate and 0.2-0.3g / L of magnesium sulfate. The fermentation medium has the beneficial effects that the traditional concept in the fermentation field is broken through; plenty of oil is used in the fermentation medium, so that not only is the carbon source availability of strains increased but also the problem of foam generation in the fermentation process is solved and the fermentation titer is also substantially increased, specifically, the shake flask titer after aureomycin is fermented for 120 hours is more than 29000U / mL and the highest shake flask titer can be 31502U / mL.

Description

technical field [0001] The invention belongs to the technical field of fermentation culture, relates to the configuration of a fermentation medium, in particular to a fermentation medium of aureomycin, and the fermentation medium of the invention can greatly increase the titer when used for producing aureomycin. Background technique [0002] Chlortetracycline is a tetracyclic antibiotic, which is obtained by two-stage fermentation of Streptomyces aureus and drying. Chlortetracycline has a broad-spectrum antibacterial effect, and its antibacterial spectrum is similar to that of tetracycline. The main active ingredient in aureomycin premix is ​​aureomycin Korean salt, which has antibacterial effect on most Gram-positive bacteria and Gram-negative bacteria. Chlortetracycline premix is ​​generally added to livestock feed to inhibit the growth of bacteria and improve feed conversion rate. Chlortetracycline hydrochloride has a therapeutic effect and is generally used in drinking...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P29/00
Inventor 赵丽红齐巧娟
Owner HEBEI SHENGXUE DACHENG PHARMA