A strain of porcine pseudorabies virus

Abstract

The invention discloses a porcine pseudorabies virus strain, which is characterized in that: porcine pseudorabies virus (Herpesviridae) strain, CCTCC NO: V201314. The present invention passes the PRV / HN2012 strain to the 7th generation, and carries out the virus titer TCID to the 7th generation virus 50 The results of the determination of TCID 50 for 10 9.0 . The PRV / HN2012 strain was inoculated into mice. After 24 hours of challenge, the mice in the PRV / HN2012 strain-infected group developed neurological symptoms. After 48 hours of challenge, all the mice died, indicating that the strain has strong toxicity. Based on this, through the research on the physical and chemical characteristics of PRV / HN2012 strain, the results show that the strain is sensitive to analytical pure chloroform and belongs to enveloped virus; it has strong resistance to acid and alkali, hydrochloric acid at pH 3.0, and It was inactivated by NaOH treatment for 1 h; it had strong resistance to heat and could be inactivated by treatment in a water bath at 56°C for 1 h; it was sensitive to trypsin and had no obvious effect on its infectivity after being treated with ultraviolet light for 30 min.

Description

technical field [0001] The invention relates to a porcine pseudorabies virus strain. Background technique [0002] Pseudorabies (PR) is an acute infectious disease caused by pseudorabies virus (PRV) in domestic animals and a variety of wild animals, with fever, itching (except pigs) and encephalomyelitis as the main symptoms . The disease was first seen in the United States in 1813. Because its clinical manifestations are similar to rabies, it is called pseudorabies. In 1902, the Hungarian scholar Aujeszky first reported the disease, so the disease was also named Aujeszky's disease. Pigs are the natural host and main source of infection of the disease. PRV can infect pigs of different ages, but the most serious infection is in pregnant sows and suckling piglets: leading to abortion, stillbirth and mummified fetuses in pregnant sows; obvious neurological symptoms in newborn piglets Symptoms, paralysis, exhaustion and death are mostly acute fatal processes, and the mortalit...

AI Technical Summary

Problems solved by technology

Since the spring of 2012, there have been suspected cases of PR in pig farms immunized with PRV gene-deficient vaccines in Henan, Jilin, Heilongjiang and other areas. % to 50%, causing huge economic losses to my country's pig industry

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