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Novel natural compound and preparation and application thereof

A compound and natural technology, applied in the field of medicine, can solve the problems such as anti-tumor activity of no cannabinoid saponin monomer components, and achieve the effect of strong anti-cancer activity and growth inhibition

Active Publication Date: 2016-01-06
YUNNAN PHYTOPHARML
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the isolation of cannabinoid saponin C from cannabis, and there is no report of cannabinoid saponin C in the online version of the chemical abstract database Scifinder data search and literature search. It is a new natural compound and has not been carried out so far. Reports on the in-depth research on the activity of monomeric components of cannabis saponins, especially reports on the anti-tumor activity of monomeric components of cannabis saponins

Method used

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  • Novel natural compound and preparation and application thereof
  • Novel natural compound and preparation and application thereof
  • Novel natural compound and preparation and application thereof

Examples

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Effect test

Embodiment 1

[0018] Example 1: Component Separation

[0019] Cannabis medicine 10Kg, crushed and extracted 3 times with 70% ethanol under heat reflux, 1250g of concentrated extract under reduced pressure, eluted with chloroform-methanol gradient through silica gel column chromatography, the gradient is chloroform:methanol volume ratio is 50:1 , 30:1, 20:1, 10:1, 5:1, 3:1, 1:1, tested by TLC, the same parts were merged together and divided into 6 parts, numbered Fr. 2. Fr.3, Fr.4, Fr.5, Fr.6; among them, Fr.3 was separated and purified by silica gel column chromatography and Sephadex column chromatography to obtain 500 mg of the compound, which was named as cannabis drug saponin C.

Embodiment 2

[0020] Example 2: Structural Identification of Cannabis Saponin C

[0021] Cannabis saponin C is a white powder, soluble in methanol. In order to determine its chemical structure, the following spectra were made for cannabis saponin C: (1) spectrum, optical rotation spectrum, ultraviolet spectrum and infrared spectrum, (2) nuclear magnetic resonance one-dimensional spectrum Done 1 H. 13 C and DEPT spectra, (3) HMBC, HSQC and 1 H- 1 HCOSY spectrum, (4) NOE experiment.

[0022] The entire structure identification process is described in detail below: According to the one-dimensional nuclear magnetic spectrum 1 H. 13 CNMR and DEPT spectrum, preliminarily judged to be a triterpene saponin, through literature research, Pu Xiangyu and others had isolated the triterpene saponin cannabinoid glycoside A (DolirosideA) from this plant, the NMR data of the two have a great deal similarities. According to the quasi-molecular ion peak 671 ([M+Na]+) given by ESI+-MS, HRESIMS ([M+Na]+,...

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PUM

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Abstract

The invention discloses a novel natural compound and preparation and application thereof. The compound is novel in structure and high in anti-tumor activity and is hemp medicine separated from dolichos falcate klein roots. The invention further discloses a preparation method of the novel natural compound. The compound is obtained through alcohol extraction of hemp medicine coarse powder, vacuum concentration, column chromatography on silica gel, chloroform-methyl alcohol gradient elution and separation and purification. The novel compound is white powder seen from the appearance and is triterpene saponin, the molecular formula is C36H56O10, and the novel compound is named hemp medicine saponin C.

Description

technical field [0001] The invention belongs to the technical field of medicine, and specifically relates to a new natural compound structure and its preparation method and application. Background technique [0002] Cannabis medicine is the root of Dolichos falcata Klein, a plant of the genus Fabaceae. It is commonly known as Mali Ma, Ma Sanduan, and Bean Leaf Baibu Huanyang. It is used medicinally in Yunnan, Guizhou and other places. It has the effects of dispelling wind, promoting blood circulation, stopping bleeding and relieving pain. . There are few studies on the chemical composition and pharmacological activity of cannabis medicine through literature review. Pu Xiangyu et al. have conducted research on the chemical composition of it and found that the main saponin component is cannabis medicine saponin A [Pu Xiangyu et al., Triterpenoids of Cannabis Medicine Saponins (1), Yunnan Plant Research 1984, 6(3)321-324]. In the 1970s and 1980s, Guiyang Medical College, Huan...

Claims

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Application Information

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IPC IPC(8): C07J63/00C07H15/256C07H1/08A61K31/704A61P35/00
Inventor 谢忠浪武正才吕小波黄春球腾利兵
Owner YUNNAN PHYTOPHARML