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Method for extracting ursolic acid and oleanolic acid from blueberry fruits

A technology of oleanolic acid and ursolic acid, applied in the direction of steroids, organic chemistry, etc., can solve the problems of few reports on the extraction and refining process of ursolic acid and oleanolic acid, and achieve low cost and simple extraction process , Environmentally friendly effect

Inactive Publication Date: 2016-01-06
SHANDONG INST OF POMOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, most of the researches on blueberries focus on anthocyanins, but there are few reports on the extraction and refining process of ursolic acid and oleanolic acid in blueberries.

Method used

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  • Method for extracting ursolic acid and oleanolic acid from blueberry fruits

Examples

Experimental program
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Embodiment 1

[0026] Select high-quality blueberry fruit as a raw material, rinse it with clean water, control the outer moisture after rinsing, and then dry the blueberry fruit with the outer moisture controlled at 55°C for 1.0h. The dried blueberry fruit is crushed into particles with a diameter of 80 mesh. Mix the crushed blueberry fruit with ethanol solution, the ethanol solution is used as the extractant, the mass percentage concentration is 90%, the solid-liquid ratio of the crushed blueberry fruit and the ethanol solution is adjusted to be 1:20, and the extraction is carried out under ultrasonic conditions for 45min. to 50W to obtain an extract. The extract is subjected to centrifugation for solid-liquid separation at a rotational speed of 4000 rpm, the residue is removed by filtration, the supernatant is collected, and the supernatant is adsorbed on a macroporous adsorption resin column. The polystyrene type macroporous resin is filled in the macroporous adsorption resin column, an...

Embodiment 2

[0028] Select high-quality blueberry fruit as a raw material, rinse it with clean water, control the outer moisture after rinsing, and then dry the blueberry fruit with the outer moisture controlled at 50°C for 1.0h. The dried blueberry fruit is crushed into particles with a diameter of 80 mesh. Mix the crushed blueberry fruit with ethanol solution, the ethanol solution is used as the extractant, the mass percentage concentration is 100%, the solid-liquid ratio of the crushed blueberry fruit and the ethanol solution is adjusted to be 1:15, and the extraction is carried out under ultrasonic conditions for 60min. to 150W to obtain an extract. The extract was subjected to centrifugation for solid-liquid separation at a centrifugation speed of 8000 rpm, the residue was removed by filtration, the supernatant was collected, and the supernatant was adsorbed on a macroporous adsorption resin column. The polystyrene type macroporous resin is filled in the macroporous adsorption resin ...

Embodiment 3

[0030] Select high-quality blueberry fruit as a raw material, rinse it with clean water, control the outer moisture after rinsing, and then dry the blueberry fruit with the outer moisture controlled at 60°C for 1.0h. The dried blueberry fruit is crushed into particles with a diameter of 80 mesh. Mix the crushed blueberry fruit with ethanol solution, the ethanol solution is used as the extractant, the mass percentage concentration is 80%, the solid-liquid ratio between the crushed blueberry fruit and the ethanol solution is adjusted to be 1:25, and the extraction is carried out under ultrasonic conditions for 30min. to 100W to obtain an extract. The extract was subjected to centrifugation for solid-liquid separation at a rotational speed of 3000 rpm, the residue was removed by filtration, the supernatant was collected, and the supernatant was adsorbed on a macroporous adsorption resin column. The polystyrene type macroporous resin is filled in the macroporous adsorption resin ...

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Abstract

The invention discloses a method for extracting ursolic acid and oleanolic acid from blueberry fruits. The method comprises the following steps that the blueberry fruits are mixed with an ethanol solution, extraction is carried out under the ultrasonic conditions, and an extracting solution is obtained; the extracting solution is subjected to centrifugal solid-liquid separation, supernate and solid residues are obtained, and the supernate is adsorbed to a macroporous adsorption resin column; elution is firstly carried out through water, then elution is carried out through an ethanol solution, and eluant is collected; the eluant is condensed and dried, and the ursolic acid and the oleanolic acid are obtained. The ursolic acid and the oleanolic acid are extracted from the blueberry fruits, and a new path is provided for blueberry resource development. Ultrasonic extraction, macroporous resin enrichment and other extraction steps are adopted, the purity of the ursolic acid and the oleanolic acid can be above 95%, and the advantages that the extracting process is simple, no large equipment is needed, adopted reagents are free of toxins, environment friendliness is achieved, cost is low, and energy consumption is greatly reduced are achieved.

Description

technical field [0001] The invention relates to a method for extracting ursolic acid and oleanolic acid, in particular to a method for extracting ursolic acid and oleanolic acid from blueberry fruit. Background technique [0002] Blueberry, English name: Blueberry, means blue berry, belongs to Rhododendronaceae, Vaccinium. Originated in North America. Blueberry fruit is rich in nutrients, which can prevent the aging of brain nerves, protect eyesight, strengthen the heart, fight cancer, soften blood vessels, and enhance human immunity. Blueberry fruit contains a variety of effective active ingredients such as anthocyanins, phenolic acids, ursolic acid and oleanolic acid. Ursolic acid, also known as ursolic acid, has a molecular formula of C30H18O3 and a molecular weight of 456.68. It is a pentacyclic triterpenoid compound that exists in many natural plants. It has a variety of biological activities, including anti-hepatitis, anti-tumor, and anti-AIDS Virus, antibacterial, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J63/00
Inventor 陈新徐丽刘庆忠魏海蓉宗晓娟朱东姿王甲威谭钺
Owner SHANDONG INST OF POMOLOGY
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