Petroleum hydrocarbon degradation bacterium and application thereof

A technology for degrading bacteria and petroleum hydrocarbons, applied in the direction of bacteria, water/sludge/sewage treatment, biochemical equipment and methods, etc., can solve the problems of secondary pollution of chemical reagents, damage to soil structure, difficult to implement, etc., and achieve good degradation Effects, broad application prospects, and simple cultivation methods

Inactive Publication Date: 2016-01-06
HARBIN ENG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The physical method of incineration destroys the soil structure and is expensive and difficult to implement; the chemical reage...

Method used

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  • Petroleum hydrocarbon degradation bacterium and application thereof
  • Petroleum hydrocarbon degradation bacterium and application thereof
  • Petroleum hydrocarbon degradation bacterium and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1: Degradation effect of bacterial strains on petroleum hydrocarbons under different initial test oil concentrations

[0034] 1. Take the colony from the slant surface of the strain preservation medium stored at 4°C and spread it on the activation medium plate. After culturing at 30°C for 24-36 hours, pick a single colony and inoculate it into the seed liquid medium, 30°C, 200r / min Incubate on a shaker for 12 hours. OD 600 Measure the concentration of the bacterial suspension under the wavelength and dilute the bacterial suspension to 0.8, and use it as a seed solution.

[0035] 2. Prepare degradation medium with initial oil concentration of 100, 200, 500, 1000, 1500, 2000, 3000 mg / L respectively.

[0036] 3. Inoculate the seed liquid of the strain into the degradation medium with different oil concentrations according to the inoculation amount of 5%, and make three parallel samples for each concentration, and the medium without inoculation is used as a blank ...

Embodiment 2

[0038] Example 2: Degradation effect of bacterial strains on petroleum hydrocarbons in shake flasks under different salinities

[0039] 1. Take the colony from the slant surface of the strain preservation medium stored at 4°C and spread it on the activation medium plate. After culturing at 30°C for 24-36 hours, pick a single colony and inoculate it into the seed liquid medium, 30°C, 200r / min Incubate on a shaker for 12 hours. OD 600 Measure the concentration of the bacterial suspension under the wavelength and dilute the bacterial suspension to 0.8, and use it as a seed solution.

[0040] 2. Preparation of degradation medium with salt concentrations of 0.5%, 1.0%, 1.5%, 2.0%, 2.5%, and 3.0%, respectively, and oil concentration of 200 mg / L.

[0041] 3. Inoculate the seed solution of the strain into the degradation medium with different salinities according to the inoculum amount of 5%, and make three parallel samples for each salt concentration, and the medium without inocula...

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PUM

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Abstract

The invention relates to a petroleum hydrocarbon degradation bacterium and application thereof. The petroleum hydrocarbon degradation bacterium is identified to belong to Acinetobacter sp, named as Acinetobacter.Bill, and preserved in China General Microbiological Culture Collection Center with the preservation number of CGMCC No.10283. BLAST retrieval comparison of the sequence of the bacterial strain in Genbank discovers that the sequence is highly homologous with the gene sequence of Acinetobacter sp bacterial strain. The bacterial strain of the petroleum hydrocarbon degradation bacterium is favorable in growth in a culture medium taking petroleum hydrocarbon as the only carbon source, has a favorable degradation effect on petroleum hydrocarbon, and meanwhile, has favorable adaptive capacity to salinity. The petroleum hydrocarbon degradation bacterium produced according to the technical scheme can efficiently degrade petroleum hydrocarbon in the environment to protect the ecological environment.

Description

technical field [0001] The invention relates to a bacterial strain for efficiently degrading petroleum hydrocarbons obtained by self-enrichment and separation from activated sludge collected from a biochemical treatment pool of oil production wastewater. The invention also relates to the application of a bacterial strain for degrading petroleum hydrocarbons. Background technique [0002] The main component of petroleum is a mixture of various alkanes, cycloalkanes, and aromatic hydrocarbons, which are soluble in various organic solvents and insoluble in water. Petroleum is mainly used as fuel oil and gasoline, and is also a raw material for many chemical industry products such as solvents, fertilizers, pesticides and plastics. A variety of polycyclic aromatic hydrocarbons in petroleum components have strong "three-induced" effects, and their volatile components can cause human organ diseases. With the rapid development of my country's economy and the increase in demand for...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/32
Inventor 任芝军官涤张忠祥刘迁张志柳
Owner HARBIN ENG UNIV
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